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Zinc solubilizing bacteria (Bacillus megaterium) with multifarious plant growth promoting activities alleviates growth in Capsicum annuum L.

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Abstract

The present study was designed to isolate an array of zinc solubilizing bacteria (ZSB) and to characterize them for plant growth promotion (PGP) attributes with respect to Capsicum annuum L. For this purpose, seventy bacteria were procured from cow dung and screened for zinc solubilization (ZnO and ZnCO3). Where, isolate CDK25 was found to be the most potent owing to its maximum zinc solubilization (ZnO) ability (5.0 cm). For quantitative assay, atomic absorption spectroscopy (AAS) was used, where CDK25 showed markedly higher solubilization of ZnO (20.33 ppm). It was investigated that CDK25 also endowed with multiple PGP attributes viz., Phosphate solubilization, Phytase production, Indole acetic acid (IAA) and Siderophore production. Quantitative study revealed isolate CDK25 to solubilize and produce maximum quantity of phosphate (281.59 μg/ml) and IAA (13.8 μg/ml) respectively. ZSB was applied in different treatments under pot culture assay, where T3 (seeds + CDK25) showed significant impact on plant growth parameters, besides showing maximum zinc content in fruit (0.25 mg/100 g). Hence, isolate CDK25 expresses highest potential throughout the experiments; as zinc solubilizer, PGP strain, and based on 16S rRNA gene sequencing identified as Bacillus megaterium. Therefore, meticulous use of this bacterium could aid in providing adequate amount of soluble zinc along with enhanced plants growth, nutrient uptake and yield in sustainable manner.

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Acknowledgements

The authors thank Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar, Uttrakhand, India, for providing necessary facilities to carry out this research.

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  1. Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar, Uttarakhand, 249404, India

    Kalpana Bhatt & Dinesh Kumar Maheshwari

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  1. Kalpana Bhatt
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KB assisted during the experiments and prepared the manuscript. DKM corrected and finalized the manuscript.

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Correspondence to Kalpana Bhatt.

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Bhatt, K., Maheshwari, D.K. Zinc solubilizing bacteria (Bacillus megaterium) with multifarious plant growth promoting activities alleviates growth in Capsicum annuum L.. 3 Biotech 10, 36 (2020). https://doi.org/10.1007/s13205-019-2033-9

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