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Analysis of differential gene expression in cold-tolerant vs. cold-sensitive varieties of snap bean (Phaseolus vulgaris L.) in response to low temperature stress

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Abstract

Background

Snap bean, Phaseolus vulgaris L., as a warm-season vegetable, low temperature stress seriously affect the yield and quality. At present, little is known about the genes and molecular regulation mechanism in cold response in snap bean exposed to low temperature.

Objectives

Our objectives were to identify the low temperature response genes in snap bean and to examine differences in the gene response between cold-tolerant and cold-sensitive genotypes.

Methods

We used two highly inbred snap bean lines in this study, the cold-tolerant line ‘120’, and the cold-sensitive line ‘093’. The plants were grown to the three leaf and one heart stage and exposed to 4 °C low temperature. We used RNA sequencing (RNA-seq) to analyze the differences of gene expression.

Results

988 and 874 cold-responsive genes were identified in ‘T120 vs CK120’ and ‘T093 vs CK093’ (‘T’ stands for low temperature treatment, and ‘CK’ stands for control at room temperature), respectively. Of these, 555 and 442 genes were unique to cold-stressed lines ‘120’ and ‘093’, respectively compared to the control. Our analysis of these differentially expressed genes indicates that Ca2+, ROS, and hormones act as signaling molecules that play important roles in low temperature response in P. vulgaris. Altering the expression of genes in these signaling pathways activates expression of downstream response genes which can interact with other signaling regulatory networks. This may maintained the balance of ROS and hormones, making line ‘120’ more cold-tolerant than line ‘093’.

Conclusion

Our results provide a preliminarily understanding of the molecular basis of low temperature response in snap bean, and also establish a foundation for the future genetic improvement of cold sensitivity in snap bean by incorporating genes for cold tolerance.

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Funding

Financial support was provided by the China Postdoctoral Science Foundation (Grant number 2018M631973); the Basic scientific research operating expenses of provincial College in Heilongjiang province (Grant numbers KJCXYB201707 and KJCXYB201705); the National Natural Science Funds of China (Grant number 31771869); the Applied technology research and development plan of Heilongjiang province (Grant number GY2019YF0059 ).

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Author notes

  1. Chang Liu and Xiaoxu Yang contributed equally to this work.

Authors and Affiliations

  1. Horticulture Department, Academy of Crop Science, Heilongjiang University, 74 Xuefu Road, Harbin, 150000, Heilongjiang, China

    Chang Liu, Xiaoxu Yang, Zhishan Yan, Youjun Fan, Guojun Feng & Dajun Liu

  2. Work Station of Science and Technique for Post-doctoral in Sugar Beet Institute Affiliated to Heilongjiang University, 74 Xuefu Road, Harbin, 150000, Heilongjiang, China

    Chang Liu & Xiaoxu Yang

  3. Post-doctoral Research Station Affiliated To Northeast Agricultural University, 59 Mucai Road, Harbin, 150000, Heilongjiang, China

    Chang Liu & Xiaoxu Yang

Authors
  1. Chang Liu
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  2. Xiaoxu Yang
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  3. Zhishan Yan
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  4. Youjun Fan
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Correspondence to Guojun Feng or Dajun Liu.

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Liu, C., Yang, X., Yan, Z. et al. Analysis of differential gene expression in cold-tolerant vs. cold-sensitive varieties of snap bean (Phaseolus vulgaris L.) in response to low temperature stress. Genes Genom 41, 1445–1455 (2019). https://doi.org/10.1007/s13258-019-00870-2

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