Abstract
Drought stress is one of the major abiotic stresses that impair the growth and production of canola plants in arid and semi-arid zones, which necessitates the development of drought-resilient cultivars. The study aimed at determining the morphological, biochemical, and physiological responses of six different canola cultivars under three drought stresses imposed by polyethylene glycol. The main and interaction effects of cultivar and drought stress were determined using analysis of variance. The results showed a considerable decline in root length due to drought stress regardless of the cultivar. A significant interaction between cultivar and stress was detected on dry weight, height, and leaf area traits. Accordingly, growth parameters were observed to be linked with drought tolerance, and Hyola308 and Sarigol were identified as the most drought tolerant and sensitive cultivars, respectively. The values of chlorophyll contents in Hyola308 cultivar were remarkably higher than those in Sarigol under moderate-drought stress, while Sarigol maintained higher chlorophyll contents under severe stress. Increasing drought stress enhanced proline content and antioxidant enzyme activities (catalase and peroxidase) regardless of the cultivar. But a significant decrease in total soluble sugar was observed as drought intensity increased. This reduction in total soluble sugar content was about 38% lower in Hyola308 than in Sarigol. Therefore, some canola cultivars might mitigate drought stress effects by enhancing their proline and antioxidant system during drought stress. Furthermore, maintaining higher soluble sugar levels and greenness stability in response to increased drought stress might be a part of Hyola308’s cultivar stress management strategy.
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The data used to support the findings of this study are available from the corresponding author upon a reasonable request.
References
Ahmadi H, Abbasi A, Taleei A, Mohammadi V, Pueyo JJ (2022) Antioxidant response and calcium-dependent protein kinases involvement in canola (Brassica napus L.) tolerance to drought. Agronomy 12:125. https://doi.org/10.3390/agronomy12010125
Aliyari Rad S, Dehghanian Z, Asgari Lajayer B, Nobaharan K, Astatkie T (2021) Mitochondrial respiration and energy production under some abiotic stresses. J Plant Growth Regul 41:3285–3299. https://doi.org/10.1007/s00344-021-10512-1
Arnon AN (1967) Method of extraction of chlorophyll in the plants. Agron J 23:112–121
Ayyaz A, Miao Y, Hannan F, Islam F, Zhang K, Xu J, Farooq MA, Zhou W (2021) Drought tolerance in Brassica napus is accompanied with enhanced antioxidative protection, photosynthetic and hormonal regulation at seedling stage. Physiol Plant 172:1133–1148. https://doi.org/10.1111/ppl.13375
Banaei-Asl F, Bandehagh A, Uliaei ED, Farajzadeh D, Salzata K, Mustafa G, Komatsu S (2015) Proteomic analysis of canola root inoculated with bacteria under salt stress. J Proteom 124:88–111. https://doi.org/10.1016/j.jprot.2015.04.009
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207. https://doi.org/10.1007/BF00018060
Blum A (2017) Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant Cell Environ 40:4–10. https://doi.org/10.1111/pce.12800
Chance B, Maehly AC (1955) Assay of catalase and peroxidase. Method Enzymol 2:764–775. https://doi.org/10.1016/S0076-6879(55)02300-8
Channaoui S, El Kahkahi R, Charafi J, Mazouz H, El Fechtali M, Nabloussi A (2017) Germination and seedling growth of a set of rapeseed (Brassica napus) varieties under drought stress conditions. Int J Envir Agric Biotechnol 2:487–494. https://doi.org/10.22161/ijeab/2.1.61
Channaoui S, Idrissi ISE, Mazouz H, Nabloussi A (2019) Reaction of some rapeseed (Brassica napus L.) genotypes to different drought stress levels during germination and seedling growth stages. OCL 26:23. https://doi.org/10.1051/ocl/2019020
Chérifi K, Haddioui A, El-Hansali M, Boufous EH (2016) Growth and proline content in NaCl stressed plants of annual medic species. Int J Adv Res Biol Sci 3:82–90. https://doi.org/10.48550/arXiv.1609.07140
Clausen JJ, Kozlowski TT (1965) Use of the relative turgidity technique for measurement of water stresses in gymnosperm leaves. Can J Bot 43:305–316. https://doi.org/10.1139/b65-032
Couée I, Sulmon C, Gouesbet G, El Amrani A (2006) Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot 57:449–459. https://doi.org/10.1093/jxb/erj027
Dien DC, Mochizuki T, Yamakawa T (2019) Effect of various drought stresses and subsequent recovery on proline, total soluble sugar and starch metabolisms in Rice (Oryza sativa L.) varieties. Plant Prod Sci 22:530–545. https://doi.org/10.1080/1343943X.2019.1647787
Din J, Khan SU, Ali I, Gurmani AR (2011) Physiological and agronomic response of canola varieties to drought stress. J Animal Plant Sci 21:78–82
Elferjani R, Soolanayakanahally R (2018) Canola responses to drought, heat, and combined stress: shared and specific effects on carbon assimilation, seed yield, and oil composition. Front Plant Sci 9:1224. https://doi.org/10.3389/fpls.2018.01224
Fang Y, Xiong L (2015) General mechanisms of drought response and their application in drought resistance improvement in plants. Cell Mol Life Sci 72:673–689. https://doi.org/10.1007/s00018-014-1767-0
Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29:185–212. https://doi.org/10.1051/agro:2008021
García-Valenzuela X, García-Moya E, Rascón-Cruz Q, Herrera-Estrella L, Aguado-Santacruz GA (2005) Chlorophyll accumulation is enhanced by osmotic stress in graminaceous chlorophyllic cells. Plant Physiol 162:650–661. https://doi.org/10.1016/j.jplph.2004.09.015
Hasanuzzaman M, Bhuyan MHMB, Zulfiqar F, Raza A, Mohsin SM, Mahmud JA, Fujita M, Fotopoulos V (2020) Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator. Antioxidants 9:681. https://doi.org/10.3390/antiox9080681
Hellal F, Amer A, Azab KEL, Zewainy R (2018) Impact of irrigation water salinity on germination and seedling growth of Egyptian barley cultivars. J Agric Sci Technol 8:290–302. https://doi.org/10.17265/2161-6264/2018.05.003
Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012) Role of proline under changing environments: a review. Plant Signal Behav 7:1456–1466. https://doi.org/10.4161/psb.21949
Heidari F, Bandehagh A, Farajzadeh D, Kazemi Oskuei B, Motie Noparvar P (2015) Response of spring canola (Brassica napus L.) cultivars inoculated with P. fluorescens FY 32 to drought stress. Crop Res 50:55–62
Heshmat K, Asgari Lajayer B, Shakiba MR, Astatkie T (2021) Assessment of physiological traits of common bean cultivars in response to water stress and molybdenum levels. J Plant Nutr 44(3):366–372. https://doi.org/10.1080/01904167.2020.1822395
Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil, 347th edn. California Agricultural Experiment Station, Davis
Jamshidi Zinab A, Hasanloo T, Naji AM, Delangiz N, Farhangi Abriz S, Asgari Lajayer B, Hemati A, Shobbar ZS, Farooq M (2022) Physiological and biochemical evaluation of commercial oilseed rape (Brassica napus L.) cultivars under drought stress. Gesunde Pflanzen. https://doi.org/10.1007/s10343-022-00755-7
Khadem Moghadam N, Motesharezadeh B, Maali-Amiri R, Asgari Lajayer B, Astatkie T (2020) Effects of potassium and zinc on physiology and chlorophyll fluorescence of two cultivars of canola grown under salinity stress. Arab J Geosci 13:771. https://doi.org/10.1007/s12517-020-05776-y
Khaleghi A, Naderi R, Brunetti C (2019) Morphological, physiochemical and antioxidant responses of Maclura pomifera to drought stress. Sci Rep 9:19250. https://doi.org/10.1038/s41598-019-55889-y
Khan F, Upreti P, Singh R, Shukla PK, Shirke PA (2017) Physiological performance of two contrasting rice varieties under water stress. Physiol Mol Biol Plants 23:85–97. https://doi.org/10.1007/s12298-016-0399-2
Khan R, Ma X, Zhang J, Wu X, Iqbal A, Wu Y, Zhou L, Wang S (2021) Circular drought-hardening confers drought tolerance via modulation of the antioxidant defense system, osmoregulation, and gene expression in tobacco. Physiol Plant 172:1073–1088. https://doi.org/10.1111/ppl.13402
Khodabin G, Tahmasebi Sarvestani Z, Rad AHS, Modarres Sanavy SAM (2020) Effect of drought stress on certain morphological and physiological characteristics of a resistant and a sensitive canola cultivar. Chem Biodivers 17:e1900399. https://doi.org/10.1002/cbdv.201900399
Kochert G (1978) Carbohydrate determination by the phenol sulfuric acid method. In: Helebust JA, Craig JS (eds) Handbook physiological methods. Cambridge University Press, London, pp 95–97
Koroi SAA (1989) Gelekektrophers tische and spectral photometrischoe under uchungen zomeinfiuss der temperature auf straktur and aktritat der amylase and peroxidase isoenzym. Physiology 20:15–23
Kuczynska P, Jemiola-Rzeminska M, Strzalka K (2015) Photosynthetic pigments in diatoms. Mar Drugs 13:5847–5881. https://doi.org/10.3390/md13095847
Laxa M, Liebthal M, Telman W, Chibani K, Dietz KJ (2019) The role of the plant antioxidant system in drought tolerance. Antioxidants 8:94. https://doi.org/10.3390/antiox8040094
Li W, Shi Y, Zhu D, Wang W, Liu H, Li J, Shi N, Ma L, Fu S (2021) Fine root biomass and morphology in a temperate forest are influenced more by the nitrogen treatment approach than the rate. Ecol Indic 130:108031. https://doi.org/10.1016/j.ecolind.2021.108031
Li J, Charles LS, Yang Z, Du G, Fu S (2022) Differential mechanisms drive species loss under artificial shade and fertilization in the alpine meadow of the Tibetan plateau. Front Plant Sci 13:832473. https://doi.org/10.3389/fpls.2022.832473
Luo YZ, Li G, Yan G, Liu H, Turner NC (2020) Morphological features and biomass partitioning of lucerne plants (Medicago sativa L.) subjected to water stress. Agronomy 10:322. https://doi.org/10.3390/agronomy10030322
Meena M, Divyanshu K, Kumar S, Swapnil P, Zehra A, Shukla V, Yadav M, Upadhyay RS (2019) Regulation of L-proline biosynthesis, signal transduction, transport, accumulation and its vital role in plants during variable environmental conditions. Heliyon 5:e02952. https://doi.org/10.1016/j.heliyon.2019.e02952
Mohammadi Alagoz S, Zahra N, Hajiaghaei Kamrani M, Asgari Lajayer B, Nobaharan K, Astatkie T, Siddique KH, Farooq M (2022) Role of root hydraulics in plant drought tolerance. J Plant Growth Regul. https://doi.org/10.1007/s00344-022-10807-x
Montgomery DC (2020) Design and analysis of experiments, 10th edn. Wiley, New York
Nahar S, Vemireddy LR, Sahoo L, Tanti B (2018) Antioxidant protection mechanisms reveal significant response in drought- induced oxidative stress in some traditional rice of Assam, India. Rice Sci 25:185–196. https://doi.org/10.1016/j.rsci.2018.06.002
Nicoli MC, Elizalde BE, Piotti A, Lerici CR (1991) Effect of sugars and maillard reaction products on polyphenol oxidase activity in food. J Food Biochem 15:169–184. https://doi.org/10.1111/j.1745-4514.1991.tb00153.x
Poonia V, Jha S, Goyal MK (2021) Copula based analysis of meteorological, hydrological and agricultural drought characteristics across indian river basins. Int J Clim 31:4637–4652. https://doi.org/10.1002/joc.7091
Qamer Z, Chaudhary MT, Du X (2021) Review of oxidative stress and antioxidative defense mechanisms in Gossypium hirsutum L. in response to extreme abiotic conditions. J Cotton Res 4:9. https://doi.org/10.1186/s42397-021-00086-4
Rana MS, Hasan MA, Bahadur MM, Islam MR (2017) Effect of polyethylene glycol induced water stress on germination and seedling growth of wheat (Triticum aestivum). Agriculturists 15:81–91. https://doi.org/10.3329/agric.v15i1.33431
Raza A, Razzaq A, Mehmood SS, Zou X, Zhang X, Lv Y, Xu J (2019) Impact of climate change on crops adaptation and strategies to tackle its outcome: a Review. Plants 8:34. https://doi.org/10.3390/plants8020034
Rolando JL, Ramirez DA, Yactayo W, Monneveux P, Quiroz R (2015) Leaf greenness as a drought tolerance related trait in potato (Solanum tuberosum L.). Environ Exp Bot 110:27–35. https://doi.org/10.1016/j.envexpbot.2014.09.006
Rustioni L, Bianchi D (2021) Drought increases chlorophyll content in stems of Vitis interspecific hybrids. Theor Exp Plant Physiol 33:69–78. https://doi.org/10.1007/s40626-021-00195-0
Sanchez-Rodriguez E, Rubio-Wilhelmi M, Cervilla LM, Blasco B (2010) Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Plant Sci 178:11. https://doi.org/10.1016/j.plantsci.2009.10.001
Santos R, Carvalho M, Rosa E, Carnide V, Castro I (2020) Root and agro-morphological traits performance in cowpea under drought stress. Agronomy 10:1604. https://doi.org/10.3390/agronomy10101604
Sarker U, Oba S (2018) Catalase, superoxide dismutase and ascorbate-glutathione cycle enzymes confer drought tolerance of Amaranthus tricolor. Sci Rep 8:16496. https://doi.org/10.1038/s41598-018-34944-0
SAS Institute Inc (2014) SAS/STAT 94 user’s guide. SAS Institute Inc, Cary
Sayyad-Amin P, Jahansooz MR, Borzouei A, Ajili F (2016) Changes in photosynthetic pigments and chlorophyll-a fluorescence attributes of sweet-forage and grain sorghum cultivars under salt stress. J Biol Phys 42:601–620. https://doi.org/10.1007/s10867-016-9428-1
Sofo A, Scopa A, Nuzzaci M, Vitti A (2015) Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. Int J Mol Sci 16:13561–13578. https://doi.org/10.3390/ijms160613561
Sun J, Jia Q, Li Y, Zhang T, Chen J, Ren Y, Dong K, Xu S, Shi NN, Fu S (2022) Effects of arbuscular mycorrhizal fungi and biochar on growth, nutrient absorption, and physiological properties of maize (Zea mays L.). J Fungi 8(12):1275. https://doi.org/10.3390/jof8121275
Toscano S, Scuderi D, Giuffrida F, Romano D (2014) Responses of mediterranean ornamental shrubs to drought stress and recovery. Sci Hortic 178:145–153. https://doi.org/10.1016/j.scienta.2014.08.014
Toscano S, Ferrante A, Romano D (2019) Response of mediterranean ornamental plants to drought stress. Horticulturae 5:6. https://doi.org/10.3390/horticulturae5010006
Wang X, Samo N, Li L, Wang M, Qadir M, Jiang K, Qin J, Rasul F, Yang G, Hu Y (2019) Root distribution and its impacts on the drought tolerance capacity of hybrid rice in the sichuan basin area of China. Agronomy 9:79. https://doi.org/10.3390/agronomy9020079
Weithmann G, Schuldt B, Link RM, Heil D, Hoeber S, John H, Muller-Haubold H, Schuller L-M, Schumann K, Leuschner C (2021) Leaf trait modification in European beech trees in response to climatic and edaphic drought. Plant Biol 24:1272–1286. https://doi.org/10.1111/plb.13366
Xie J, Yao S, Ming J, Deng L, Zeng K (2019) Variations in chlorophyll and carotenoid contents and expression of genes involved in pigment metabolism response to oleocellosis in citrus fruits. Food Chem 272:49–57. https://doi.org/10.1016/j.foodchem.2018.08.020
Xu W, Cui K, Xu A, Nie L, Huang J, Peng S (2015) Drought stress condition increases root to shoot ratio via alteration of carbohydrate partitioning and enzymatic activity in rice seedlings. Acta Physiol Plant 37:1–11. https://doi.org/10.1007/s11738-014-1760-0
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Kazemi Oskuei, B., Bandehagh, A., Farajzadeh, D. et al. Morphological, biochemical, and physiological responses of canola cultivars to drought stress. Int. J. Environ. Sci. Technol. 20, 13551–13560 (2023). https://doi.org/10.1007/s13762-023-04928-3
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DOI: https://doi.org/10.1007/s13762-023-04928-3