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In Vitro Plant Regeneration Potential of Genetically Stable Globba marantina L., Zingiberaceous Species and its Conservation

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

A protocol was developed for propagation and in vitro conservation of a rare traditional medicinal plant, Globba marantina L. The present slow growth protocol was achieved for long term conservation of plants using both solid and liquid Murashige and Skoog media with different carbon sources and growth hormones. Half-Murashige and Skoog supplemented with Kinetin (3 mg/l) and Naphthalene acetic acid (0.5 mg/l) was found to be optimum with sucrose (10 g/l) and mannitol (10 g/l). Subculture duration could be significantly enhanced and the regenerated plants were successfully conserved for a maximum period of 220 days. Plants regenerated from conserved cultures were successfully established in soil. On the basis of 12 inter simple sequence repeat primers and morphological characteristic analysis from field up to two generations, no significant variation was observed between the control and in vitro regenerated plants. The present protocol for the first time reports mass propagation and genetic stability of G. marantina L. which could be used for commercial purposes.

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References

  1. Kress WJ, Prince LM, Williams KJ (2002) The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. Am J Bot 89(10):1682–1696

    Article  CAS  PubMed  Google Scholar 

  2. Schumann K (1904) Zingiberaceae, In: A. Engler (ed), Das Pflanzenreich. Wilhelm Engelmann, Leipzig. 4(46):1–458

  3. Larsen KA (1996) Preliminary checklist of the Zingiberaceae of Thailand. Thai For Bull Bot 24:35–49

    Google Scholar 

  4. Kress WJ, Defilipps RA, Farr E, Kyi YY (2003) A checklist of the trees, shrubs, herbs, and climbers of Myanmar. Contrib U S Natl Herb 45:1–590

    Google Scholar 

  5. Swarnkar S, Katewa SS (2008) Ethnobotanical observation on tuberous plants from Tribal area of Rajasthan (India). Ethnobot leafl 12:647–666

    Google Scholar 

  6. Choudhary K, Singh M, Pillai U (2008) Ethnobotanical survey of Rajasthan—an update. Am Eurasian J Bot 1:38–45

    Google Scholar 

  7. Dagar HS (1989) Plant folk medicines among Nicobarese tribals of Car Nicobar Island, India. Econ Bot 43:215–224

    Article  Google Scholar 

  8. Engelmann F (1997) In vitro conservation methods. In: Callow JA, Ford BV, Newbury HJ (eds) Biotechnology and plant genetic resources: conservation and use, vol 19., Biotechnology in agriculture seriesCAB International, Oxford shire, pp 119–141

    Google Scholar 

  9. Withers LA, Williams JT (1986) In vitro conservation; international board of plant genetic resources—research highlights, 1984–8. IBPGR, Rome, p 21

    Google Scholar 

  10. Withers LA (1991) Biotechnology and plant genetic resources conservation. In: Paroda RS, Arora RK (eds) Plant genetic resources conservation and management concepts and approaches. IBPGR, New Delhi, p 273

    Google Scholar 

  11. Chandel KPS, Pandey R (1991) Plant genetic resources conservation—recent approaches. In: Paroda RS, Arora AK (eds) Plant genetic resources conservation and management concepts and approaches. IBPGR, Regional Office for South and Southeast Asia (ROSSEA), New Delhi, pp 248–272

    Google Scholar 

  12. Selvakkumar C, Balakrishnan A, Lakshmi BS (2007) Rapid in vitro micropropagation of Alpinia officinarum Hance, an important medicinal plant through rhizome bud explants. Asian J Plant Sci 6:1251–1255

    Article  CAS  Google Scholar 

  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  14. Doyle JJ, Doyle JL (1990) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  15. Zeitkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored PCR amplification. Genomics 20(2):176–183

    Article  Google Scholar 

  16. Rohlf FJ (1997) NTSYS-pc numerical taxonomy and multivariate analysis system Version2.02e. Exeter Software, Setauket

    Google Scholar 

  17. Anish NP, Dan M, Bejoy M (2008) Conservation using in vitro progenies of the threatened ginger—Boesenbergia pulcherrima (Wall.) Kuntze. Int J Bot 4(1):93–98

    Article  CAS  Google Scholar 

  18. Lincy AK, Remashree AB, Sasikumar B (2009) Indirect and direct somatic embryogenesis from aerial stem explants of ginger (Zingiber officinale Rosc.). Acta Bot Croat 68(1):93–103

    CAS  Google Scholar 

  19. Mohanty S, Panda MK, Sahoo S, Nayak S (2011) Micropropagation of Zingiber rubens and assessment of genetic stability through RAPD and ISSR analysis. Biol Plant 55(1):16–20

    Article  Google Scholar 

  20. Dekkers AJ, Rao AN, Ghosh CJ (1991) In vitro storage of multiple shoot culture of gingers at ambient temperatures of 24–29 °C. Sci Hortic (Amsterdam) 47:157–167

    Article  Google Scholar 

  21. Islam MA, Meister A, Schubert V, Kloppstech K, Esch E (2007) Genetic diversity and cytogenetic analyses in C. zedoaria (Christm.) Roscoe from Bangladesh. Genet Resour Crop Evol 54:149–156

    Article  Google Scholar 

  22. Sarwar M, Siddiqui SU (2004) In vitro conservation of sugarcane (Saccharum officinarum L.) germplasm. Pak J Bot 36:549–556

    Google Scholar 

  23. Karuppuswamy S, Rajasekaran KM (2009) High throughout antibacterial screening of plant extracts by resazurin redox with special reference to medicinal plants of Western ghats. Glob J Pharm 3(2):63–68

    Google Scholar 

  24. Dodds JH, Roberts LW (1995) Experiments in plant tissue culture. Cambridge University Press, New York 42

    Google Scholar 

  25. Geetha SP (2002) In vitro technology for genetic conservation of some genera of Zingiberaceae. PhD Thesis, Calicut University, Calicut

  26. Debergh PC, Read PE (1991) Micropropagation. In: Debergh PC, Zimmerman RH (eds) Micropropagation-technology and application. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 1–13

  27. Lavieville L, Herouart D, Sangwan RS, Sangwan BS (1991) Effect of storage at low temperature and under low oxygen on growth and alkaloid accumulation in callus cultures of Datura innoxa. Bull De la Soc Bot De France 137:6

    Google Scholar 

  28. Zamora AB, Gruezo SS, Rao AN, Rao VR (1999) Micropropagation and slow growth conditions for in vitro conservation of bamboo. In: Rao AN, Rao VR (eds) Bamboo and Rattan genetic resources and use. Proceedings of 3rd INBRI-IPGRI biodiversity, genetic resources and conservation working group meeting. IPGRI-APO, Serdang, Malaysia, pp 156–168

  29. Hassan NS (2004) Storage of in vitro banana shoot cultures at low temperature or under mineral oil layer. Int J Agric Biol 6:303–306

    Google Scholar 

  30. Sharma N, Satsangi R, Pandey R, Singh R, Kaushik N, Tyagi RK (2012) In vitro conservation of Bacopa monnieri (L.) using mineral oil. Plant Cell Tissue Organ Cult 111(3):291–301

    Article  CAS  Google Scholar 

  31. Geetha SP, Manjula C, Sajina A (1995) In vitro conservation of genetic resources of spices. In: Proceedings of the 7th Kerala science congress, vol 27. Palakkad, pp 12–16

  32. Balachandran SM, Bhat SR, Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma longa) and ginger (Zingiber officinale Rosc.). Plant Cell Rep 819:521–524

    Article  Google Scholar 

  33. Bhatia R, Singh KP, SharmaTR Jhang T (2011) Evaluation of the genetic fidelity of in vitro—propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers. Plant Cell Tissue Organ Cult 104:131–135

    Article  Google Scholar 

  34. Williams KJ, Kress WJ, Manos PS (2004) The phylogeny, evolution and classification of the genus Globba and tribe Globbeae (Zingiberaceae): appendages do matter. Am J Bot 91(1):100–114

    Article  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to Prof (Dr.) S.C. Si, Dean, Centre of Biotechnology and Prof (Dr.) M.R. Nayak, President, Siksha O Anusandhan University for providing facilities and encouraging throughout.

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  1. Centre of Biotechnology, Siksha O Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar, Odisha, 751003, India

    Reena Parida, Sujata Mohanty & Sanghamitra Nayak

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  1. Reena Parida
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  2. Sujata Mohanty
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  3. Sanghamitra Nayak
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Correspondence to Reena Parida.

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Parida, R., Mohanty, S. & Nayak, S. In Vitro Plant Regeneration Potential of Genetically Stable Globba marantina L., Zingiberaceous Species and its Conservation. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 321–327 (2018). https://doi.org/10.1007/s40011-016-0759-2

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  • DOI: https://doi.org/10.1007/s40011-016-0759-2

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