Abstract
This study aimed to use thyme volatile oil (TVO) to control potato bacterial wilt disease caused by Ralstonia solanacearum and to explore the biochemical responses in relation to defense enzymes that induce resistance against pathogens in plants. In vitro experiments showed that all tested concentrations of TVO (1 µl, 2, 4, 6, 8, and 10 µl ml−1) significantly (P ≤ 0.05) inhibited the growth of the pathogen, with the most significant effect recorded at a concentration of 10 µl ml−1. Under greenhouse conditions, the tested concentration of TVO (10 µl ml−1) significantly (P ≤ 0.05) reduced the incidence of potato bacterial wilt and the population of R. solanacearum pathogens in potato plants. The current study also revealed that TVO (10 µl ml−1) increased the activity of antioxidant enzymes, e.g.,. peroxidase (POX) and phenylalanine ammonia-lyase (PAL), and reduced the activity of catalase (CAT) in treated potato plants. In conclusion, our results showed that TVO (10 µl ml−1) afforded a strong reduction of R. solanacearum radial growth in vitro and a high reduction in the development of potato wilt in vivo. Moreover, we demonstrated that POX and PAL activities can be used as biochemical markers for biocontrol efficacy in the control of potato bacterial wilt.
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Authors are thankful to Taif University Researchers Supporting Project number (TURSP-2020/65), Taif University, Taif, Saudi Arabia for providing the financial support and research facilities.
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Kamal Abo-Elyousr, and Esmat F. Ali suggested the idea of the work and contributed to data curation and their validation as well as writing original draft. Nashwa Sallam, Mohame Breakia and Mohamed Seleim contributed to the formal analysis of the data all authors contributed to the reviewing and editing the manuscript. All authors reviewed and approved the final version of the manuscript.
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Sallam, N.M.A., Ali, E.F., Abo-Elyousr, K.A.M. et al. Thyme oil treatment controls bacterial wilt disease symptoms by inducing antioxidant enzyme activity in Solanum tuberosum. J Plant Pathol 103, 563–572 (2021). https://doi.org/10.1007/s42161-021-00808-2
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DOI: https://doi.org/10.1007/s42161-021-00808-2