Skip to main content

2019 | OriginalPaper | Buchkapitel

Ionic Liquids as Stabilization and Refolding Additives and Solvents for Proteins

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

This chapter focuses on recent advances in the use of ionic liquids as additives and solvents in protein applications. The solvent properties of ionic liquids can be tuned by the appropriate selection of cation and anion. The effects of different kinds of ionic liquids on protein stability and refolding behavior have been investigated and reported. The ionic liquid properties affect the intermolecular interactions of proteins, inducing different formations and folding behavior. These effects also vary with the concentration of ionic liquids. Although many of the associated mechanisms are not completely clear, some of this behavior may be attributed to the kosmotropicity of the ions and their Hofmeister effects.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Literatur
1.
Zurück zum Zitat Welton T (1999) Room-temperature ionic liquids. Solvents for synthesis and catalysis. Chem Rev 99:2071–2083CrossRef Welton T (1999) Room-temperature ionic liquids. Solvents for synthesis and catalysis. Chem Rev 99:2071–2083CrossRef
2.
Zurück zum Zitat Seddon KR (1997) Ionic liquids for clean technology. J Chem Technol Biotechnol 68:351–356CrossRef Seddon KR (1997) Ionic liquids for clean technology. J Chem Technol Biotechnol 68:351–356CrossRef
3.
Zurück zum Zitat Armand M, Endres F, MacFarlane DR, Ohno H, Scrosati B (2009) Ionic-liquid materials for the electrochemical challenges of the future. Nat Mater 8:621–629CrossRef Armand M, Endres F, MacFarlane DR, Ohno H, Scrosati B (2009) Ionic-liquid materials for the electrochemical challenges of the future. Nat Mater 8:621–629CrossRef
4.
Zurück zum Zitat Itoh T, Han SH, Matsushita Y, Hayase S (2004) Enhanced enantioselectivity and remarkable acceleration on the lipase-catalyzed transesterification using novel ionic liquids. Green Chem 6:437–439CrossRef Itoh T, Han SH, Matsushita Y, Hayase S (2004) Enhanced enantioselectivity and remarkable acceleration on the lipase-catalyzed transesterification using novel ionic liquids. Green Chem 6:437–439CrossRef
5.
Zurück zum Zitat Moniruzzaman M, Kamiya N, Goto M (2010) Activation and stabilization of enzymes in ionic liquids. Org Biomol Chem 8:2887–2899CrossRef Moniruzzaman M, Kamiya N, Goto M (2010) Activation and stabilization of enzymes in ionic liquids. Org Biomol Chem 8:2887–2899CrossRef
6.
Zurück zum Zitat Fujita K, MacFarlane DR, Forsyth M (2005) Protein solubilising and stabilising ionic liquids. Chem Commun 38:4804–4806CrossRef Fujita K, MacFarlane DR, Forsyth M (2005) Protein solubilising and stabilising ionic liquids. Chem Commun 38:4804–4806CrossRef
7.
Zurück zum Zitat Oppermann S, Stein F, Kragl U (2011) Ionic liquids for two-phase systems and their application for purification, extraction and biocatalysis. Appl Microbiol Biotechnol 89:493–499CrossRef Oppermann S, Stein F, Kragl U (2011) Ionic liquids for two-phase systems and their application for purification, extraction and biocatalysis. Appl Microbiol Biotechnol 89:493–499CrossRef
8.
Zurück zum Zitat Kumar A, Bisht M, Venkatesu P (2017) Biocompatibility of ionic liquids towards protein stability: a comprehensive overview on the current understanding and their implications. Int J Biol Macromol 96:611–651CrossRef Kumar A, Bisht M, Venkatesu P (2017) Biocompatibility of ionic liquids towards protein stability: a comprehensive overview on the current understanding and their implications. Int J Biol Macromol 96:611–651CrossRef
9.
Zurück zum Zitat Sivapragasam M, Moniruzzaman M, Goto M (2016) Recent advances in exploiting ionic liquids for biomolecules: solubility, stability and applications. Biotechnol J 11:1000–1013CrossRef Sivapragasam M, Moniruzzaman M, Goto M (2016) Recent advances in exploiting ionic liquids for biomolecules: solubility, stability and applications. Biotechnol J 11:1000–1013CrossRef
10.
Zurück zum Zitat Ohno H, Suzuki C, Fukumoto K, Yoshizawa M, Fujita K (2003) Electron transfer process of poly(ethylene oxide)-modified cytochrome c in imidazolium type ionic liquid. Chem Lett 32:450–451CrossRef Ohno H, Suzuki C, Fukumoto K, Yoshizawa M, Fujita K (2003) Electron transfer process of poly(ethylene oxide)-modified cytochrome c in imidazolium type ionic liquid. Chem Lett 32:450–451CrossRef
11.
Zurück zum Zitat Shimojo K, Nakashima K, Kamiya N, Goto M (2006) Crown ether-mediated extraction and functional conversion of cytochrome c in ionic liquids. Biomacromolecules 7:2–5CrossRef Shimojo K, Nakashima K, Kamiya N, Goto M (2006) Crown ether-mediated extraction and functional conversion of cytochrome c in ionic liquids. Biomacromolecules 7:2–5CrossRef
12.
Zurück zum Zitat Buchfink R, Tischer A, Patil G, Rudolph R, Lange C (2010) Ionic liquids as refolding additives: variation of the anion. J Biotechnol 150:64–72CrossRef Buchfink R, Tischer A, Patil G, Rudolph R, Lange C (2010) Ionic liquids as refolding additives: variation of the anion. J Biotechnol 150:64–72CrossRef
13.
Zurück zum Zitat Lara C, Reynolds NP, Berryman JT, Xu A, Zhang A, Mezzenga R (2014) ILQINS hexapeptide, identified in lysozyme left-handed helical ribbons and nanotubes, forms right-handed helical ribbons and crystals. J Am Chem Soc 136:4732–4739CrossRef Lara C, Reynolds NP, Berryman JT, Xu A, Zhang A, Mezzenga R (2014) ILQINS hexapeptide, identified in lysozyme left-handed helical ribbons and nanotubes, forms right-handed helical ribbons and crystals. J Am Chem Soc 136:4732–4739CrossRef
14.
Zurück zum Zitat Singh G, Kang TS (2015) Ionic liquid surfactant mediated structural transitions and self-assembly of bovine serum albumin in aqueous media: effect of functionalization of ionic liquid surfactants. J Phys Chem B 119:10573–10585CrossRef Singh G, Kang TS (2015) Ionic liquid surfactant mediated structural transitions and self-assembly of bovine serum albumin in aqueous media: effect of functionalization of ionic liquid surfactants. J Phys Chem B 119:10573–10585CrossRef
15.
Zurück zum Zitat Mangialardo S, Gontrani L, Leonelli F, Caminiti R, Postorino P (2012) Role of ionic liquids in protein refolding: native/fibrillar versus treated lysozyme. RSC Adv 2:12329–12336CrossRef Mangialardo S, Gontrani L, Leonelli F, Caminiti R, Postorino P (2012) Role of ionic liquids in protein refolding: native/fibrillar versus treated lysozyme. RSC Adv 2:12329–12336CrossRef
16.
Zurück zum Zitat Collins KD (2004) Ions from the Hofmeister series and osmolytes: effects on proteins in solution and in the crystallization process. Methods 34:300–311CrossRef Collins KD (2004) Ions from the Hofmeister series and osmolytes: effects on proteins in solution and in the crystallization process. Methods 34:300–311CrossRef
17.
Zurück zum Zitat Kohyama K, Matsumoto T, Imoto T (2010) Refolding of an unstable lysozyme by gradient removal of a solubilizer and gradient addition of a stabilizer. J Biochem 147:427–431CrossRef Kohyama K, Matsumoto T, Imoto T (2010) Refolding of an unstable lysozyme by gradient removal of a solubilizer and gradient addition of a stabilizer. J Biochem 147:427–431CrossRef
18.
Zurück zum Zitat Yamaguchi S, Yamamoto E, Mannen T, Nagamune T, Nagamune T (2013) Protein refolding using chemical refolding additives. Biotechnol J 8:17–31CrossRef Yamaguchi S, Yamamoto E, Mannen T, Nagamune T, Nagamune T (2013) Protein refolding using chemical refolding additives. Biotechnol J 8:17–31CrossRef
19.
Zurück zum Zitat Attri P, Venkatesu P, Kumar A (2012) Water and a protic ionic liquid acted as refolding additives for chemically denatured enzymes. Org Biomol Chem 10:7475–7478CrossRef Attri P, Venkatesu P, Kumar A (2012) Water and a protic ionic liquid acted as refolding additives for chemically denatured enzymes. Org Biomol Chem 10:7475–7478CrossRef
20.
Zurück zum Zitat Bae SW, Eom D, Mai NL, Koo YM (2016) Refolding of horseradish peroxidase is enhanced in presence of metal cofactors and ionic liquids. Biotechnol J 11:464–472CrossRef Bae SW, Eom D, Mai NL, Koo YM (2016) Refolding of horseradish peroxidase is enhanced in presence of metal cofactors and ionic liquids. Biotechnol J 11:464–472CrossRef
21.
Zurück zum Zitat Bisht M, Kumar A, Venkatesu P (2016) Refolding effects of partially immiscible ammonium-based ionic liquids on the urea-induced unfolded lysozyme structure. Phys Chem Chem Phys 18:12419–12422CrossRef Bisht M, Kumar A, Venkatesu P (2016) Refolding effects of partially immiscible ammonium-based ionic liquids on the urea-induced unfolded lysozyme structure. Phys Chem Chem Phys 18:12419–12422CrossRef
22.
Zurück zum Zitat Ghosh S, Dey S, Patel M, Chakrabarti R (2017) Can an ammonium-based room temperature ionic liquid counteract the urea-induced denaturation of a small peptide? Phys Chem Chem Phys 19:7772–7787CrossRef Ghosh S, Dey S, Patel M, Chakrabarti R (2017) Can an ammonium-based room temperature ionic liquid counteract the urea-induced denaturation of a small peptide? Phys Chem Chem Phys 19:7772–7787CrossRef
23.
Zurück zum Zitat Middelberg AP (2002) Preparative protein refolding. Trends Biotechnol 20:437–443CrossRef Middelberg AP (2002) Preparative protein refolding. Trends Biotechnol 20:437–443CrossRef
24.
Zurück zum Zitat Summers CA, Flowers 2nd. RA (2000) Protein renaturation by the liquid organic salt ethylammonium nitrate. Protein Sci 9:2001–2008CrossRef Summers CA, Flowers 2nd. RA (2000) Protein renaturation by the liquid organic salt ethylammonium nitrate. Protein Sci 9:2001–2008CrossRef
25.
Zurück zum Zitat Lange C, Patil G, Rudolph R (2005) Ionic liquids as refolding additives: N′-alkyl and N′-(omega-hydroxyalkyl) N-methylimidazolium chlorides. Protein Sci 14:2693–2701CrossRef Lange C, Patil G, Rudolph R (2005) Ionic liquids as refolding additives: N′-alkyl and N′-(omega-hydroxyalkyl) N-methylimidazolium chlorides. Protein Sci 14:2693–2701CrossRef
26.
Zurück zum Zitat Yamamoto E, Yamaguchi S, Nagamune T (2011) Protein refolding by N-alkylpyridinium and N-alkyl-N-methylpyrrolidinium ionic liquids. Appl Biochem Biotechnol 164:957–967CrossRef Yamamoto E, Yamaguchi S, Nagamune T (2011) Protein refolding by N-alkylpyridinium and N-alkyl-N-methylpyrrolidinium ionic liquids. Appl Biochem Biotechnol 164:957–967CrossRef
27.
Zurück zum Zitat Bharmoria P, Rao KS, Trivedi TJ, Kumar A (2014) Biamphiphilic ionic liquid induced folding alterations in the structure of bovine serum albumin in aqueous medium. J Phys Chem B 118:115–124CrossRef Bharmoria P, Rao KS, Trivedi TJ, Kumar A (2014) Biamphiphilic ionic liquid induced folding alterations in the structure of bovine serum albumin in aqueous medium. J Phys Chem B 118:115–124CrossRef
28.
Zurück zum Zitat Bharmoria P, Mehta MJ, Pancha I, Kumar A (2014) Structural and functional stability of cellulase in aqueous-biamphiphilic ionic liquid surfactant solution. J Phys Chem B 118:9890–9899CrossRef Bharmoria P, Mehta MJ, Pancha I, Kumar A (2014) Structural and functional stability of cellulase in aqueous-biamphiphilic ionic liquid surfactant solution. J Phys Chem B 118:9890–9899CrossRef
29.
Zurück zum Zitat Ohno H, Fujita K, Kohno Y (2015) Is seven the minimum number of water molecules per ion pair for assured biological activity in ionic liquid-water mixtures? Phys Chem Chem Phys 17:14454–14460CrossRef Ohno H, Fujita K, Kohno Y (2015) Is seven the minimum number of water molecules per ion pair for assured biological activity in ionic liquid-water mixtures? Phys Chem Chem Phys 17:14454–14460CrossRef
30.
Zurück zum Zitat Fujita K, MacFarlane DR, Forsyth M, Yoshizawa-Fujita M, Murata K, Nakamura N, Ohno H (2007) Solubility and stability of cytochrome c in hydrated ionic liquids: effect of oxo acid residues and kosmotropicity. Biomacromolecules 8:2080–2086CrossRef Fujita K, MacFarlane DR, Forsyth M, Yoshizawa-Fujita M, Murata K, Nakamura N, Ohno H (2007) Solubility and stability of cytochrome c in hydrated ionic liquids: effect of oxo acid residues and kosmotropicity. Biomacromolecules 8:2080–2086CrossRef
31.
Zurück zum Zitat Fujita K, Ohno H (2012) Stable G-quadruplex structure in a hydrated ion pair: cholinium cation and dihydrogen phosphate anion. Chem Commun 48:5751–5753CrossRef Fujita K, Ohno H (2012) Stable G-quadruplex structure in a hydrated ion pair: cholinium cation and dihydrogen phosphate anion. Chem Commun 48:5751–5753CrossRef
32.
Zurück zum Zitat Fujita K, Kajiyama M, Liu Y, Nakamura N, Ohno H (2016) Hydrated ionic liquids as a liquid chaperon for refolding of aggregated recombinant protein expressed in Escherichia coli. Chem Commun 52:13491–13494CrossRef Fujita K, Kajiyama M, Liu Y, Nakamura N, Ohno H (2016) Hydrated ionic liquids as a liquid chaperon for refolding of aggregated recombinant protein expressed in Escherichia coli. Chem Commun 52:13491–13494CrossRef
33.
Zurück zum Zitat Zhao H (2016) Protein stabilization and enzyme activation in ionic liquids: specific ion effects. J Chem Technol Biotechnol 91:25–50CrossRef Zhao H (2016) Protein stabilization and enzyme activation in ionic liquids: specific ion effects. J Chem Technol Biotechnol 91:25–50CrossRef
Metadaten
Titel
Ionic Liquids as Stabilization and Refolding Additives and Solvents for Proteins
verfasst von
Kyoko Fujita
Copyright-Jahr
2019
DOI
https://doi.org/10.1007/10_2018_65

    Marktübersichten

    Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen.