Abstract
Each normal organ and pathological condition expresses a distinct set of molecules on their vasculature. These molecular signatures have been efficiently profiled using in vivo phage display. Several peptides homing to tumor blood vessels, lymphatic vessels, and/or tumor cells as well as to various normal organs have been isolated using this method. The in vivo screening of phage libraries has also revealed novel tissue-specific biomarkers of the normal and diseased vasculature. Tumor-homing peptides have been successfully used to target therapeutics and imaging agents to tumors. In vivo phage display has also been used in the identification of cell and/or tumor type-specific cell-penetrating peptides, which further facilitate the transmembrane delivery of various cargo molecules into cells. In this review we describe experimental setup for a combined ex vivo and in vivo screening procedure to select both conventional and cell-penetrating peptides homing to brain tumors.
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Acknowledgements
This study was financially supported by the Finnish Cancer Organizations and Sigrid Juselius Foundation. Maija Hyvönen has been supported by the Doctoral Program in Biomedicine (DPBM).
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Hyvönen, M., Laakkonen, P. (2015). Identification and Characterization of Homing Peptides Using In Vivo Peptide Phage Display. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 1324. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2806-4_14
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DOI: https://doi.org/10.1007/978-1-4939-2806-4_14
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2805-7
Online ISBN: 978-1-4939-2806-4
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