Abstract
Analytical methods for probing plant metabolism are taking on new significance in the era of functional genomics, metabolomics, and systems biology. Nuclear magnetic resonance (NMR) is becoming a key technology in plant metabolomics. Stable isotope labeling of cultured cells and higher organisms has been especially promising in that it allows the use of advanced heteronuclear NMR methodologies through a combination of in vivo and in vitro measurements. This new approach provides much better resolution of the metabolite mixture signals in the multidimensional NMR spectra than does the conventional one-dimensional 1H-NMR previously used in plant metabolomics. In this chapter, we describe the practical aspects of two key NMR technologies: uniform stable labeling of plants and in vitro heteronuclear NMR.
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Kikuchi, J., Hirayama, T. (2007). Practical Aspects of Uniform Stable Isotope Labeling of Higher Plants for Heteronuclear NMR-Based Metabolomics. In: Weckwerth, W. (eds) Metabolomics. Methods in Molecular Biology™, vol 358. Humana Press. https://doi.org/10.1007/978-1-59745-244-1_15
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DOI: https://doi.org/10.1007/978-1-59745-244-1_15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-561-3
Online ISBN: 978-1-59745-244-1
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