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Protocols for In Vitro Propagation of Ornamental Plants pp 349–364Cite as

Azalea Phylogeny Reconstructed by Means of Molecular Techniques

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 589))

Abstract

Plants belonging to the Rhododendron subgenera Pentanthera (deciduous) and Tsutsusi and Azaleastrum (evergreen) are called azaleas. Concerning their mutual phylogenetic positions, the Pentanthera subgenus is closer to evergreen rhododendrons (subgenera Rhododendron and Hymenanthes) than to the Tsutsusi subgenus. Both azalea types are important ornamentals with a long breeding tradition. Different hybrid groups are often named after the supposed principal ancestor species. Molecular techniques for phylogenetic and kinship research have been evaluated to a great extent. First, some studies using comparative gene sequencing are presented; this approach was then widened to the use of molecular markers to reveal more detailed genetic relationships. Finally, the use of candidate genes as functional markers for the assessment of genetic diversity is presented. This opens new research lines to the genetic mapping of plant traits and azalea genomics.

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Acknowledgments

The authors wish to thank Romain Uytterhaegen for his hands-on experience in azalea breeding and cultivation, Veerle Buysens, Veerle Cools and Laurence Desmet for their efforts in doing DNA preparations and AFLP, STMS and EST-reactions. The whole staff of the biotech lab was very much appreciated for their support and skilful assistance.

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Authors and Affiliations

  1. Plant Unit, Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research (ILVO), Melle, Belgium

    Ellen De Keyser & Jan De Riek

  2. Department of Agronomy, Forest and Land Management, University of Turin, Grugliasco, Italy

    Valentina Scariot

  3. Laboratory of Plant Breeding, Faculty of Life and Environmental Science, Shimane University, Nishikawatsu, Matsue, Shimane, Japan

    Nobuo Kobayashi

  4. Laboratory of Ornamental Plants, School of Agriculture, Meiji University, Higashi-mita, Tama-ku, Kawasaki, Japan

    Takashi Handa

Authors
  1. Ellen De Keyser
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  2. Valentina Scariot
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  3. Nobuo Kobayashi
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  4. Takashi Handa
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  5. Jan De Riek
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Corresponding author

Correspondence to Ellen De Keyser .

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Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. UMRLEG, PCMV, INRA, Dijon CX, 21065, France

    Sergio J. Ochatt

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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De Keyser, E., Scariot, V., Kobayashi, N., Handa, T., De Riek, J. (2010). Azalea Phylogeny Reconstructed by Means of Molecular Techniques. In: Jain, S., Ochatt, S. (eds) Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology, vol 589. Humana Press. https://doi.org/10.1007/978-1-60327-114-1_30

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  • DOI: https://doi.org/10.1007/978-1-60327-114-1_30

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-390-9

  • Online ISBN: 978-1-60327-114-1

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