Skip to main content

Lime Pretreatment

  • Protocol
  • First Online:
Biofuels

Part of the book series: Methods in Molecular Biology ((MIMB,volume 581))

Summary

Lime pretreatment has proven to be a useful method for selectively reducing the lignin content of lignocellulosic biomass without significant loss in carbohydrates, thus realizing an important increase in biodigestibility. In lime pretreatment, the biomass is pretreated with calcium hydroxide and water under different conditions of temperature and pressure. It can be accomplished in one of three fashions: (1) short-term pretreatment that lasts up to 6 h, requires temperatures of 100–160°C, and can be applied with or without oxygen (pressure ~200 psig); (2) long-term pretreatment taking up to 8 weeks, requiring only 55–65°C, and capable of running with or without air (atmospheric pressure); and (3) simple pretreatment requiring 1 h in boiling water, without air or oxygen. Nonoxidative conditions are effective at low lignin contents (below ~18% lignin), whereas oxidative conditions are required for high lignin contents (above ~18% lignin).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y.Y., Holtzapple, M., Ladish, M., (2005). Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technology 96, 673−686

    Google Scholar 

  2. Sun, Y., Cheng, J. (2002), Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresource Technology 83, 1–11

    Article  CAS  Google Scholar 

  3. O’Dwyer, J., Zhu, L., Granda, C.B., Holtzapple, M.T. (2007). Enzymatic hydrolysis of lime-pretreated corn stover and investigation of the HCH-1 Model: Inhibition pattern, degree of inhibition, validity of simplified HCH-1 Model. Bioresource Technology 98, 2969–2977

    Article  Google Scholar 

  4. Sehoon, K., Holtzapple, M.T. (2006). Effect of structural features on enzyme digestibility of corn stover. Bioresource Technology 97, 583–591

    Article  Google Scholar 

  5. Chang, V.S., Holtzapple, M.T. (2000). Fundamental factors affecting Biomass Enzymatic Reactivity. Applied Biochemistry and Biotechnology 84–86, 1–37

    Google Scholar 

  6. Sehoon, K., Holtzapple, M.T. (2005). Lime pretreatment and enzymatic hydrolysis of corn stover. Bioresource Technology 96, 1994–2006

    Article  Google Scholar 

  7. Chang, V.S., Nagwani, M., Holtzapple, M.T. (1997). Lime pretreatment of switchgrass. Applied Biochemistry and Biotechnology 63–65, 3–19

    Article  Google Scholar 

  8. Chang, V.S., Nagwani, M., Kim, C.H., Holtzapple, M.T. (2001). Oxidative lime pretreatment of high-lignin biomass. Applied Biochemistry and Biotechnology 94, 1–28

    Article  CAS  Google Scholar 

  9. Kaar W.E., Holtzapple M.T. (2000). Using lime pretreatment to facilitate the enzyme hydrolysis of corn stover. Biomass and Bioenergy 18(3), 189−199

    Article  Google Scholar 

  10. Granda, C.B. (2004). Sugarcane Juice Extraction and Preservation, and Long-Term Lime Pretreatment of Bagasse. PhD dissertation. Texas A&M University, College Station, TX

    Google Scholar 

  11. Sierra, R. (2005). Long-Term Lime Pretreatment of Poplar Wood. Thesis. Texas A&M University. College Station, TX

    Google Scholar 

  12. Holtzapple, M.T, Davison, R.R. (1999). Methods of biomass pretreatment. U.S. Patent number 5,865,898

    Google Scholar 

  13. Holtzapple, M.T, Davison, R.R., Nargwani, M. (1997). Calcium Hydroxide pretreatment of biomass. U.S. Patent number 5,693,296

    Google Scholar 

  14. Sjöström E. (1991). Carbohydrate degradation products from alkaline treatment of biomass. Biomass and Bioenergy 1(1), 61–64

    Article  Google Scholar 

  15. Montgomery, R. (1953). Observation of alkaline degradation sugars. Industrial and Engineering Chemistry 45(5), 1144−1147

    Article  CAS  Google Scholar 

  16. Williams, A.G., Morrison, I.M. (1982). Studies on the production of saccharinic acids by the alkaline treatment of young grass and their effectiveness as substrates for mixed rumen microorganisms in vitro. Journal of the Science of Food and Agriculture 33, 21–29

    Article  CAS  Google Scholar 

  17. McGinnis, G.D., Prince, S.E., Biermann, C.J., Lowrimore J.T. (1984). Wet oxidation of modelcarbohydrate compounds. Carbohydrate Research 128, 51–60

    Article  CAS  Google Scholar 

  18. Klinke, H.L., Ahring, B.K., Schmidt, A.S, Thomsen, A.B. (2002). Characterization of degradation products from alkaline wet oxidation of wheat straw. Bioresource Technology 82, 15–26

    Article  CAS  Google Scholar 

  19. DeGroot B.D, van Dam J.E.G., van derZwan R.P., van’t Riet K. (1994). Simplified kinetic modeling of alkaline delignification of hemp woody core. Holzforschung 48(3), 207–214

    Article  CAS  Google Scholar 

  20. Dolk M., Yan, J.F., McCarthy, J.L. (1989). Lignin 25. Kinetics of delignification of western Hemlock in flow-through reactors under alkaline conditions. Holzforschung 43(2), 91–98

    Article  CAS  Google Scholar 

  21. Sehoon, K., Holtzapple, M.T. (2006). Delignification kinetics of corn stover in lime pretreatment. Bioresource Technology 97, 778–785

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Sierra, R., Granda, C.B., Holtzapple, M.T. (2009). Lime Pretreatment. In: Mielenz, J. (eds) Biofuels. Methods in Molecular Biology, vol 581. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-214-8_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-60761-214-8_9

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-213-1

  • Online ISBN: 978-1-60761-214-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics