Abstract
Cellulose is an important biopolymer primarily stored as plant cell wall material. Plant-synthesized cellulose forms elementary fibrils that are micrometers in length and 3–5 nm in dimensions. Cellulose is a dynamic structure, and its size and property vary in different cellulose-containing materials. Atomic force microscopy offers the capability of imaging surface structure at the subnanometer resolution and under nearly physiological conditions, therefore providing an ideal tool for cellulose characterization.
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Himmel ME, Ding S-Y, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315:804–807
Yarbrough JM, Himmel ME, Ding S-Y (2009) Plant cell wall characterization using scanning probe microscopy techniques. Biotechnol Biofuels 2:17
Ding S-Y, Xu Q, Crowley M, Zeng Y, Nimlos M, Lamed R, Bayer EA, Himmel ME (2008) A biophysical perspective on the cellulosome: new opportunities for biomass conversion. Curr Opin Biotechnol 19:218–227
Morris VJ, Kirby AR, Gunning AP (1999) Atomic force microscopy for biologists. Imperial College Press, London
Ding S-Y, Himmel ME (2008) Anatomy and ultrastructure of maize cell walls: an example of energy plants. In: Himmel ME (ed) Biomass recalcitrance. Blackwell, Oxford, pp 38–60
Ding S-Y, Himmel ME (2006) The maize primary cell wall microfibril: a new model derived from direct visualization. J Agric Food Chem 54:597–606
Liu YS, Baker JO, Zeng YN, Himmel ME, Haas T, Ding S-Y (2011) Cellobiohydrolase hydrolyzes crystalline cellulose on hydrophobic faces. J Biol Chem 286:11195–11201
Acknowledgments
This work was supported by the BioEnergy Science Center (BESC), which is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science, Oak Ridge National Laboratory.
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Ding, SY., Liu, YS. (2012). Imaging Cellulose Using Atomic Force Microscopy. In: Himmel, M. (eds) Biomass Conversion. Methods in Molecular Biology, vol 908. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-956-3_3
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DOI: https://doi.org/10.1007/978-1-61779-956-3_3
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