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On-Chip Diagnosis of Celiac Disease by an Amorphous Silicon Chemiluminescence Detector

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Book cover Sensors and Microsystems

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 268))

Abstract

A lab-on-chip for the diagnosis of celiac disease relying on the monitoring of patient-specific immune response to gliadin fractions has been developed. The detection is based on a chemiluminescent immunoenzymatic reaction that ensures high specificity and sensitivity. The chemiluminescent signal is monitored by hydrogenated amorphous silicon photosensors, fabricated on the same glass substrate hosting the biochemical recognition. The main challenge of the work has been the identification of the materials and the setup of the entire process that permitted the reliable fabrication of the device. Experiments performed with serum samples of rabbit immunized towards an epitope show a good specificity of the proposed technique, proving the feasibility of an integrated device for the patient-specific profiling.

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Author information

Authors and Affiliations

  1. Department of Information, Electronic and Telecommunication Engineering, Sapienza University of Rome, Via Eudossiana 18, 00184, Rome, Italy

    D. Caputo, G. de Cesare, R. Scipinotti & N. Stasio

  2. Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy

    F. Costantini & C. Manetti

  3. Department of Astronautics, Electrical and Energy Engineering, Sapienza University of Rome, Via Salaria 851/881, 00138, Rome, Italy

    A. Nascetti

Authors
  1. D. Caputo
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  2. G. de Cesare
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  3. R. Scipinotti
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  4. N. Stasio
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  5. F. Costantini
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  6. C. Manetti
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  7. A. Nascetti
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Corresponding author

Correspondence to R. Scipinotti .

Editor information

Editors and Affiliations

  1. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  2. Department of Information Engineering, University of Brescia, Brescia, Italy

    Vittorio Ferrari

  3. SENSOR Laboratory, IDASC, National Research Council (CNR), Brescia, Italy

    Andrea Ponzoni

  4. Department of Information Engineering, University of Brescia, Brescia, Italy

    Giorgio Sberveglieri

  5. Department of Information Engineering, University of Brescia, Brescia, Italy

    Marco Ferrari

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© 2014 Springer International Publishing Switzerland

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Caputo, D. et al. (2014). On-Chip Diagnosis of Celiac Disease by an Amorphous Silicon Chemiluminescence Detector. In: Di Natale, C., Ferrari, V., Ponzoni, A., Sberveglieri, G., Ferrari, M. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 268. Springer, Cham. https://doi.org/10.1007/978-3-319-00684-0_35

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  • DOI: https://doi.org/10.1007/978-3-319-00684-0_35

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-00683-3

  • Online ISBN: 978-3-319-00684-0

  • eBook Packages: EngineeringEngineering (R0)

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