Abstract
Although natural pigments are in demand, large scale applications of these pigments are restricted due to high pigment production cost. The present study demonstrates on the feasibility of utilizing liquid pineapple waste as low cost growth medium for violet pigment production by Chromobacterium violaceum UTM5 in 5 L bioreactor. Additional supplementation of l-tryptophan was added to increase the yield of pigment. A high pigment yield of 5790 ± 10 mg L−1 was obtained from the cultivation. The yield was 16 times higher than yield obtained from shake flask cultivation. The active compounds were isolated, purified, and characterized as violacein and deoxyviolacein by using FT-IR and LC-MS. Thus, liquid pineapple waste exhibited as a potential low cost growth medium for violet pigment production by C. violaceum UTM5 with an addition of l-tryptophan as supplementation in enhancing the pigment yield.
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Ahmad, W.A., Venil, C.K., Aruldass, C.A. (2015). Production of Violacein by Chromobacterium violaceum Grown in Liquid Pineapple Waste: Current Scenario. In: Liong, MT. (eds) Beneficial Microorganisms in Agriculture, Aquaculture and Other Areas. Microbiology Monographs, vol 29. Springer, Cham. https://doi.org/10.1007/978-3-319-23183-9_3
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DOI: https://doi.org/10.1007/978-3-319-23183-9_3
Publisher Name: Springer, Cham
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