Microplastics (<5 mm particles) occur within both engineered and natural freshwater ecosystems, including wastewater treatment plants, lakes, rivers, and estuaries. While a significant proportion of microplastic pollution is likely sequestered within freshwater environments, these habitats also constitute an important conduit of microscopic polymer particles to oceans worldwide. The quantity of aquatic microplastic waste is predicted to dramatically increase over the next decade, but the fate and biological implications of this pollution are still poorly understood. A growing body of research has aimed to characterize the formation, composition, and spatiotemporal distribution of microplastic-associated (“plastisphere”) microbial biofilms. Plastisphere microorganisms have been suggested to play significant roles in pathogen transfer, modulation of particle buoyancy, and biodegradation of plastic polymers and co-contaminants, yet investigation of these topics within freshwater environments is at a very early stage. Here, what is known about marine plastisphere assemblages is systematically compared with up-to-date findings from freshwater habitats. Through analysis of key differences and likely commonalities between environments, we discuss how an integrated view of these fields of research will enhance our knowledge of the complex behavior and ecological impacts of microplastic pollutants.
1 Introduction
Microplastics (particles with an upper size limit of <5 mm) are globally distributed within aquatic environments, with up to 51 trillion pieces estimated to float at sea alone [1, 2]. They are encountered within the water column and sediments, with the latter functioning as a sink for the accumulation of plastic waste [3‐5]. Most plastic litter originates from land-based activities, with wastewater treatment plant (WWTP) and inland waters comprising an important route through which this pollution reaches marine environments [6, 7]. While a substantial proportion of microplastic is likely to become sequestered within freshwaters, the amount of plastic entering the sea is predicted to increase by an order of magnitude by 2025 (corresponding to an input of up to 250 million metric tons) [7]. Legislation for phasing out microplastics in cosmetic products (e.g., the Microbead-Free Waters Act of 2015 in the USA) can be expected to achieve only a limited reduction in the quantity of environmental plastic debris.
A growing body of research has investigated the impacts of microplastics on biota, which may involve direct and indirect processes (e.g., physical blockage caused by ingested particles, as well as their ability to transport harmful compounds, pathogens, and algae) [2, 8‐10]. Even so, little is known about the ecological effects of microplastics within freshwaters [10]. For example, while microplastic-associated microbial (bacterial, archaeal, and picoeukaryotic) assemblages are likely to profoundly influence the distribution, impacts, and fate of these pollutants, research into this topic has focused on marine environments [11‐13]. In streams and other habitats, biofilms1 are primary sites for carbon and nutrient transformations and form the base of food webs, contributing to local and global ecosystem functioning [14]. As they are also essential to pollutant biodegradation, an improved knowledge of microbial-microplastic interactions is required to predict the environmental impacts of plastic debris [15]. Investigating this topic could inform the development of solutions to manage plastic pollution by determining how it affects processes including microbially mediated primary production and interactions between plastic-associated (“plastisphere”) taxa and other organisms [11, 12, 16, 17]. It could also lead to insights concerning the biodegradability of plastic litter and facilitate the development of new approaches to plastic disposal and/or recycling [18].
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Freshwater and marine habitats share a number of features, but there are also differences between them that may affect the development and activities of plastisphere consortia. To facilitate investigation of this topic, findings based on marine plastisphere research are compared with those available for freshwaters. Following an assessment of recent discoveries concerning the formation and distribution of plastic-associated biofilms, our knowledge concerning their ecological roles and ability to drive processes including polymer biodegradation is considered. Finally, some of the main knowledge gaps in plastisphere research are discussed and used to highlight methodological advances in microbial ecology that could be used to improve our understanding of microbial-microplastic interactions.
2 Freshwater Plastisphere Assemblages: State of the Science
2.1 Factors Contributing to Biofilm Formation and Composition
Fundamental processes involved in biofilm formation are well established, with initial attachment followed by maturation and the eventual detachment of cells [19]. There are also further factors that may influence the formation, composition, and activities of plastic-associated biofilms (Fig. 1). Only some of the parameters shown in Fig. 1 have been investigated with reference to microplastics. However, efforts to identify factors driving the formation of these assemblages in marine habitats have recently been reviewed [12, 13, 20].
Fig. 1
Physical, chemical, and biological factors likely to affect the formation and composition of plastisphere microbial assemblages. Only a limited selection of these parameters has been investigated with specific reference to microplastics
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Microplastics are rapidly colonized by environmental microorganisms (within hours; [21]). Many factors driving the development of plastisphere communities are likely to be similar between freshwater and marine habitats. For example, in agreement with research into biofilm formation on other artificial substrata [19, 22], there is evidence for the importance of surface properties (including roughness and hydrophobicity) during early colonization of microplastics [12, 23]. Exposure to ultraviolet (UV) radiation and waves can modify the surface chemistry and structure of plastics (e.g., via the formation of cracks and pits, a reduction in molecular weight, and an increase in surface oxidation), which may facilitate biofilm formation [24, 25]. Plastic-colonizing microorganisms have also been found to influence the surface properties and buoyancy of polymers [12, 20, 26]. Since microplastics are likely to be transported into marine environments via WWTP, rivers, and streams [6, 7], factors contributing to initial colonization (such as surface roughness and attachment by pioneering colonizers) can be hypothesized to be particularly important within freshwaters. The impacts of particle age and/or weathering on plastisphere consortia may be comparatively pronounced within marine ecosystems where the residence times of plastic often exceed those within rivers and streams [24]. However, microplastics additionally accumulate within environments such as lakes, where they may persist for decades (similar to timescales predicted for marine habitats) and can be exposed to high levels of UV radiation [2, 27, 28]. Local-scale differences in the composition of plastisphere assemblages between polymer types have been found [12, 29, 30], but it is unknown whether there are any general differences in the dominant types of plastic within freshwater and marine ecosystems. Moreover, although it is possible that the ingestion of plastics by higher organisms could have an impact on plastisphere colonization processes, this topic has not been investigated [11, 20, 30].
Ambient conditions such as temperature, salinity, pressure, and the availabilities of light and oxygen are likely to influence the development of plastic-associated biofilms (Fig. 1) [29, 31]. Many of these conditions differ between freshwater and marine ecosystems, and WWTP and unmanaged freshwaters. For example, the low temperatures (<5°C), absence of light, and elevated pressure within deep waters are likely to impose selective forces on plastisphere assemblages that differ from those within shallow habitats. In contrast with the frequently nutrient-poor conditions present within the open ocean, inland and coastal waters receive high fluxes of nutrients from the surrounding environment [14]. In addition to contributions from organic matter input and upwelling, high concentrations of nutrients (e.g., nitrogen and phosphorus) are released by agriculture and other human activities. Many plastisphere members have been affiliated with pollutant degradation [12, 13, 20, 21], and it is probable that several contaminants play a role in shaping biofilm formation and activities on polymers (Fig. 1). Indeed, multiple types of pollutants, as well as heavy metals, are known to become adsorbed onto microplastics [2, 8, 10].
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Further to these factors, physical processes contributing to the movement of suspended particles differ between freshwater and marine habitats [2]. Continuous downstream movement of water is a key distinction between freshwater and marine ecosystems. In rivers, sediment movement is characterized using the concept of “spiraling” [32, 33]. The components of one spiral include downstream transport, deposition, bed load transport, and resuspension. This concept is a well-developed approach for modeling particle movement and is quantified using measurements of deposition length and velocity, turnover time, and the retention-export ratio [34]. To date, direct measurements of spiraling metrics have not been applied to microplastic (but see Kowalski et al. [26], Long et al. [35], and Nizzetto et al. [36]). Each step in a spiral is likely to have implications for plastic-associated biofilm composition and activity, due to accompanying shifts in the surrounding environmental conditions (Fig. 1) [29, 31]. Studies of microplastic spiraling metrics will help estimate the spatial scales over which plastic particles move within lotic environments, informing how the associated microbial communities can be expected to change across multiple downstream spirals. Rivers are also characterized by flooding, which redistributes materials between riparian and aquatic components of the fluvial landscape [37, 38]. Flooding moves plastic from the riparian zone into aquatic habitats and increases stranding of plastic in debris dams [39]. Analogous processes in marine environments include tidal movements and storm surges which strand plastic on intertidal or wrack zones [2]. Despite their likely impacts on plastisphere communities (Fig. 1), the effects of movement between aquatic and terrestrial habitats on plastic-associated biofilms have not been studied.
Hydrology in most lakes includes at least a single upstream inlet and downstream outlet, with water and particle residence times depending on water volume and currents. Little is known about plastisphere communities in lakes (Sect. 2.2), but research into this topic can be expected to benefit from a budgetary approach which measures rates of microplastic inflow, outflow, and retention. These metrics will determine microplastic residence times, which are likely to influence microbial-plastic associations within several habitats, including the epilimnion, littoral, and benthic zones (Sect. 3.1). Wind and wave action are likely to further influence the distribution of microplastics within lakes [2].
It is unclear how transport of microplastics from freshwater to marine environments affects plastisphere assemblages, but they may undergo a variety of taxonomic and physiological shifts during this transition (Sects. 2.2 and 2.3) [20, 40]. For example, subjecting Pseudomonas aeruginosa to salt stress (0.5 M NaCl) was found to inhibit biofilm formation and reduce rates of benzoate degradation by this strain [41]. Geographic and seasonal differences in the structure and composition of freshwater plastisphere communities are yet to be investigated. However, the spatiotemporal distribution of marine plastic-colonizing microbial consortia has recently been studied [29, 30, 42]. Based on 6-week in situ exposures of polyethylene terephthalate (PET) bottles in the North Sea, Oberbeckmann et al. [29, 42] found location-dependent and seasonal differences in the structure and composition of plastisphere communities. Similar differences were also reported by Amaral-Zettler et al. [30]. Further to distinct communities being discovered in the North Atlantic and North Pacific subtropical gyres, the authors reported latitudinal gradients in the species richness of plastic-colonizing assemblages [30]. While taxonomic differences were also observed between polymer types, the data suggested that geography is likely to be a stronger predictor of plastisphere community composition at the scale of ocean basins [29, 30, 42].
2.2 Examples of Microbial-Microplastic Interactions in Freshwater Habitats
Despite measurements of plastic density and composition in freshwater ecosystems [10, 43], little is known about microbial associations with plastic in unmanaged freshwaters. A limited number of publications have investigated polymer biodegradation in lakes and rivers (Sect. 2.3), and there are at least three studies that have experimentally characterized the structure, composition, and/or activities of plastic-associated biofilms in these environments [44‐46]. Because of differences in the study design and sites and the response parameters that were examined, there are few findings in common among these three studies. Thus, some of the major results of each study are discussed and compared with insights into marine microbial-microplastic interactions.
Hoellein et al. [44] compared bacterial community composition and activity on six substrate types (5 × 5 cm pieces of ceramic tile, glass, aluminum, PET, leaf litter, and cardboard) in a river, a pond, and recirculating laboratory streams. In contrast with McCormick et al. [45] and several studies of marine plastisphere communities [21, 29, 47], the authors found no differences in the composition of plastic-colonizing biofilms relative to those on other solid substrates. The plastic, tile, and glass samples also showed similar rates of gross primary production and respiration. The primary factors for determining bacterial community composition and metabolic rates were the study site (river, pond, or artificial stream) and whether the substrate was hard (tile, glass, aluminum, and PET) or soft (leaf litter and cardboard). While the surface-colonizing assemblages on PET were compositionally similar to those on other surfaces, it was suggested that differences between substrate types may be stronger during early stages of biofilm formation. Similarly, Oberbeckmann et al. [42] found PET- and glass-colonizing communities to be compositionally similar following up to 6 weeks of exposure to seawater; the authors noted that higher-resolution studies may be required to distinguish “plastic-specific” taxa from other biofilm members. Taken together, these studies emphasize how investigating the early-stage development of plastisphere communities in more detail will be necessary not only in marine ecosystems [21] but also in freshwater habitats.
McCormick et al. [45] compared bacterial communities on microplastic, suspended organic matter (i.e., seston) and the water column downstream and upstream of a WWTP. All habitats differed from each other, and the microplastic community had a lower taxon diversity relative to seston and downstream water samples. In marine environments, plastic-associated microbial communities have also been found to be taxonomically distinct from those in the surrounding water [30, 47‐49]. Genera selected for on plastic (relative to nonplastic habitats) in the study by McCormick et al. [45] included Pseudomonas, Arcobacter, Aeromonas, Zymophilus, and Aquabacterium. These genera contain species with the potential for plastic degradation and pathogenesis (Sect. 2.3). Aquabacterium commune is a common member of drinking water biofilms [50], and colonization of low-density polyethylene (LDPE) by Arcobacter spp. has also been shown to occur in coastal marine sediments [21]. The study by McCormick et al. [45] was conducted immediately below a WWTP outfall, and it is unknown if wastewater-affiliated microbial communities will persist further downstream. However, the presence of plastic-colonizing Arcobacter spp. in both freshwater and marine habitats [21, 45] implies that certain genera could survive on polymers as they are transported from WWTP to other ecosystems (Fig. 2 and Sect. 2.1). Indeed, Arcobacter spp. have been found to be prevalent members of the “landfill microbiome” in the USA [51] and have also been detected in sewage [52].
Fig. 2
Scanning electron micrograph showing a biofilm attached to a HDPE fragment incubated in aerobic wastewater for 6 months. Microplastics are likely to function as vectors for the transport of microbial taxa from WWTP to other environments. The scale bar is 2 μm (Credit: Alexander S. Tagg)
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The objective of Lagarde et al. [46] was to examine the growth of a microalga (Chlamydomonas reinhardtii) on plastic particles over time, determine the effect of plastic type on algal growth, and measure particle aggregation. The authors found little effect of plastic (high-density polyethylene [HDPE] or polypropylene [PP]) on algal growth, but contact with polymer particles altered the expression of genes for some sugars used in extracellular polysaccharides. On PP, algal biofilms increased particle aggregation, which was not observed for HDPE. Research has recently been aimed at characterizing the sedimentation rates of microplastics in freshwater and marine environments [26, 35, 36]. Lagarde et al. [46] add to our understanding of microplastic movement by showing that aggregation of plastic particles via biofilm attachment occurs differently among polymer types, which will affect their suspension or deposition. Future studies will benefit from extrapolating this approach to in situ analyses, as well as comparing findings between marine and freshwater environments. For example, the types and sinking rates of algal microplastic aggregates within marine environments are known to be species specific [35], and similar interactions could affect the distribution of microplastics in rivers and lakes.
2.3 Potential for Pathogenesis, Toxicant Transfer, and Biodegradation
2.3.1 Microplastics as Vectors for Pathogen Transfer and Biotoxins
Gene sequencing analyses initially highlighted how microplastics may function as vectors for the transport of potential pathogens including Vibrio and Arcobacter spp. [21, 30, 45, 48, 53]. A high proportion of 16S rDNA reads (24%) could be attributed to Vibrio spp. detected on PP and, to a lesser extent, on polyethylene (PE) collected at a station in North Atlantic waters [48]. Unfortunately, the widely used bacterial metabarcoding technique based on sequencing fragments of the 16S rRNA gene is limited in its ability to provide the required taxonomic resolution for detecting human pathogens [53]. Using oligotyping of 16S rRNA gene data, Schmidt et al. [54] obtained more specific results for taxa within the genus Vibrio indicating the presence of potential pathogens affecting animals including fishes, corals, and bivalves in marine or mixed saline plastic samples. The presence of pathogens on plastics sampled from seawater was also implied by increased abundances of genes involved in type IV and type VI secretion systems [49]. However, genes involved in these systems can be involved not only in virulence and infection [55] but also in conjugation [56] and interbacterial interactions [57] that are important in biofilms [58]. Vibrio spp. were additionally isolated from plastic collected from a Scottish beach [59], but no further characterization of the isolates was performed. Only recently was the presence of Vibrio spp. on marine plastics conclusively confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) [60]. In their study, Kirstein et al. [60] identified V. parahaemolyticus, V. fluviales, and V. alginolyticus on microplastics from the North Sea. Apart from V. alginolyticus, these species were also found on plastics collected in the brackish Baltic Sea. In addition to bacteria, microplastics may transport microbial eukaryotes involved in disease transmission [12]. Potentially harmful algae, including Ostreopsis and Coolia spp., have been discovered on plastic in the Mediterranean Sea [61]. To date, the only in situ evidence for microplastic-associated pathogens in unmanaged freshwaters identified an increase in Campylobacteraceae attached to microplastics sourced from an urban river [45]. Specifically, 16S rRNA gene sequences related to Arcobacter and Pseudomonas spp. were enriched on plastic in comparison with other suspended matter and the surrounding water.
In summary, current evidence indicates an important role of microplastics as vectors for opportunistic animal and human pathogens. Methodological advances are required to reliably detect viable pathogenic species, so that realistic distribution patterns can be obtained and potential sources can be identified. This is particularly relevant with regard to waters used for recreational [13] but also for industrial purposes such as aquaculture. Relative abundances of Aeromonas spp. (a genus harboring fish pathogens) were increased on riverine plastics [45], implying that such species could take advantage of microplastics as vectors. This possibility is reinforced by the presence of Aeromonas salmonicida, causing furunculosis in hatcheries, on several plastic types [62]. Recently, 16S rRNA gene sequences affiliated to Tenacibaculum spp. (another genus including fish pathogens) were detected on PET in seawater [42]. Research has only started to shed light on this issue, as well as the ability of polymers to transport biologically produced toxins.
2.3.2 Biodegradation and Pollutant Transport
Several reviews of research into plastic biodegradation have been published (e.g., see [11‐13, 24, 63‐65]). Therefore, only a brief overview of this topic is provided. Plastic biodegradation involves several steps during which the polymer is enzymatically cleaved into oligomers and monomers that can be assimilated by microorganisms [65]. Many microbial taxa can degrade biopolymers2 including polyhydroxybutyrate (PHB) and polyhydroxybutyrate-polyhydroxyvalerate (PHBV). The biodegradation rates of biopolymers in freshwater have been found to exceed those in marine environments, and higher rates have also been observed in sewage than within natural freshwaters [63, 66, 67]. Even so, these materials can still persist for considerable periods of time in freshwaters, with a lifespan of ~10 years having been estimated for PHBV bottles deposited onto lake sediments at a depth of 85 m [68].
In comparison with biopolymers, traditional plastics (such as PE, PET, and PP) will persist for even longer within aquatic environments (decades or centuries; [11, 63, 64]), with biodegradation typically preceded by abiotic weathering [24, 65]. Although it has been unclear whether plastisphere members can biodegrade conventional plastics [11, 69, 70], a bacterial strain isolated from sediment near a Japanese bottle recycling facility (Ideonella sakaiensis) was recently found to assimilate PET [18]. The strain was shown to employ two enzymes to degrade PET at a daily rate of 0.13 mg cm−2 when incubated at 30°C [18]. This finding implies that other synthetic plastic-degrading taxa are likely to be present within aquatic environments. Indeed, colonization of plastics by potentially hydrocarbonoclastic bacteria has been observed in both marine and freshwater habitats [21, 45, 47‐49]. However, due to a lack of research into plastisphere physiology, the long residence times of plastic waste, and the ability of polymers to adsorb polyaromatic hydrocarbons [11, 12], the mechanisms underlying recruitment of hydrocarbon degraders on microplastics are unknown. These and other taxa could mediate desorption and/or degradation of several plastic-associated compounds, including additives and diverse pollutants, with implications for the ecological impacts of microplastics. Indeed, Bryant et al. [49] already reported the presence of diverse xenobiotic degradation genes in association with marine plastic debris. Since organic contaminants and metals rapidly partition into biofilms [71, 72], plastisphere communities may alternatively be hypothesized to facilitate transport of pollutants between ecosystems and to biota (Sect. 3.2).
3 Knowledge Gaps and Research Needs
3.1 Sources and Transport Between Habitats
Processes contributing to microplastic transport differ between freshwater and marine ecosystems (Sect. 2.1). Conditions encountered within WWTP and unmanaged freshwaters also differ from one another. A priority for research involves determining the extent to which plastic-colonizing taxa associated with wastewater and other sources of plastic (such as landfills) are transported downstream along rivers and streams and whether they remain viable and active upon entering marine habitats [12, 40]. As part of this work, research is required to characterize the residence times of polymer particles within several environments, including different stages of the wastewater treatment process. Most WWTPs are based on three main treatment stages, although slight differences in their configuration can be found. During primary treatment, large debris fragments are removed by using a 6 mm (or larger) screen mesh. During secondary treatment, large aeration tanks are used to remove suspended and dissolved organic material and nutrients through microbial activity. Subsequently, flocculates and settling tanks are used to facilitate separation of sewage sludge from the post-processing effluent prior to a potential disinfection step, also known as advanced tertiary treatment. Studies reporting pathways of microplastics through different wastewater treatment stages are only beginning to emerge [73‐75], and little is still known about how these stages influence the development of plastisphere microbial communities.
Overall, studies of microplastic movement and associated biofilms should be based on well-established principles of ecosystem and community ecology [39] and are prerequisite to estimating the spatial scales over which plastics are distributed within a watershed. This approach will best inform how plastic-associated microbial communities can be expected to change with movement from freshwater to marine habitats. There is also a need to compare plastisphere communities in managed and natural environments, within several locations along the water column, as well as between pelagic and benthic habitats. Research into plastic-associated biofilms has focused on surface waters (despite the long-term accumulation of microplastics in sediments; [8, 27]), and investigations of benthic plastisphere assemblages have been restricted to marine habitats [21, 47]. In several environments, no information is available on plastic-associated microbial assemblages. For example, no data have yet been published on plastisphere consortia within WWTP, and although the buildup of plastic debris in deep-sea environments has been reported [76], biofilms associated with this debris have not been studied. This lack of data limits our ability to predict the ecological consequences and lifetimes of plastic pollution (Sects. 3.2 and 3.3).
3.2 Interactions with Higher Organisms and the Wider Environment
Interactions between plastisphere communities and higher organisms have been recommended as a topic for research in marine environments [11, 12], but they also require investigation within freshwaters. Many organisms including fishes, gastropods, and zooplankton (e.g., Daphnia magna) ingest microplastics [2]. Indeed, nanopolystyrene has been found to negatively affect reproduction in D. magna, as well as population growth in the primary producer Scenedesmus obliquus [77]. Effects of plastic-sorbed chemicals have been rarely studied, but liver toxicity was observed in Japanese medaka [78]. A significant knowledge gap is the in situ analysis of microplastic present within freshwater organisms. Such analyses will need to consider how plastic-associated biofilms may amend the buoyancy of polymer particles and/or influence organismal behavior (e.g., selective feeding). Additionally, research is needed to investigate the pathogenicity of plastic-colonizing microbial taxa, as well as their ability to produce toxins. Oberbeckmann et al. [12] suggested that microplastics could carry pathogens encountered in the feces of marine organisms, and transport of human fecal bacteria on plastics has also been discussed [13]. There is a particular requirement to determine how this debris affects organisms at low trophic levels, such as invertebrates used for biomonitoring purposes [79, 80]. Impacts of plastisphere assemblages on processes such as nutrient cycling and primary production should also be investigated. Indeed, Bryant et al. [49] reported high densities of chlorophyll a and an increased abundance of nitrogen fixation genes (nifH, nifD, and nifK) on polymers in comparison with other sample types, leading the authors to suggest that plastic particles may constitute autotrophic “hot spots” in seawater.
Further to impacts on the fitness of plastic-ingesting taxa and processes including elemental cycling, interactions between plastisphere assemblages and other organisms may influence the distribution and fate of plastic waste. For example, microplastics may become transported away from surface waters via encapsulation within fecal pellets [81]. Although this topic has not been investigated in freshwater or marine environments, the gut bacteria of mealworms (larvae of Tenebrio molitor Linnaeus) can degrade polystyrene [82], and certain aquatic organisms could harbor microorganisms capable of modifying the surface properties of plastics and/or biodegrading them. Thus, investigating the interactions between plastisphere communities and other organisms is closely connected to research into the transport of plastics between habitats (Sect. 3.1) and the environmental lifetime of this debris (Sect. 3.3).
While this chapter focuses on freshwater and marine environments, plastisphere communities may also be of significance to human health. Risks associated with the human ingestion of microscopic plastics have been identified [83], and investigations of this topic could also be approached from a microbiological viewpoint. In particular, the human health implications of putative pathogens within plastic-associated biofilms (Sect. 2.3.1 and [13]) merit further study.
3.3 In Situ Biodegradability of Plastics and Plastic-Associated Compounds
The recent evidence for PET assimilation by I. sakaiensis [18] suggests that, although rates of plastic breakdown in the environment are extremely low (Sect. 2.3.2), several novel polymer-degrading taxa are likely to be present within freshwater and marine ecosystems. Identifying such taxa and investigating their ability to biodegrade different plastic types, additives, and polymer-sorbed compounds are of primary importance to understanding the environmental residence times of plastic waste. Research in this area should focus on habitats functioning as sinks for the accumulation of plastic, including sediments [3‐5, 27]. To obtain a complete understanding of the biodegradability of different materials and compounds, there is a need to combine laboratory-based experiments with field-based measurements of plastic degradation in both freshwater and marine environments. Moreover, as nanometer-sized plastic particles become released from the parent polymer as a result of weathering [84], their biodegradation behavior will need to be compared with that of larger fragments that may support a comparatively complex biofilm community. Most research into plastic biodegradation has been based on indirect measurements such as mass loss [11], and a key challenge will be to conclusively demonstrate in situ assimilation of carbon from a given plastic type (or plastic-associated compound) [18]. The toxicity of any degradation products, or of compounds released from the polymer, will also require investigation (Sect. 3.2).
3.4 Analytical and Experimental Advances in Plastisphere Research
Research into plastisphere assemblages has focused on bacterial communities [44, 45]. Little is known about plastic-associated microbial eukaryotes in freshwaters, and there is a need for analyses targeting these organisms, not the least as they are known to occur on marine plastics [48, 49]. Several advances have improved the suitability of metabarcoding for analyzing fungi, diatoms, and protists [85‐87]. This approach is based on taxonomically informative markers and provides no direct information on metabolic activities. Overcoming this limitation could involve using metagenomics or metatranscriptomics, with the former providing information on metabolic capability [49] and the latter enabling investigations of functional gene expression [20] (Fig. 3). The origin of plastic-colonizing pathogens could be determined by whole genome sequencing followed by genome comparisons or identification of single-nucleotide polymorphism (SNP), approaches widely used in bacterial epidemiology. This would result in important insights into the transfer of pathogens on plastics, provided that suitable databases are available for comparison [88, 89].
Fig. 3
Moving beyond initial research into the taxonomy and formation of plastisphere microbial assemblages. As investigations of this topic mature, new types of experiments and analytical tools are anticipated to improve our knowledge of topics including how plastisphere communities develop in several types of habitat, how they are affected by transport from freshwater to marine environments, and the metabolic functions of plastic-colonizing microorganisms
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Several further developments could enable us to move beyond initial studies of biofilm formation on microplastics (Fig. 3). Stable isotope labeling is increasingly used to characterize microbial activity at the single-cell level, including methods such as heavy water labeling [90] and bioorthogonal noncanonical amino acid tagging (BONCAT) [91]. Heavy water labeling is compatible with Raman spectroscopy and cell sorting using optical tweezers [90], and BONCAT has been combined with fluorescence-activated cell sorting (FACS) [91]. These approaches could be followed by multiple displacement amplification (MDA)3, enabling identification of taxa that are metabolically active under in situ conditions. Raman spectroscopy has been combined with techniques such as fluorescence in situ hybridization (FISH), which can be used to further investigate the presence and activities of specific microbial taxa [92]. Fourier-transform infrared (FT-IR) spectroscopy has additionally been employed to characterize the chemical composition of biofilms, providing a convenient and low-cost method for analyzing microorganisms adhering to opaque materials [93]. Such methods could be used in conjunction with biological rate measurements (e.g., gas evolution) [44, 49]. This, in turn, could advance our understanding of how plastisphere taxa contribute to disease transmission, nutrient fixation, and pollutant degradation.
Research into microplastic-associated biofilms has relied on samples that were collected in situ or exposed to seawater, with only a small selection of studies involving microcosm experiments under controlled conditions [21, 46, 59]. Mesocosm experiments could be used to bridge the current gap between microcosm studies and field-based research into microplastic-associated biofilms (Fig. 3). Microfluidics is also increasingly used as a tool in microbial ecology and could be employed to obtain insights into microbial-microplastic interactions under selected conditions (e.g., in the presence of fluid flow and chemical gradients) [94, 95]. To improve our knowledge of the biodegradation of plastics and plastic-sorbed pollutants, such approaches could be supplemented by advanced surface analysis techniques. X-ray photoelectron spectroscopy (XPS) and secondary ion mass spectrometry (SIMS) have been used to investigate abiotic weathering of plastics [96‐98] and could be valuable for monitoring polymer biodegradation (Fig. 3). Indeed, XPS can detect chemical signatures at the parts-per-thousand (‰) range [96], and SIMS (including nanoscale SIMS) has been used to trace microbial uptake of 13C-labeled substrates in environmental samples [99, 100]. While these techniques are suitable for analyzing organic compounds, X-ray diffraction (XRD) analyses are particularly useful for measurements of inorganic materials, including metals. Although microbial interactions with plastic-associated metals (e.g., metal solubilization or precipitation) have not been previously studied, this could be achieved using XRD (e.g., see Roh et al. [101]).
4 Concluding Remarks
Over the past 5 years, several studies have improved our understanding of the taxonomy and potential activities of microbial consortia associated with microplastic particles in the environment. Due to most of these studies focusing on marine ecosystems, there remains a particular lack of information concerning plastisphere assemblages within freshwaters. However, as highlighted in this chapter, many of the fundamental processes that underpin the formation and activities of plastic-colonizing biofilms remain poorly understood within both freshwater and marine environments. Establishing an understanding of the implications of microplastic-associated microorganisms for ecosystem and human health, therefore, will require research spanning the entire diversity of environments encountered by these pollutants following their release by industrial and domestic activities.
Acknowledgments
We thank Buck Hanson, Toby Samuels, and William Southwell-Wright for their feedback and helpful suggestions.
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