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Metabolic Engineering

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Book cover Genetic Modification of Plants

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 64))

Abstract

The metabolic engineering of primary metabolism provides an enormous potential to improve the value of plant-based raw materials for food and industrial applications. This chapter focuses on the current progress in the manipulation of carbohydrate and lipid biosynthesis in transgenic starch and oilseed crops, respectively. In most approaches, the manipulation of the biosynthetic routes revealed limitations and bottlenecks in the pathways, and we discuss how the gain of knowledge led to improved strategies to circumvent these restrictions. We summarize recent advances in improving starch yield as well as starch quality, i.e. the ratio of amylopectin to amylose, for particular industrial uses, and we present the strategies to produce novel beneficial carbohydrates in crops. We also describe the numerous approaches to obtain high value plant oil by the targeting of unusual fatty acids into the seed oil of oilseed crops by introducing biosynthetic enzymes of exotic species in order to improve the suitability of the engineered vegetable oil as renewable resource for biofuel production, as chemical feedstock and for food and feed. Finally, we briefly discuss recent successful examples to engineer plant secondary metabolism to enhance plant nutritional value

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References

  • Agius F, Gonzalez-Lamothe R, Caballero JL, Munoz-Blanco J, Botella MA, Valpuesta V (2003) Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase. Nat Biotechnol 21:177–181

    Article  PubMed  CAS  Google Scholar 

  • Al-Babili S, Beyer P (2005) Golden Rice -- five years on the road -- five years to go? Trends Plant Sci 10:565–573

    Article  PubMed  CAS  Google Scholar 

  • Altmann T, Felix G, Jessop A, Kauschmann A, Uwer U, Penacortes H, Willmitzer L (1995) Ac/Ds transposon mutagenesis in Arabidopsis thaliana -- mutant spectrum and frequency of Ds insertion mutants. Mol Gen Genet 247:646–652

    Article  PubMed  CAS  Google Scholar 

  • An S, Park S, Jeong D-H, Lee D-Y, Kang H-G, Yu J-H, Hur J, Kim S-R, Kim Y-H, Lee M, Han S, Kim S-J, Yang J, Kim E, Wi SJ, Chung HS, Hong J-P, Choe V, Lee H-K, Choi J-H, Nam J, Kim S-R, Park P-B, Park KY, Kim WT, Choe S, Lee C-B, An G (2003) Generation and analysis of end sequence database for T-DNA tagging lines in rice. Plant Physiol 133:2040–2047

    Article  PubMed  CAS  Google Scholar 

  • ap Rees T, Hill SA (1994) Metabolic control analysis of plant metabolism. Plant Cell Environ 17:587–599

    Article  Google Scholar 

  • Bailey JE (1991) Toward a science of metabolic engineering. Science 252:1668–1675

    Article  PubMed  CAS  Google Scholar 

  • Bates PD, Ohlrogge JB, Pollard M (2007) Incorporation of newly synthesized fatty acids into cytosolic glycerolipids in pea leaves occurs via acyl editing. J Biol Chem 282:31206–31216

    Article  PubMed  CAS  Google Scholar 

  • Beyer P, Al-Babili S, Ye XD, Lucca P, Schaub P, Welsch R, Potrykus I (2002) Golden rice: Introducing the beta-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin A deficiency. J Nutr 132:506S–510S

    PubMed  Google Scholar 

  • Börnke F, Hajirezaei M, Sonnewald U (2001) Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici. J Bacteriol 183:2425–2430

    Article  PubMed  Google Scholar 

  • Börnke F, Hajirezaei M, Heineke D, Melzer M, Herbers K, Sonnewald U (2002) High-level production of the non-cariogenic sucrose isomer palatinose in transgenic tobacco plants strongly impairs development. Planta 214:356–364

    Article  PubMed  Google Scholar 

  • Broun P (2004) Transcription factors as tools for metabolic engineering in plants. Curr Opin Plant Biol 7:202–209

    Article  PubMed  CAS  Google Scholar 

  • Broun P, Somerville C (1997) Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean. Plant Physiol 113:933–942

    Article  PubMed  CAS  Google Scholar 

  • Butelli E, Titta L, Giorgio M, Mock HP, Matros A, Peterek S, Schijlen EG, Hall RD, Bovy AG, Luo J, Martin C (2008) Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol 26:1301–1308

    Article  PubMed  CAS  Google Scholar 

  • Cahoon EB, Ohlrogge JB (1994) Apparent role of phosphatidylcholine in the metabolism of petroselinic acid in developing Umbelliferae endosperm. Plant Physiol 104:845–855

    PubMed  CAS  Google Scholar 

  • Cahoon EB, Carlson TJ, Ripp KG, Schweiger BJ, Cook GA, Hall SE, Kinney AJ (1999) Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos. Proc Natl Acad Sci USA 96:12935–12940

    Article  PubMed  CAS  Google Scholar 

  • Cahoon EB, Hall SE, Ripp KG, Ganzke TS, Hitz WD, Coughlan SJ (2003) Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. Nat Biotechnol 21:1082–1087

    Article  PubMed  CAS  Google Scholar 

  • Cahoon EB, Dietrich CR, Meyer K, Damude HG, Dyer JM, Kinney AJ (2006) Conjugated fatty acids accumulate to high levels in phospholipids of metabolically engineered soybean and Arabidopsis seeds. Phytochem 67:1166–1176

    Article  CAS  Google Scholar 

  • Cahoon EB, Shockey JM, Dietrich CR, Gidda SK, Mullen RT, Dyer JM (2007) Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux. Curr Opin Plant Biol 10:236–244

    Article  PubMed  CAS  Google Scholar 

  • Dahlqvist A, Stahl U, Lenman M, Banas A, Lee M, Sandager L, Ronne H, Stymne S (2000) Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci USA 97:6487–6492

    Article  PubMed  CAS  Google Scholar 

  • Davuluri GR, van Tuinen A, Fraser PD, Manfredonia A, Newman R, Burgess D, Brummell DA, King SR, Palys J, Uhlig J, Bramley PM, Pennings HMJ, Bowler C (2005) Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat Biotechnol 23:890–895

    Article  PubMed  CAS  Google Scholar 

  • Dehesh K (2001) How can we genetically engineer oilseed crops to produce high levels of medium-chain fatty acids? Eur J Lipid Sci Technol103:688–697

    Article  CAS  Google Scholar 

  • DellaPenna D, Last RL (2006) Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol Plant 126:356–368

    Article  CAS  Google Scholar 

  • DellaPenna D, Pogson BJ (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annu Rev Plant Biol 57:711–738

    Article  PubMed  CAS  Google Scholar 

  • Durrett TP, Benning C, Ohlrogge J (2008) Plant triacylglycerols as feedstocks for the production of biofuels. Plant J 54:593–607

    Article  PubMed  CAS  Google Scholar 

  • Dyer JM, Chapital DC, Kuan JW, Mullen RT, Pepperman AB (2002) Metabolic engineering of Saccharomyces cerevisiae for production of novel lipid compounds. Appl Microbiol Biotechnol 59:224–230

    Article  PubMed  CAS  Google Scholar 

  • Dyer JM, Stymne S, Green AG, Carlsson AS (2008) High-value oils from plants. Plant J 54: 640–655

    Article  PubMed  CAS  Google Scholar 

  • Fernie AR, Willmitzer L, Trethewey RN (2002) Sucrose to starch: a transition in molecular plant physiology. Trends Plant Sci 7:35–41

    Article  PubMed  CAS  Google Scholar 

  • Geigenberger P (2003) Regulation of sucrose to starch conversion in growing potato tubers. J Exp Bot 54:457–465

    Article  PubMed  CAS  Google Scholar 

  • Geigenberger P, Stamme C, Tjaden J, Schulz A, Quick PW, Betsche T, Kersting HJ, Neuhaus HE (2001) Tuber physiology and properties of starch from tubers of transgenic potato plants with altered plastidic adenylate transporter activity. Plant Physiol 125:1667–1678

    Article  PubMed  CAS  Google Scholar 

  • Geigenberger P, Stitt M, Fernie AR (2004) Metabolic control analysis and regulation of the conversion of sucrose to starch in growing potato tubers. Plant Cell Environ 27:655–673

    Article  CAS  Google Scholar 

  • Grotewold E (2008) Transcription factors for predictive plant metabolic engineering: are we there yet? Curr Opin Biotechnol 19:138–144

    Article  PubMed  CAS  Google Scholar 

  • Hellwege EM, Czapla S, Jahnke A, Willmitzer L, Heyer AG (2000) Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots. Proc Natl Acad Sci USA 97:8699–8704

    Article  PubMed  CAS  Google Scholar 

  • Henikoff S, Till BJ, Comai L (2004) TILLING. Traditional mutagenesis meets functional genomics. Plant Physiol 135:630–636

    Article  PubMed  CAS  Google Scholar 

  • Herbers K (2003) Vitamin production in transgenic plants. J Plant Physiol 160:821–829

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann M, Wagner M, Abbadi A, Fulda M, Feussner I (2008) Metabolic engineering of omega3-very long chain polyunsaturated fatty acid production by an exclusively acyl-CoA-dependent pathway. J Biol Chem 283:22352–22362

    Article  PubMed  CAS  Google Scholar 

  • Jacobsen JR, Khosla C (1998) New directions in metabolic engineering. Curr Opin Chem Biol 2:133–137

    Article  PubMed  CAS  Google Scholar 

  • Jako C, Kumar A, Wei Y, Zou J, Barton DL, Giblin EM, Covello PS, Taylor DC (2001) Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol 126:861–874

    Article  PubMed  CAS  Google Scholar 

  • Jeon J-S, Lee S, Jung K-H, Jun S-H, Jeong D-H, Lee J, Kim C, Jang S, Lee S, Yang K, Nam J, An K, Han M-J, Sung R-J, Choi H-S, Yu J-H, Choi J-H, Cho S-Y, Cha S-S, Kim S-I, An G (2000) T-DNA insertional mutagenesis for functional genomics in rice. Plant J 22:561–570

    Article  PubMed  CAS  Google Scholar 

  • Jobling S (2004) Improving starch for food and industrial applications. Curr Opin Plant Biol 7:210–218

    Article  PubMed  CAS  Google Scholar 

  • Jobling SA, Jarman C, Teh MM, Holmberg N, Blake C, Verhoeyen ME (2003) Immunomodulation of enzyme function in plants by single-domain antibody fragments. Nat Biotechnol 21:77–80

    Article  PubMed  CAS  Google Scholar 

  • Kammerer B, Fischer K, Hilpert B, Schubert S, Gutensohn M, Weber A, Flügge UI (1998) Molecular characterization of a carbon transporter in plastids from heterotrophic tissues: The glucose 6-phosphate phosphate antiporter. Plant Cell 10:105–117

    PubMed  CAS  Google Scholar 

  • Kim CM, Piao HL, Park SJ, Chon NS, Je BI, Sun B, Park SH, Park JY, Lee EJ, Kim MJ, Chung WS, Lee KH, Lee YS, Lee JJ, Won YJ, Yi G, Nam MH, Cha YS, Yun DW, Eun MY, Han C-d (2004) Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice. Plant J 39:252–263

    Article  PubMed  CAS  Google Scholar 

  • Kinney AJ (2006) Metabolic engineering in plants for human health and nutrition. Curr Opin Biotechnol 17:130–138

    Article  PubMed  CAS  Google Scholar 

  • Knutzon DS, Hayes TR, Wyrick A, Xiong H, Maelor Davies H, Voelker TA (1999) Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels. Plant Physiol 120:739–746

    Article  PubMed  CAS  Google Scholar 

  • Kroon JTM, Wei WX, Simon WJ, Slabas AR (2006) Identification and functional expression of a type 2 acyl-CoA : diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homology to the major triglyceride biosynthetic enzyme of fungi and animals. Phytochemistry 67:2541–2549

    Article  PubMed  CAS  Google Scholar 

  • Larson TR, Edgell T, Byrne J, Dehesh K, Graham IA (2002) Acyl CoA profiles of transgenic plants that accumulate medium-chain fatty acids indicate inefficient storage lipid synthesis in developing oilseeds. Plant J 32:519–527

    Article  PubMed  CAS  Google Scholar 

  • Lee M, Lenman M, Banas A, Bafor M, Singh S, Schweizer M, Nilsson R, Liljenberg C, Dahlqvist A, Gummeson PO, Sjodahl S, Green A, Stymne S (1998) Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation. Science 280:915–918

    Article  PubMed  CAS  Google Scholar 

  • Mansoor S, Amin I, Hussain M, Zafar Y, Briddon RW (2006) Engineering novel traits in plants through RNA interference. Trends Plant Sci 11:559–565

    Article  PubMed  CAS  Google Scholar 

  • Metzger JO, Bornscheuer U (2006) Lipids as renewable resources: current state of chemical and biotechnological conversion and diversification. Appl Microbiol Biotechnol 71:13–22

    Article  PubMed  CAS  Google Scholar 

  • Meyer FD, Talbert LE, Martin JM, Lanning SP, Greene TW, Giroux MJ (2007) Field evaluation of transgenic wheat expressing a modified ADP-glucose pyrophosphorylase large subunit. Crop Sci 47:336–342

    Article  CAS  Google Scholar 

  • Muir SR, Collins GJ, Robinson S, Hughes S, Bovy A, Ric De Vos CH, van Tunen AJ, Verhoeyen ME (2001) Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nat Biotechnol 19:470–474

    Article  PubMed  CAS  Google Scholar 

  • Nath U, Becker HC, Möllers C (2006) Increasing erucic acid content in rapeseed. In: OIRA (ed) Oils, fats and lipids for a healthier future, Madrid. Proc Eur Fed Lipid Congr 4:OIRA-003

    Google Scholar 

  • Ohlrogge J, Browse J (1995) Lipid biosynthesis. Plant Cell 7:957–970

    PubMed  CAS  Google Scholar 

  • Paine JA, Shipton CA, Chaggar S, Howells RM, Kennedy MJ, Vernon G, Wright SY, Hinchliffe E, Adams JL, Silverstone AL, Drake R (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nat Biotechnol 23:482–487

    Article  PubMed  CAS  Google Scholar 

  • Qi B, Fraser T, Mugford S, Dobson G, Sayanova O, Butler J, Napier JA, Stobart AK, Lazarus CM (2004) Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nat Biotechnol 22:739–745

    Article  PubMed  CAS  Google Scholar 

  • Regierer B, Fernie AR, Springer F, Perez-Melis A, Leisse A, Koehl K, Willmitzer L, Geigenberger P, Kossmann J (2002) Starch content and yield increase as a result of altering adenylate pools in transgenic plants. Nat Biotechnol 20:1256–1260

    Article  PubMed  CAS  Google Scholar 

  • Ritsema T, Smeekens SCM (2003) Engineering fructan metabolism in plants. J Plant Physiol 160:811–820

    Article  PubMed  CAS  Google Scholar 

  • Sayanova O, Haslam R, Venegas Caleron M, Napier JA (2007) Cloning and characterization of unusual fatty acid desaturases from Anemone leveillei: identification of an acyl-coenzyme A C20 Delta5-desaturase responsible for the synthesis of sciadonic acid. Plant Physiol 144:455–467

    Article  PubMed  CAS  Google Scholar 

  • Schijlen EGWM, Ric de Vos CH, van Tunen AJ, Bovy AG (2004) Modification of flavonoid biosynthesis in crop plants. Phytochemistry 65:2631–2648

    Article  PubMed  CAS  Google Scholar 

  • Schultz DJ, Suh MC, Ohlrogge JB (2000) Stearoyl-acyl carrier protein and unusual acyl-acyl carrier protein desaturase activities are differentially influenced by ferredoxin. Plant Physiol 124:681–692

    Article  PubMed  CAS  Google Scholar 

  • Sevenier R, Hall RD, van der Meer IM, Hakkert HJ, van Tunen AJ, Koops AJ (1998) High level fructan accumulation in a transgenic sugar beet. Nat Biotechnol 16:843–846

    Article  PubMed  CAS  Google Scholar 

  • Shintani D, DellaPenna D (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282:2098–2100

    Article  PubMed  CAS  Google Scholar 

  • Shockey JM, Gidda SK, Chapital DC, Kuan JC, Dhanoa PK, Bland JM, Rothstein SJ, Mullen RT, Dyer JM (2006) Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 18:2294–2313

    Article  PubMed  CAS  Google Scholar 

  • Smidansky ED, Clancy M, Meyer FD, Lanning SP, Blake NK, Talbert LE, Giroux MJ (2002) Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield. Proc Natl Acad Sci USA 99:1724–1729

    Article  PubMed  CAS  Google Scholar 

  • Smidansky ED, Martin JM, Hannah LC, Fischer AM, Giroux MJ (2003) Seed yield and plant biomass increases in rice are conferred by deregulation of endosperm ADP-glucose pyrophosphorylase. Planta 216:656–664

    PubMed  CAS  Google Scholar 

  • Smidansky ED, Meyer FD, Blakeslee B, Weglarz TE, Greene TW, Giroux MJ (2007) Expression of a modified ADP-glucose pyrophosphorylase large subunit in wheat seeds stimulates photosynthesis and carbon metabolism. Planta 225:965–976

    Article  PubMed  CAS  Google Scholar 

  • Stark DM, Timmerman KP, Barry GF, Preiss J, Kishore GM (1992) Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase. Science 258:287–292

    Article  PubMed  CAS  Google Scholar 

  • Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang GF, Lambert W, Van der Straeten D (2007) Folate fortification of rice by metabolic engineering. Nat Biotechnol 25:1277–1279

    Article  PubMed  CAS  Google Scholar 

  • Suh MC, Schultz DJ, Ohlrogge JB (2002) What limits production of unusual monoenoic fatty acids in transgenic plants? Planta 215:584–595

    Article  PubMed  CAS  Google Scholar 

  • Sweetlove LJ, Burrell MM, ap Rees T (1996) Characterization of transgenic potato (Solanum tuberosum) tubers with increased ADP glucose pyrophosphorylase. Biochem J 320:487–492

    PubMed  CAS  Google Scholar 

  • Tang GL, Galili G, Zhuang X (2007) RNAi and microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering. Metabolomics 3: 357–369

    Article  CAS  Google Scholar 

  • Thomaeus S, Carlsson AS, Stymne S (2001) Distribution of fatty acids in polar and neutral lipids during seed development in Arabidopsis thaliana genetically engineered to produce acetylenic, epoxy and hydroxy fatty acids. Plant Sci 161:997–1003

    Article  CAS  Google Scholar 

  • Tjaden J, Möhlmann T, Kampfenkel K, Henrichs G, Neuhaus HE (1998) Altered plastidic ATP/ADP-transporter activity influences potato (Solanum tuberosum L.) tuber morphology, yield and composition of tuber starch. Plant J 16:531–540

    Article  CAS  Google Scholar 

  • Tomlinson K, Denyer K (2003) Starch synthesis in cereal grains. Adv Bot Res 40:1–61

    Article  CAS  Google Scholar 

  • van de Loo FJ, Broun P, Turner S, Somerville C (1995) An oleate 12-hydroxylase from Ricinus communis l is a fatty acyl desaturase homolog. Proc Natl Acad Sci USA 92:6743–6747

    Article  PubMed  Google Scholar 

  • Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, Jiang J, Baszis SR, Levering CK, Aasen ED, Hao M, Stein JC, Norris SR, Last RL (2003) Engineering vitamin E content: from Arabidopsis mutant to soy oil. Plant Cell 15:3007–3019

    Article  PubMed  Google Scholar 

  • Visser RGF, Somhorst I, Kuipers GJ, Ruys NJ, Feenstra WJ, Jacobsen E (1991) Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol Gen Genet 225:289–296

    Article  PubMed  CAS  Google Scholar 

  • Voelker TA, Worrell AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Radke SE, Davies HM (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257:72–74

    Article  PubMed  CAS  Google Scholar 

  • Weyens G, Ritsema T, Van Dun K, Meyer D, Lommel M, Lathouwers J, Rosquin I, Denys P, Tossens A, Nijs M, Turk S, Gerrits N, Bink S, Walraven B, Lefebvre M, Smeekens S (2004) Production of tailor-made fructans in sugar beet by expression of onion fructosyltransferase genes. Plant Biotechnol J 2:321–327

    Article  PubMed  CAS  Google Scholar 

  • Wiberg E, Edwards P, Byrne J, Stymne S, Dehesh K (2000) The distribution of caprylate, caprate and laurate in lipids from developing and mature seeds of transgenic Brassica napus L. Planta 212:33–40

    Article  PubMed  CAS  Google Scholar 

  • Wu GH, Truksa M, Datla N, Vrinten P, Bauer J, Zank T, Cirpus P, Heinz E, Qiu X (2005) Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nat Biotechnol 23:1013–1017

    Article  PubMed  CAS  Google Scholar 

  • Wu L, Birch RG (2007) Doubled sugar content in sugarcane plants modified to produce a sucrose isomer. Plant Biotechnol J 5:109–117

    Article  PubMed  CAS  Google Scholar 

  • Ye X, Al-Babili S, Ouml KL, Ti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:303–305

    Article  PubMed  CAS  Google Scholar 

  • Yu K, McCracken CT, Jr, Li R, Hildebrand DF (2006) Diacylglycerol acyltransferases from Vernonia and Stokesia prefer substrates with vernolic acid. Lipids 41:557–566

    Article  PubMed  CAS  Google Scholar 

  • Zhang L, Häusler RE, Greiten C, Hajirezaei MR, Haferkamp I, Neuhaus HE, Flügge UI, Ludewig F (2008) Overriding the co-limiting import of carbon and energy into tuber amyloplasts increases the starch content and yield of transgenic potato plants. Plant Biotechnol J 6:453–464

    Article  PubMed  CAS  Google Scholar 

  • Zhu C, Naqvi S, Gomez-Galera S, Pelacho AM, Capell T, Christou P (2007) Transgenic strategies for the nutritional enhancement of plants. Trends Plant Sci 12:548–555

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Lehrstuhl für Biochemie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 5, 91058, Erlangen, Germany

    Lars M. Voll & Frederik Börnke

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  1. Lars M. Voll
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  2. Frederik Börnke
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Correspondence to Lars M. Voll .

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Editors and Affiliations

  1. Botanisches Institut, Universität Kiel, Olshausenstr. 40, Kiel, 24118, Germany

    Frank Kempken

  2. Inst. Pflanzenbau und Pflanzenzüchtung, Universität Kiel, Olshausenstr. 40, Kiel, 24098, Germany

    Christian Jung

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Voll, L.M., Börnke, F. (2010). Metabolic Engineering. In: Kempken, F., Jung, C. (eds) Genetic Modification of Plants. Biotechnology in Agriculture and Forestry, vol 64. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02391-0_11

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