Abstract
Isolated microspore culture (IMC) represents a unique system of single cell reprogramming in plants wherein a haploid male gametophyte, the microspore, switches its default gametophytic developmental pathway toward embryogenesis by specific stress treatment. The application of a stress treatment(s) is necessary for efficient embryogenesis induction. Depending on species, microspores are often induced by cold and heat shock, osmotic stress, starvation, anti-microtubular agents, stress hormones, antibiotics, or polyamines. This technique (IMC) is likely to remain as a well-known method in plant breeding since it allows for the rapid production of completely homozygous lines while, in the context of developmental biology, it allows for in vitro embryogenesis to be explored in greater detail. Isolated microspores also represent ideal recipients for several gene transfer techniques including electroporation, microprojectile bombardment, and Agrobacterium-mediated transformation. IMC is also extensively used for genetic studies, i.e., studying inheritance of quantitative traits, quantitative trait loci (QTL) mapping, and genomics and gene identification, for mutation and selection and also used for producing reversible male-sterile lines. Male sterility avoids the labor costs of manual emasculation and serves as a molecular strategy for transgene containment by preventing pollen release to the environment. Combination of this technique with doubled haploid (DH) production leads to an innovative environmentally friendly breeding technology. In addition, the usefulness of DHs for reverse breeding program, an applied plant breeding technique introduced to directly produce parental lines for any hybrid plant, is also generally discussed.
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References
Abdollahi MR, Corral-Martínez P, Mousavi A, Salmanian AH, Moini A, Seguí-Simarro JM (2009) An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation. Acta Physiol Plant 31(6):1313–1317
Ahloowalia BS, Maluszynski M (2001) Induced mutations-a new paradigm in plant breeding. Euphytica 118:167–173
Ahmad I, Day JP, MacDonald MV, Ingram DS (1991) Haploid culture and UV mutagenesis in rapid-cycling Brassica napus for the generation of resistance to chlorsulfuron and Alternaria brassicicola. Ann Bot 67(6):521–525
Ahmadi B, Alizadeh K, Teixeira da Silva JA (2012a) Enhanced regeneration of haploid plantlets from microspores of Brassica napus L. using bleomycin, PCIB, and phytohormones. Plant Cell Tissue Organ Cult 109:525–533
Ahmadi B, Ghadimzadeh M, Moghaddam AF, Alizadeh K, Teixeira da Silva JA (2012b) Bud length, plating density, and incubation time on microspore embryogenesis in Brassica napus. Int J Veg Sci 18:346–357
Ahmadi B, Shariatpanahi ME, Teixeira da Silva JA (2014) Efficient induction of microspore embryogenesis using abscisic acid, jasmonic acid and salicylic acid in Brassica napus L. Plant Cell Tissue Organ Cult 116:343–351
Ajisaka H, Kuginuki Y, Yui S, Enomoto S, Hirai M (2001) Identification and mapping of a quantitative trait locus controlling extreme late bolting in Chinese cabbage (Brassica rapa L. ssp. Pekinensis syn. campestris L.) using bulked segregant analysis. Euphytica 118:75–81
Asif M (2013) History, production methods, and types of haploids. Prog Oppor Doubled Haploid Plant 6:1–6
Asif M, Eudes F, Goyal A, Amundsen E, Randhawa H, Spaner D (2013a) Organelle antioxidants improve microspore embryogenesis in wheat and triticale. In Vitro Cell Dev Biol Plant 49(5):489–497
Asif M, Eudes F, Randhawa H, Amundsen E, Yanke J, Spaner D (2013b) Cefotaxime prevents microbial contamination and improves microspore embryogenesis in wheat and triticale. Plant Cell Rep 32(10):1637–1646
Ayed OS, Buyser JD, Picard E, Trifa Y, Amara HS (2010) Effect of pre-treatment on isolated microspore culture ability in durum wheat (Triticum turgidum subsp. durum Desf.). J Plant Breed Crop Sci 2(2):30–38
Bal U, Elliatioglu S, Abak K (2009) Induction of symmetrical nucleus division and multi-nucleate structures in microspores of eggplant (Solanum melongena L.) cultured in vitro. Sci Agric 66(4):535–539
Barro F, Fernandez-Escobar J, De la Vega M, Martin A (2001) Doubled haploid lines of Brassica carinata with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores. Plant Breed 120:262–264
Barro F, Fernandez-Escobar J, De la Vega M, Martin A (2002) Modification of glucosinolate and erucic acid contents in doubled haploid lines of Brassica carinata by UV treatment of isolated microspores. Euphytica 129:1–6
Birchler JA, Auger DL, Riddle NC (2003) In search of the molecular basis of heterosis. Plant Cell 15:2236–2239
Bohanec B (2009) Doubled haploids via gynogenesis. In: Touraev A, Forster BP, Jain SM (eds) Advances in haploid production in higher plants. Springer Science+Business Media B.V, Dordrecht, pp 35–46. ISBN 978-1-4020-8853-7
Bourgin JP, Nitsch JP (1967) Obtention de Nicotiana haploids a partir d’etamines cultivees in vitro. Ann Physiol Veg 9:377–382
Brew-Appiah RA, Ankrah N, Liu W, Konzak CF, von Wettstein D, Rustgi S (2013) Generation of doubled haploid transgenic wheat lines by microspore transformation. PLoS One. doi:10.1371/journal.pone.0080155
Cegielska-Taras T, Pniewski T, Szala L (2008) Transformation of microspore-derived embryos of winter oilseed rape (Brassica napus L.) by using Agrobacterium tumefaciens. J Appl Genet 49(4):343–347
Chan J, Pauls KP (2007) Brassica napus Rop GTPases and their expression in microspore cultures. Planta 225:469–484
Chen G, Geng J, Rahman M, Liu X, Tu J, Fu T, Li G, McVetty PBE, Tahir M (2010) Identification of QTL for oil content, seed yield, and flowering time in oilseed rape (Brassica napus). Euphytica 175:161–174
Clapham D, Manders G, Yibrah HS, von Arnold S (1995) Enhancement of short- and medium-term expression of transgenes in embryogenic suspensions of Picea abies (L.) Karst. J Exp Bot 46:655–662
Cloud V, Chan YL, Grubb J, Budke B, Bishop DK (2012) Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis. Science 337(6099):1222–1225
Cloutier S, Ragupathy R, Niu Z, Duguid S (2011) SSR-based linkage map of flax (Linum usitatissimum L.) and mapping of QTLs underlying fatty acid composition traits. Mol Breed 28:437–451
Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philos Trans R Soc B 363:557–572
Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169–196
Dirks R, van Dun K, de Soon CB, van der Berg M, Lelivelt CLC, Voermans W et al (2009) Reverse breeding: a novel breeding approach based on engineered meiosis. Plant Biotechnol J 7:837845
Dubas E, Wedzony M, Petrovska B, Salaj J, Żur I (2010) Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale (x Triticosecale Wittm.). Acta Biol Cracov Bot 52(1):73–86
Dubas E, Custers J, Kieft H, Wedzony M, van Lammeren AA (2011) Microtubule configurations and nuclear DNA synthesis during initiation of suspensor-bearing embryos from Brassica napus cv. Topas microspores. Plant Cell Rep 30(11):2105–2116
Dubas E, Janowiak F, Krzewska M, Hura T, Żur I (2013) Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction. Plant Cell Rep 32(9):1465–1475
Dubas E, Moravčíková J, Libantová J, Matušíková I, Benková E, Żur I, Krzewska M (2014) The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore-derived embryos. Protoplasma 251:1077–1087
Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424
Feng-Ian Z, Takahata Y (1999) Microspore mutagenesis and in vitro selection for resistance to soft rot disease in soft rot in Chinese cabbage (Brassica campestris L. spp. pekinensis). Breed Sci 49:161–166
Fennell A, Hauptmann R (1992) Electroporation and PEG delivery of DNA into maize microspores. Plant Cell Rep 11:567–570
Ferrie AMR, Caswell KL (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Cult 104:301–309
Ferrie AMR, Möllers C (2010) Haploids and doubled haploids in Brassica spp. for genetic and genomic research. Plant Cell Tiss Org Cult 104:375–386
Ferrie AMR, Taylor DC, MacKenzie SL, Rakow G, Raney JP, Keller WA (2008) Microspore mutagenesis of Brassica species for fatty acid modifications: a preliminary evaluation. Plant Breed 127:501–506
Folling L, Olesen A (2001) Transformation of wheat (Triticum aestivum L.) microspore derived callus and microspores by particle bombardment. Plant Cell Rep 20(7):629–636
Forster BP, Thomas WTB (2003) Doubled haploids in genetic mapping and genomic. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Kluwer Academic Publishers, Dordrecht, pp 367–390
Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375
Ge Y, Wang T, Wang N, Wang Z, Liang C, Ramchiary N, Choi SR, Lim YP, Piao ZY (2012) Genetic mapping and localization of quantitative trait loci for chlorophyll content in Chinese cabbage (Brassica rapa ssp. pekinensis). Sci Horticult 147:42–48
Germana MA (2006) Doubled haploid production in fruit crops. Plant Cell Tiss Org Cult 86:131–146
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 42:909–930
Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497
Gurushidze M, Hensel G, Hiekel S, Schedel S, Valkov V et al (2014) True-breeding targeted gene knock-out in barley using designer TALE-nuclease in haploid cells. PLoS One 9(3):e92046. doi:10.1371/journal.pone.0092046
Han SY, Zhang HY, Yang ML, Zhao TJ, Gai JY, Yu DY (2007) Screening of mutants and construction of mutant population in soybean “Nannong 86-4”. Acta Agron Sin 33:2059–2062
Hansel G, Oleszczuk S, Daghma DES, Zimny J, Melzer M, Kumlehn J (2012) Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x Triticosecale Wittmack). BMC Plant Biol 12:171
Hoseini M, Ghadimzadeh M, Ahmadi B, Teixeira da Silva JA (2014) Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis in Brassica napus L. In Vitro Cell Dev Biol Plant 50:26–35
Huang S, Liu Z, Li D, Yao R, Meng Q, Feng H (2014) Screening of Chinese cabbage mutants produced by 60Co γ-ray mutagenesis of isolated microspore cultures. Plant Breed 133:480–488
Hussain B, Khan MA, Ali Q, Shaukat S (2012) Double haploid production is the best method for genetic improvement and genetic studies of wheat. Int J Agro Vet Med Sci 6(4):216–228
Ilyas M, Ilyas N, Arshad M, Kazi AG, Kazi AM, Waheed A (2014) QTL mapping of wheat doubled for chlorophyll content and chlorophyll fluorescence kinetics under drought stress imposed at anthesis stage. Pak J Bot 46(5):1889–1897
Immonen S, Anttila H (1996) Success in rye anther culture. Vortr Pflanzenzüchtg 35:237–244
Indrianto A, Mariani TS, Sari DA (2014) Induction of embryogenic microspore in oil palm (Elaeis guineensis Jacq) by starvation and temperature stress. Asia J Appl Sci 2(5):668–677
Ingram HM, Power JB, Lowe KC, Davey MR (1999) Optimisation of procedures for microprojectile bombardment of microspore-derived embryos in wheat. Plant Cell Tiss Org Cult 57:207–210
Islam SMS (2010) The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.). Aust J Crop Sci 4(9):660–665
Janska A, Zelenkova S, Klima M, Vyvadilova M, Prasil IT (2010) Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape. Czech J Genet Plant Breed 46(1):35–40
Jardinaut MF, Souvre A, Alibert G (1993) Transient GUS gene expression in Brassica napus electroporated microspores. Plant Sci 93:177–184
Jardinaut MF, Souvre A, Beckert M, Alibert G (1995) Optimization of DNA transfer and transient b-glucuronidase expression in electroporated maize (Zea mays L.) microspores. Plant Cell Rep 15:55–58
Jauhar PP (2003) Haploid and doubled haploid production in durum wheat by anther culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: a manual. Kluwer Academic Publishers, Dordrecht, pp 167–172
Jin M, Lee SS, Ke L, Kim JS, Seo MS, Sohn SH, Park BS, Bonnema G (2014) Identification and mapping of a novel dominant resistance gene, TuRB07 to Turnip mosaic virus in Brassica rapa. Theor Appl Genet 127:509–519
Jung KH, An G (2013) Functional characterization of rice genes using a gene-indexed T-DNA insertional mutant population. Rice Protoc 956:57–67
Kim M, Jang IC, Kim JA, Park EJ, Yoon M, Lee Y (2008) Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Rep 27(3):425–434
Klima M, Vyvadilova M, Kucera V (2008) Chromosome doubling effects of selected antimitotic agents in Brassica napus microspore culture. Czech J Genet Plant Breed 44(1):30–36
Lantos C, Juhász AG, Mihály PVR, Kristóf Z, Paul J (2012) Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.). Plant Biotechnol Rep 6(2):123–132
Li JR, Zhuang FY, Qu CG, Hu H, Zhao ZW, Mao JH (2012) Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.). Plant Cell Tiss Org Cult 112(3):275–287
Lionneton E, Ravera S, Sanchez L, Aubert G, Delourme R, Ochatt S (2002) Development of an AFLP-based linkage map and localization of QTLs for seed fatty acid content in condiment mustard (Brassica juncea). Genome 45:1203–1215
Liu S, Wang H, Zhang J, Fitt BDL, Xu Z, Evans N, Liu Y, Yang W, Guo X (2005) In vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotiorum. Plant Cell Rep 24:133–144
Lou P, Zhao J, He H, Hanhart C, Carpio DND, Verkerk R, Custers J, Koorneef M, Bonnema G (2008) Quantitative trait loci for glucosinolate accumulation in Brassica rapa leaves. New Phytol 179:1017–1032
Ma H (2005) Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants. Annu Rev Plant Biol 56:393–434
Martinez V, Thorgaard G, Robison B, Sillanpää MJ (2005) An application of Bayesian QTL mapping to early development in double haploid lines of rainbow trout including environmental effects. Genet Res 86(3):209–221
McClinchey SL, Kott LS (2008) Production of mutants with high cold tolerance in spring canola (Brassica napus). Euphytica 162:51–67
Mentewab A, Letellier V, Marque C, Sarrafi A (1999) Use of anthocyanin biosynthesis stimulatory genes as markers for the genetic transformation of haploid embryos and isolated microspores in wheat. Cereal Res Commun 27(1–2):17–24
Mishra VK, Goswami R (2014) Haploid production in higher plant. Int J Chem Biol Sci 1(1):25–45
Muñoz-Amatriaín M, Sevensson JT, Castillo AM, Cistue L, Close TJ (2006) Transcriptome analysis of barely anthers: effect of mannitol treatment on microspore embryogenesis. Physiol Plant 127:551–560
Nehlin L, Mollers C, Bergman P, Glimelius K (2000) Transient f3-gus and gfp gene expression and viability analysis of microprojectile bombarded microspores of Brassica napus L. J Plant Physiol 156:175–183
Nishioka M, Tamura K, Hayashi M, Fujimori Y, Ohkawa Y, Kuginuki Y, Harada K (2005) Mapping of QTLs for bolting time in Brassica rapa (syn. campestris) under different environmental conditions. Breed Sci 55:127–133
Obert B, Ponya Z, Pretova A, Barnabas B (2004) Optimization of electroporation conditions for maize microspores. Maydica 49:15–19
Palmgren MG, Edenbrandt AK, Elizabeth S, Andersen VMM, Landes X, Østerberg JT, Falhof J et al (2014) Are we ready for back-to-nature crop breeding? Trend Plant Sci 1238:1–10
Parez-Prat E, van Lookeren Compagne MM (2002) Hybrid seed production and the challenge of propagating malesterile plants. Trends Plant Sci 7(5):199–203
Pink D, Bailey L, McClement S, Hand P, Mathas E, Buchanan-Wollaston V, Astley D, King G, Teakle G (2008) Double haploids, markers and QTL analysis in vegetable Brassicas. Euphytica 164:509–514
Polsoni L, Kott LS, Beversdorf WD (1987) Large-scale microspore culture technique for mutation – selection studies in Brassica napus. Can J Bot 66:1681–1685
Portemer V, Renne C, Guillebaux A, Mercier R (2015) Large genetic screens for gynogenesis and androgenesis haploid inducers in Arabidopsis thaliana failed to identify mutants. Front Plant Sci 6:147. doi:10.3389/fpls.2015.00147
Prem D, Solís MT, Bárány I, Rodríguez-Sanz H, Risueño MC, Testillano PS (2012a) A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus. BMC Plant Biol 12:127. doi:10.1186/1471-2229-12-127
Prem D, Gupta K, Agnihotri A (2012b) Harnessing mutant donor plants for microspore culture in Indian mustard [Brassica juncea (L.) Czern and Coss]. Euphytica 184:207–222
Qin YH, Teixeira da Silva JA, Bi JH, Zhang SL, Hu GB (2011) Response of in vitro strawberry to antibiotics. Plant Growth Regul 65:183–193
Resch T, Touraev A (2011) Pollen transformation technologies. In: Stewart N, Touraev A, Citovsky V, Tzfira T, (eds) Plant transformation technologies. Wiley-Blackwell, Chichester, pp 83–91
Ribarits A, Mamun ANK, Li S, Resch T, Fiers M, Heberle-Bors E, Liu CM, Touraev A (2007) Combination of reversible male sterility and doubled haploid production by targeted inactivation of cytoplasmic glutamine synthetase in developing anthers and pollen. Plant Biotechnol J 5:483–494
Rodríguez-Sanz H, Manzanera J, Solis M, Gómez-Garay A, Pintos B, Risueño MC, Testillano PS (2014) Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L. BMC Biol. doi:10.1186/s12870-014-0224-4
Rodríguez-Serrano M, Bárány I, Prem D, Coronado MJ, Risueño MC, Testillano PS (2011) NO, ROS, and cell death associated with caspase-like activity increase in stress-induced microspore embryogenesis of barley. J Exp Bot 63:2007–2024
Rygulla W, Snowdon RJ, Friedt W, Happstadius I, Cheung WY, Chen D (2008) Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus). Genet Res 98(2):215–221
Scofield S, Dewitte W, Nieuwland J, Murray JA (2013) The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity. Plant J 75(1):53–66
Seyis F, Aydin E, Catal MI (2014) Haploids in the improvement of crucifers. Turk J Agric Nat Sci 2:1419–1424
Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plant 127:519–534
Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot. doi:10.1155/2012/217037
Shim YS, Pauls KP, Kasha KJ (2009) Transformation of isolated barely (Hordeum vulgare L.) microspores: II: timing of pretreatment and temperatures relative to results of bombardment. Gemone 52:175–190
Soriano M, Cistue L, Valles MP, Castillo AM (2007) Effects of colchicine on anther and microspore culture of bread wheat (Triticum aestivum L.). Plant Cell Tiss Org Cult 91(3):225–234
Soriano M, Li H, Boutilier K (2013) Microspore embryogenesis: establishment of embryo identity and pattern in culture. Plant Reprod 26:181–196
Stasolla C, Belmonte MF, Tahir M, Elhiti M, Khamiss K, Joosen R, Maliepaard C, Sharpe A, Gjetvaj B, Boutilier K (2008) Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation. Planta 228(2):255–272
Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka H, Matsumoto S, Hirai M (2003) Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor Appl Genet 107:997–1002
Swanson EB, Coumans MP, Brown GL, Patel JD, Beversdorf WD (1988) The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts. Plant Cell Rep 7:83–87
Swanson EB, Herrgesell MJ, Arnoldo M, Sippell DW, Wong RSC (1989) Microspore mutagenesis and selection: canola plants with field tolerance to the imidazolinones. Theor Appl Genet 78:525–530
Szarejko I, Forster BP (2007) Doubled haploidy and induced mutation. Euphytica 130:359–370
Takahata Y, Fukuoka H, Wakui K (2005) Utilization of microspore-derived embryos. In: Plamer CE, Keller WA, Kasha KJ (eds) Haploids in crop improvement II. Springer, Berlin, pp 153–169
Toppino L, Kooiker M, Lindner M, Dreni L, Rotino GL, Kater MM (2011) Reversible male sterility in eggplant (Solanum melongena L.) by artificial microRNA-mediated silencing of general transcription factor genes. Plant Biotechnol J 9:684–692
Travella S, Ross SM, Harden J, Everett C, Snape JW, Harwood WA (2005) A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Rep 23:780–789
Wang NL, Long T, Yao W, Xiong LZ, Zhang QF, Wu CY (2013) Mutant resources for the functional analysis of the rice genome. Mol Plant 6:596–604
Wijnker E, van Dun K, de Snoo CB, Lelivelt CLC, Keurentjes JJB, Naharudin NS, Ravi M, Chan SWL, de Jong H, Dirks R (2012) Reverse breeding in Arabidopsis thaliana generates homozygous parental lines from a heterozygous plant. Nat Genet 44:467–470
Wu J, Cai G, Tu J, Li L, Liu S et al (2013) Identification of QTLs for resistance to Sclerotinia stem rot and BnaC.IGMT5.a as a candidate gene of the major resistant QTL SRC6 in Brassica napus. PLoS One 8(7):e67740. doi:10.1371/journal.pone.0067740
Xu YH, Chen F, Dong ZD, Cui DQ (2010) Construction and analysis of EMS induced mutant library of hexaploid wheat cultivar Yunong 201. J Tritic Crops 30:625–629
Yang X, Yu YJ, Zhang FL, Zou ZR, Zhao XY, Zhang DS, Xu JB (2007) Linkage map construction and quantitative trait loci analysis for bolting based on a double haploid population of Brassica rapa. J Integr Plant Biol 49(5):664–671
Yang P, Shu S, Chen L, Xu J, Wu J, Liu K (2012) Identification of a major QTL for silique length and seed weight in oilseed rape (Brassica napus L.). Theor Appl Genet 125:285–296
Yao QA, Simion E, William M, Krochko J, Kasha KJ (1997) Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.). Genome 40:570–581
Yu F, Lydiate DJ, Rimmer SR (2005) Identification of two novel genes for blackleg resistance in Brassica napus. Theor Appl Genet 110:969–979
Yu F, Lydiate DJ, Rimmer SR (2008) Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris. Genome 51:64–72
Yu S, Zhang F, Yu R, Zou Y, Qi J, Zhao X, Yu Y, Zhang D, Li L (2009) Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis). Mol Breed 23:573–590
Zhang FL, Wang M, Liu XC, Zhao XY, Yang JP (2008) Quantitative trait loci analysis for resistance against Turnip mosaic virus based on a doubled-haploid population in Chinese cabbage. Plant Breed 127:82–86
Ziemienowicz A, Shim Y-S, Matsuoka A, Eudes F, Kovalchuk I (2012) A novel method of transgene delivery into Triticale plants using the Agrobacterium transferred DNA-derived nano-complex. Plant Physiol 158:1503–1513
Żur I, Dubas E, Golemier F, Szechynska-Hebda M, Golehiowska G, Wedzony M (2009) Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (× Triticosecale Wittm.). Plant Cell Rep 28:1279–1287
Żur I, Krzewska M, Dubas E, Gołębiowska-Pikania G, Janowiak F, Stojalowski S (2012) Molecular mapping of loci associated with abscisic acid accumulation in triticale (×Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development. Plant Growth Regul 68(3):483–492
Żur L, Dubas E, Krzewska M, Janowiak F, Hura K, Pociecha E, Baczek-Kwinta R, Plazek A (2014) Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell Tiss Org Cult 119:79–94
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Shariatpanahi, M.E., Ahmadi, B. (2016). Isolated Microspore Culture and Its Applications in Plant Breeding and Genetics. In: Anis, M., Ahmad, N. (eds) Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer, Singapore. https://doi.org/10.1007/978-981-10-1917-3_21
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