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On the presence of organic phosphate in some Camargue sediments: evidence for the importance of phytate

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Abstract

The organic phosphate pool of some Camargue sediments (South of France) was studied, after removal of inorganic phosphate, with Ca-NTA/dithionite (Fe bound phosphate) and Na-EDTA (Ca bound phosphate). The organic phosphate was divided into an acid soluble organic phosphate fraction (ASOP) and a residual organic phosphate fraction (ROP). The extraction of organic matter with 2.0 M NaOH (90 °C) from ROP yielded considerable quantities of Org-P. In this extract the presence of phytate (inositol hexa phosphate) could be demonstrated using phytase to hydrolyse the phytate. Phytate was shown to account for a considerable part of organic phosphate in sediments of freshwater marsh sediments as well as in the sediment of the brackish/salt water lake ‘Etang de Vaccares’. In laboratory experiments phytate was found to precipitate with all poly-valent cations tested. Furthermore, phytate was found to be strongly adsorbed onto Fe(OOH), which may explain its accumulation and its stability in sediments.

Considerable quantities of ASOP were found; the chemical stucture of this pool remains unknown.

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Abbreviations

ASOP:

acid soluble organic phosphate

CaCO3 ≈ P:

calcium bound phosphate

Fe(OOH):

iron hydroxide

Fe(OOH) ≈ P:

iron bound phosphate

NaOHextr-P:

phosphate extracted with NaOH (90 °C) after ASOP extraction.

Phyt-P:

phytate bound phosphate

ROP:

residual organic phosphate

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Authors and Affiliations

  1. Associatition ‘Leiden-Camargue’, Station Biologique de la Tour du Valat, Le Sambuc, 13200, Arles, France

    C. J. De Groot & H. L. Golterman

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  1. C. J. De Groot
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  2. H. L. Golterman
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De Groot, C.J., Golterman, H.L. On the presence of organic phosphate in some Camargue sediments: evidence for the importance of phytate. Hydrobiologia 252, 117–126 (1993). https://doi.org/10.1007/BF00000133

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