Abstract
Cells of the non-N2-fixing cyanobacteriumPhormidium laminosum were immobilized in polyurethane (PU) foams either by absorption or by entrapment in the PU prepolymer followed by polymerisation and by adsorption onto polyvinyl (PV) foams. Although entrapment caused toxicity problems which lead to rapid death of the immobilized cells, they were immobilized successfully by adsorption onto PU or PV foams and maintained their photosynthetic electron transport activities (PS I, II, I + II) for at least 7 weeks. Changes in the morphology resulting from immobilization, as revealed by scanning electron microscopy (SEM) and low temperature-SEM, were investigated. Batch cultures and a continuous-flow packed bed photobioreactor were used to study nitrate removal from water. The effects of light intensity and CO2 concentration on bioreactor performance were studied with respect to the nitrate uptake efficiency of the system. It was concluded thatP. laminosum immobilized on polymer foams is of potential value for biological nitrate removal in a continuous-flow system.
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References
Affolter D, Hall DO (1986) Long-term stability of photosynthetic electron transport in polyvinyl foam immobilized cyanobacteria. Photobiochem. Photobiophys. 12: 193–201.
American Public Health Association (1985) Standard methods for the examination of water and wastewater. APHA, Washington DC.
Bailliez C, Largeau C, Casadevall E, Yang LW, Berkaloff C (1988) Photosynthesis, growth and hydrocarbon production ofBotryococcus braunii immobilized by entrapment and adsorption in polyurethane foams. Appl. Microbiol. Biotechnol. 29: 141–147.
Bar-Or Y, Kessel M, Shilo M (1985) Modulation of cell-surface hydrophobicity in the benthic cyanobacteriumPhormidium J-1. Arch. Microbiol. 142: 21–27.
Brouers M, Shi DJ, Hall DO (1988) Immobilization methods for cyanobacteria in solid matrices. Methods Enzymol. 167: 629–636.
Castenholz RW (1970) Laboratory culture of thermophilic cyanophytes. Schweiz. Z. Hydrol. 32: 538–551.
Cheetham PSJ (1980) Developments in the immobilization of microbial cells and their applications. Topics Enzyme Ferment. Technol. 4: 189–238.
Chevalier P, de la Noüe J (1985a) Wastewater nutrient removal with microalgae immobilized in carrageenan. Enzyme Microb. Technol. 7: 621–624.
Chevalier P, de la Noüe J (1985b) Efficiency of immobilized hyperconcentrated algae for ammonium and orthophosphate removal from wastewaters. Biotechnol. Lett. 7: 395–400.
Comly HH (1945) Cyanosis in infants caused by nitrates in well water. J. Am. Med. Assoc. 129: 112–116.
de la Noüe J, Proulx D (1988) Tertiary treatment of urban wastewaters by chitosan-immobilizedPhormidium sp. In: Stadler T, Mollion J, Verdus MC, Karamanos Y, Morvan H, Christiaen D (eds), Algal Biotechnology. Elsevier Applied Science, Barking, 159–168.
de la Noüe J, Chevalier P, Proulx D (1990) Effluent treatment with immobilized microalgae and cyanobacteria. A critical assessment. In: Tyagi RD, Vembu K (eds), Wastewater treatment by immobilized cells. CRC Press, Boston, 143–153.
Dudley N (1990) Nitrates: the threat to food and water. Green Print, London, 118 pp.
Flores E, Romero JM, Guerrero MG, Losada M (1983) Regulatory interactions of photosynthetic nitrate utilization and carbon dioxide fixation in the cyanobacteriumAnacystis nidulans. Biochim. Biophys. Acta 725: 529–532.
Ford RC, Hefti A, Engel A (1990) Ordered arrays of the photosystem I reaction centre after reconstitution: projections and surface reliefs of the complex at 2 nm resolution. EMBO J. 9: 3067–3075.
Fowler HW (1988) Microbial adhesion to surfaces. In: Wimpenny JWT (ed.), CRC Handbook of Laboratory Model Systems for Microbial Ecosystems, Vol. 1. CRC Press, Inc., Boca Raton, 139–153.
Fraser P, Chilvers C (1981) Health aspects of nitrate in drinking water. In: van Lelyveld H, Zoeteman BCJ (eds), Water Supply and Health. Elsevier Scientific Publishing Company, Oxford, 103–116.
Garbisu C, Wen H, Hall DO, Serra JL (1990) Characteristics of photosystem activity of cyanobacteria immobilized in polymer foams. In: Baltscheffsky (ed.), Current Research in Photosynthesis, Vol. II, M. Kluwer Academic Publishers, Dordrecht, 699–702.
Guerrero MG, Lara C (1987) Assimilation of inorganic nitrogen. In: Fay P, van Baalen C (eds), The Cyanobacteria. Elsevier Science Publishers B.V. (Biomedical Division), Amsterdam, 163–186.
Hall DO, Affolter D, Brouers M, Shi DJ, Yang LW, Rao KK (1985) Photobiological production of fuels and chemicals by immobilized algae. Ann. Proceed. Phytochem. Soc., Europe 26: 161–185.
Hall DO, Rao KK (1989) Immobilized photosynthetic membranes and cells for the production of fuels and chemicals. Chimicaoggi, March: 40–47.
Lara C, Romero JM (1986) Distinctive light and CO2 fixation requirements of nitrate and ammonium utilization by the cyanobacteriumAnacystis nidulans. Plant Physiol. 81: 686–688.
Lara C, Romero JM, Guerrero MG (1987) Regulated nitrate transport in the cyanobacteriumAnacystis nidulans. J. Bacteriol. 169: 4376–4378.
Largeau C, Bailliez C, Yang LW, Frenz J, Casadevall E (1988) Immobilization of the microalgaBotryococcus braunii in calcium alginate gels and in polyurethane foams. Effect of immobilization on the alga metabolism-metabolite recovery by solvent extraction. In: Stadler T, Mollion J, Verdus Y, Karamanos H, Morvan H, Christiaen D (eds), Algal Biotechnology. Elsevier Applied Science, London, 245–253.
MacKinney G (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140: 315–322.
Malcome-Lawes DJ, Pasquini C (1988) A novel approach to non-segmented flow analysis. Part 3. Nitrate, nitrite and ammonium in waters. J. Autom. Chem. 10: 192–197.
Mohan RR, Li NN (1975) Nitrate and nitrite reduction by liquid membrane-encapsulated whole cells. Biotechnol. Bioeng. 17: 1137–1157.
Nilsson I., Ohlson S, Haggstrom L, Molin N, Mosbach K (1980) Denitrification of water using immobilizedPseudomonas denitrficans cells. Eur. J. Appl. Microb. Biotechnol. 10: 261–274.
Proulx D, de la Noüe J (1988) Removal of macronutrients from wastewater by immobilized algae. In: Moo-Young M (ed), Bioreactor immobilized enzymes and cells. Fundamentals and applications. Elsevier Applied Science, London, 301–310.
Rhodes MJC, Smith JI, Robins RJ (1987) Factors affecting the immobilization of plant cells on reticulated polyurethane foam particles. Appl. Microbiol. Biotechnol. 26: 28–35.
Robards AW, Crosby P (1979) A comprehensive freezing, fracturing and coating system for low temperature scanning electron microscopy. Scanning Electron Microsc. 2: 325–344.
Robins RJ, Hall DO, Shi DJ, Turner RJ, Rhodes MJC (1986) Mucilage acts to adhere cyanobacteria and cultured plant cells to biological and inert surfaces. FEMS Microbiol. Lett. 34: 155–160.
Serra JL, Arizmendi JM, Blanco F, Martínez-Bilbao M, Alaña A, Fresnedo O, Urkijo I, Llama MJ (1990) Nitrate assimilation in the non N2-fixing cyanobacteriumPhormidium laminosum. In: Ullrich WR, Rigano C, Fuggi A, Aparicio PJ (eds), Inorganic nitrogen metabolism. Springer Verlag, Berlin, pp. 196–202.
Serra JL, Llama MJ, Cadenas E (1978) Nitrate utilization by the diatomSkeletonema costatum. II. Regulation of nitrate uptake. Plant Physiol. 62: 991–994.
Serra JL, Ochoa de Alda JAG, Llama MJ (1988) Isolation and some properties of photosynthetic membrane vesicles enriched in photosystem I from the cyanobacteriumPhormidium laminosum by a non-detergent method. In: Grassi G, Hall DO (eds), Photocatalytic production of energy-rich compounds. Energy from Biomass 2. Commission of the European Communities, Elsevier Applied Science, London, 215–233.
Shi DJ (1987) Energy metabolism and structure of the immobilized cyanobacteriumAnabaena azollae. Ph.D. Thesis, King's College London, University of London.
Shi DJ, Brouers M, Hall DO, Robins RJ (1987) The effects of immobilization on the biochemical, physiological and morphological features ofAnabaena azollae. Planta 172: 298–308.
Solórzano L (1969) Determination of ammonia in natural water by the phenolhypochlorite method. Limnol. Oceanogr. 14: 799–801.
Sorokin C (1973) Dry weight, packed cell volume and optical density. In: Stein JR (ed.), Handbook of Phycological Methods. Cambridge University Press, Cambridge, 321–343.
Stewart AC, Bendall DS (1980) Photosynthetic electron transport in a cell-free preparation from the thermophilic blue-green algaPhormidium laminosum. Biochem. J. 188: 351–361.
Thepenier C, Gudin C (1985) Immobilization ofPorphyridium cruentum in polyurethane foams for the production of polysaccharide. Biomass 7: 225–240.
Tischner R, Lorenzen H (1979) Nitrate uptake and nitrate reduction in synchronousChlorella. Planta 146: 287–292.
Wildman W, Scarchilli D, Jagoe L (1988) Ion chromatography analysis of nitrate in drinking water: An update. J. Anal. Purif. 3: 50–53.
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Garbisu, C., Gil, J.M., Bazin, M.J. et al. Removal of nitrate from water by foam-immobilizedPhormidium laminosum in batch and continuous-flow bioreactors. J Appl Phycol 3, 221–234 (1991). https://doi.org/10.1007/BF00003580
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DOI: https://doi.org/10.1007/BF00003580