Abstract
The consumption, production and respiration of Amoeba proteus were measured in the laboratory for cells cultured over a range of Tetrahymena pyriformis concentrations (125–4 000 cells/0.5 ml) at 10, 15 and 20 °C. Differences were attributed to both temperature and prey availability. A series of generation energy budgets were constructed for amoebae grown under the above conditions. The biological efficiencies linking the parameters of the budget equation were calculated. Assimilation efficiencies ranged from 22–59% regardless of temperature. Net production efficiencies were high at 15 and 20 °C (65–82%) but low at 10 °C (11–49%). Gross production efficiencies were also higher at 15 and 20 °C (16–47%) than at 10 °C (4–29%). The ecological implications of this investigation are discussed.
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References
Chapman-Andresen, C., 1962. Studies on pinocytosis in amoebae. Compt. Rend. Trav. Lab. Carlsberg, 33: 73–264.
Curds, C. R. & Cockburn, A., 1968. Studies on the growth and feeding of Tetrahymena pyriformis in axenic and monoxenic culture. J. Gen. Microbiol., 54: 343–358.
Cutler, D. W. & Crump, L. M., 1924. The rate of reproduction in artificial culture of Colpidium colpoda. Biochem. J. 18: 905–912.
Finlay, B. J., 1977. The dependence of reproductive rate on cell size and temperature in freshwater ciliated protozoa. Oecologia, 30: 75–81.
Finlay, B. J., 1978. Community production and respiration by ciliated protozoa in the benthos of a small eutrophic loch. Freshwater Biology, 8: 327–341.
Goulder, R., 1971. Vertical distribution of some ciliated protozoa in two freshwater sediments. Oikos, 22: 199–203.
Grabacka, E., 1971. Ciliata in bottom sediment of fingerling ponds. Polskie Archiwum Hydrobiologie, 18: 225–233.
Griffin, J. L., 1960. Improved mass culture of amoebae. Exp. Cell Res., 21: 170–178.
Griffin, J. L., 1973. Culture: Maintenance, large Yields, and Problems of approaching Axenic Culture, pp. 83–98. In: Jeon, K. W. (Ed.), The Biology of Amoeba, Academic Press, New York and London.
Harding, J. P., 1937. Quantitative studies on the ciliate Glaucoma. 1. The regulation of the size and fission rate by the bacterial food supply. J. Exp. Biol., 14: 422–430.
Heal, O. W., 1967. Quantitative studies on soil amoebae. In: Progress in Soil Biology. O. Graff, and J. Satchell, eds. North Holland Publishing Co., Amsterdam, pp. 120–126.
Klekowski, R. Z., 1971. Cartesian diver microrespirometry for aquatic animals. Polskie Archiwum Hydrobiologii, 18: 93–114.
Klekowski, R. Z. & Fischer, Z., 1975. Review studies on ecological bioenergetics of aquatic animals. Polskie Archiwum Hydrobiologii, 22: 345–373.
Laybourn, J. E. M., 1975. Respiratory energy losses in Stentor coeruleus Ehrenberg (Ciliophora). Oecologia, 21: 273–278.
Laybourn, J. E. M., 1976a. Energy budgets for Stentor coeruleus Ehrenberg (Ciliophora). Oecologia, 22: 431–437.
Laybourn, J. E. M., 1976b. Energy consumption and growth in the suctorian Podophyra fixa (Protozoa: Suctoria). J. Zool. 180: 85–91.
Laybourn, J. E. M., 1976c. Respiratory energy losses in Podophyra fixa Muller in relation to temperature and nutritional status. J. Gen. Microbial. 96: 203–208.
Laybourn, J. E. M. & Stewart, J. M., 1975. Studies on consumption and growth in the ciliate Colpidium campylum Stokes. J. Anim. Ecol. 44: 165–174.
Legner, M., 1973. Experimental approach to the role of protozoa in aquatic ecosystems. Am. Zool. 13: 177–192.
Lindstrom-Lang, K., 1943. On the theory of the Cartesian diver microrespirometer. Compt. Rend. Trav. Lab. Carlsberg, 24: 333–398.
Mast, S. O., 1910. Reactions in Amoeba to light. J. Exp. Zool. 9: 265–277.
Mast, S. O., & Fennell, R. A., 1938. The relation between temperature, salts, hydrogen ion concentration and frequency of ingestion of food by Amoeba. Physiol. Zool. 11: 1–18.
Nardone, R. M., 1959. The controlled dixenic culture of Amoeba proteus with other observations of axenic cultures. J. Protozool., 6: Suppl. Abs. No. 28.
Pace, D. M. & Belda, W. H., 1944. The effect of food content and temperature on respiration in P. carolinensis Wilson. Biol. Bull., 86: 146–153.
Pace, D. M. & Kimura, K. K., 1944. The effect of temperature on the respiration of P. caudatum and P. aurelia. J. Cell. Comp. Physiol., 24: 173–183.
Pace, D. M. & Lyman, E. D., 1947. Oxygen consumption and carbon dioxide elimination in Tetrahymena gelii. Biol. Bull., 92: 210–216.
Phillipson, J., 1975. Introduction to ecological energetics. In: W. Grodzinski, R. Z. Klekowski and A. Duncan (Eds.) Methods for Ecological Bioenergetics, IBP Handbook No. 24. Blackwell Scientific Publ., Oxford and Edinburgh.
Prescott, D. M. & Carrier, R. D., 1964. Experimental procedures and culture methods for Euplotes eurystomus and Amoeba proteus. In: Methods in Cell Physiology, 1 D.M. Prescott, Ed. Academic Press, New York, pp. 85–95.
Proper, G. & Garver, J. C., 1966. Mass culture of the protozoa Colpoda steinii. Biotech. Bioeng. 8: 287–296.
Rogerson, A., 1979. Energy content of Amoeba proteus and Tetrahymena pyriformis (Protozoa) Can. J. Zool., 57: 2463–2465.
Rogerson, A., 1980. Generation times and reproductive rates of Amoeba proteus (Leidy) as influenced by temperature and food concentration. Can. J. Zool. 58: 543–548.
Salt, G. W., 1961. Feeding activity by A. proteus. Exp. Cell Res. 24: 618–620.
Sarojini, R. & Nagabhushanam, R., 1966. Exogenous factors affecting respiration in the ciliate, Spirostomum ambiguum. J. Anim. Morph. Physiol. 13: 95–102.
Sokal, R. R. & Rohlf, F. J., 1969. Biometry. W. H. Freeman and Co., San Francisco.
Taylor, L. R., 1961. Aggregation, variance and the mean. Nature, 189: 732–735.
Taylor, W. D., 1978. Growth responses of ciliate protozoa to the abundance of their bacterial prey. Microbial Ecol. 4: 207–214.
Welch, H. E., 1968. Relationships between assimilation efficiencies and growth efficiencies for aquatic consumers. Ecology, 49: 755–759.
Williamson, J. W., 1944. Nutrition and growth studies of Amoeba. Physiol. Zool., 17: 209–228.
Winberg, G. G., (Ed.), 1971. Symbols, units and conversion factors in studies in freshwater productivity. I.B.P. Section P.F. London.
Zeuthen, E., 1950. Cartesian diver respirometry. Bull. Mar. Biol. Lab. Woods Hole, 98: 139–143.
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Rogerson, A. The ecological energetics of Amoeba proteus (Protozoa). Hydrobiologia 85, 117–128 (1981). https://doi.org/10.1007/BF00006621
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DOI: https://doi.org/10.1007/BF00006621