Abstract
At last a fixation method that ensures tropical pseudocerotid polyclads are fixed flat, preserved for histological preparation and which also retains their colour pattern has been developed. FCA-PGPP (Formaldehyde Calcium Acetate-Propylene Glycol, Propylene Phenoxetol) fixative is frozen and worms are coaxed onto filter paper which is then laid on the frozen fixative. As a consequence, over 230 species have been documented from the southern Great Barrier Reef and eastern Papua New Guinea (Newman & Cannon, 1994; in press). It was determined that species diagnoses need to be based on colour pattern, general morphology of living animals and serial reconstructions of the male anatomy.
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Christensen Research Institute Contribution No. 82
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Newman, L.J., Cannon, L.R.G. The importance of the fixation of colour, pattern and form in tropical Pseudocerotidae (Platyhelminthes, Polycladida). Hydrobiologia 305, 141–143 (1995). https://doi.org/10.1007/BF00036376
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DOI: https://doi.org/10.1007/BF00036376