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Production of constitutive, thermostable, hyper active exo-pectinase from Bacillus GK-8

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Summary

A new alkalophilic Bacillus GK-8 overproduced three thermostable extracellular pectinases. The temperature optima was 60°C for all the three enzymes which retained full activity for 2 h. They had pH optima 5.4, 7.0 and 10.4 and were designated as PI, PII and PIII respectively. The half life at 80°C of PI, PII and PIII was 18 min., 12 min. and 12 min., respectively. The enzyme activity was stimulated by Mg2+, Ca2+, Zn2+, Co2+ and Mn2+ ions.

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Authors and Affiliations

  1. Department of Microbiology, Panjab University, 160014, Chandigarh, India

    Nirpjit Singh Dosanjh & G. S. Hoondal

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  1. Nirpjit Singh Dosanjh
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  2. G. S. Hoondal
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Singh Dosanjh, N., Hoondal, G.S. Production of constitutive, thermostable, hyper active exo-pectinase from Bacillus GK-8. Biotechnol Lett 18, 1435–1438 (1996). https://doi.org/10.1007/BF00129350

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  • DOI: https://doi.org/10.1007/BF00129350

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