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Modified lipase having high stability and various enzymic activities in benzene, and its re-use by recovering from benzene solution

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Summary

Lipoprotein lipase modified with polyethylene glycol dissolved in benzene, and catalyzed various reactions of ester synthesis, ester exchange and aminolysis. This modified enzyme had a high stability; 50% of the initial enzymic activity were retained after about 3 months-storage in benzene at room temperature. We can repeatedly re-use the enzyme by recovering from benzene solution; the enzyme precipitates upon addition of n-hexane(or petroleum ether).

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References

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Authors and Affiliations

  1. Laboratory of Biological Chemistry, Tokyo Institute of Technology Ookayama, Meguroku, 152, Tokyo, Japan

    T. Yoshimoto, K. Takahashi, H. Nishimura, A. Ajima, Y. Tamaura & Y. Inada

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  1. T. Yoshimoto
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  2. K. Takahashi
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  3. H. Nishimura
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  4. A. Ajima
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  5. Y. Tamaura
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  6. Y. Inada
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Yoshimoto, T., Takahashi, K., Nishimura, H. et al. Modified lipase having high stability and various enzymic activities in benzene, and its re-use by recovering from benzene solution. Biotechnol Lett 6, 337–340 (1984). https://doi.org/10.1007/BF00138001

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  • DOI: https://doi.org/10.1007/BF00138001

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