Summary
Up to 45% of bound cellulase (Avicelase) could be recovered from crystalline cellulose by pH adjustment with NaOH (0.008–0.01 M) to pH 10.0 (40°C). Enzyme was simultaneously desorbed and inactivated as the pH increased and both events occurred within 1 minute. Enzyme desorption and stability were also temperature dependent and desorption increased to approximately 65% by adding detergents.
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Otter, D.E., Munro, P.A., Scott, G.K. et al. Elution of Trichoderma reesei cellulase from cellulose by pH adjustment with sodium hydroxide. Biotechnol Lett 6, 369–374 (1984). https://doi.org/10.1007/BF00138007
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DOI: https://doi.org/10.1007/BF00138007