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Preparation of optically pure chiral amines by lipase-catalyzed enantioselective hydrolysis of N-acyl-amines

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Biotechnology Techniques

Summary

A new and effective method using lipase from Candida antarctica (native or Novozym SP-435) for the preparation of enantiopure primary R-amines is reported. Hydrolysis of rac-N-acetyl-amines resulted in high conversion > 40% (168 hrs) and > 48% (> 240 hrs) and enantiomeric excesses (> 99% ee) for both the product and the remaining substrate.

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Authors and Affiliations

  1. Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, D-70569, Stuttgart, Germany

    Hauke Smidt, Andreas Fischer & Rolf D. Schmid

  2. Institute of Organic Chemistry and Isotope Research, University of Stuttgart, Pfaffenwaldring 55, D-70569, Stuttgart, Germany

    Peter Fischer

Authors
  1. Hauke Smidt
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  2. Andreas Fischer
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  3. Peter Fischer
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  4. Rolf D. Schmid
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Smidt, H., Fischer, A., Fischer, P. et al. Preparation of optically pure chiral amines by lipase-catalyzed enantioselective hydrolysis of N-acyl-amines. Biotechnol Tech 10, 335–338 (1996). https://doi.org/10.1007/BF00173249

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  • DOI: https://doi.org/10.1007/BF00173249

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