Summary
A yeast potentiometric biosensor for glucose determination is described. After induction of glycolytic enzyme synthesis a cell suspension of the yeast Hansenula anomala is retained in calcium alginate gel on the surface of a glass electrode. This biosensor gives a Nernstian response in glucose concentration of 5·10−4–5·10−3 mol/l with a response time of 5 min and a life-time of at least 2 months. Mannose and fructose are the only significantly interfering substances. The biosensor was used for measurement of glucose concentration in urine with results comparable to those obtained by a photometric enzymatic method.
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References
Baudras A, Spyridakis A (1971) Etude de la l(+)lactate: cytochrome c oxydoréductase (cytochrome b2) de la levure Hansenula anomala. Biochimie 53:942–955
Grobler SR, Rechnitz GA (1980) Determination of d(+)glucose, d(+)mannose, d(+)galactose or d(−)fructose in a mixture of hexoses and pentoses by use of dental plaque coupled with a glass electrode. Talanta 27:283–285
Grobler SR, Wyk CW van (1980) Potentiometric determination of d(+)glucose, d(+)mannose or d(−)fructose in a mixture of hexoses and pentoses, by using Streptococcus mutans fermentation. Talanta 27:602–604
Karube I, Mitsuda S, Suzuki S (1979) Glucose sensor using immobilized whole cells of Pseudomonas fluorescens. Eur J Appl Microbiol Biotechnol 7:343–350
Kühn M, Hamann H, Böttcher N, Scheller F, Uffrecht E, Brunner J (1987) Analytical systems with potentiometric enzyme electrodes. Stud Biophys 119:171–174
Kulys J, Kadzinauskiénė K (1980) Yeast BOD sensor. Biotechnol Bioeng 22:221–226
Mascini M, Memoli A (1986) Comparison of microbial sensors based on amperometric and potentiometric electrodes. Anal Chim Acta 182:113–122
Matsumoto K, Seijo H, Watanabe T, Karube I, Satoh I, Suzuki S (1979) Immobilized whole cell-based flow-type sensor for cephalosporins. Anal Chim Acta 105:429–432
Nilsson H, Akerlund A-Ch, Mosbach K (1973) Determination of glucose, urea and penicillin using enzyme-pH-electrodes. Biochim Biophys Acta 320:529–534
Ogbonna JC, Amano Y, Nakamura K (1989) Elucidation of optimum conditions for immobilization of viable cells by using calcium alginate. J Ferment Bioeng 67:92–96
Racek J, Musil J (1987) Biosensor for lactate determination in biological fluids. I. Construction and properties of the biosensor. Clin Chim Acta 162:129–139
Vadgama P (1981) Enzyme electrodes as practical biosensors. J Med Eng Technol 5:293–298
Vais H, Oancea F, Fagliu AM, Delcea C, Margineanu DG (1985) Amperometric electrode for glucose with immobilized bacteria (Pseudomonas fluorescens). Rev Roum Biochim 22:55–63
Vincké BJ, Kaufmann J-M, Devleeschouwer MJ, Patriarche GJ (1984) Nouveau modèle d'électrode enzymatique pour la détermination du glucose. Analusis 12:141–147
Vincké BJ, Devleeschouwer MJ, Patriarche GJ (1985) Electrodes potentiometriques et amperometriques a levures permeabilises: determination du l-lactate. Anal Lett 18:593–609
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Racek, J. A yeast biosensor for glucose determination. Appl Microbiol Biotechnol 34, 473–477 (1991). https://doi.org/10.1007/BF00180573
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DOI: https://doi.org/10.1007/BF00180573