Summary
Growth and phenolic production by two heterotrophic suspension cultures (SW-1 and SW-2) of sandalwood cultivated in a 2.5 L bioreactor were investigated. Cultures of SW-1 cell suspensions resulted in a maximum phenolic content of 32.5 mg L−1 compared to 12.5 mg L−1 produced by SW-2 cell suspensions. Fresh weight doubling time (Td) was 5.8 days and the specific growth rate (μ) was 0.12 d−1 during exponential growth for both cell lines. The pH of the culture medium decreased from 5.5 to 3.5 during the exponential growth phase of SW-1 and SW-2 cell suspensions. The dissolved oxygen content also dropped steadily during culture and remained at 40% throughout exponential growth phase. These results should provide a basis for developing sandalwood cell cultures for bioproduction of useful compounds.
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Abbreviations
- 2,4-D: 2–4:
-
dichlorophenoxyacetic acid
- BA:
-
N6-benzyladenine
- Eh:
-
Medium oxidation-reduction potential
- KLa:
-
Oxygen transfer coefficient
- MS:
-
Murashige and Skoog (1962) basal medium
- SW-1 and SW-2:
-
Sandalwood suspension lines
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Communicated by G. C. Phillips
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Valluri, J.V., Treat, W.J. & Soltes, E.J. Bioreactor culture of heterotrophic sandalwood (Santalum album L.) cell suspensions utilizing a cell-lift impeller. Plant Cell Reports 10, 366–370 (1991). https://doi.org/10.1007/BF00193161
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DOI: https://doi.org/10.1007/BF00193161