Summary
The antimetastatic effect of Lactobacillus casei YIT9018 (LC 9018) against Lewis lung carcinoma (3LL) in C57BL/6 mice was determined. Intrapleural (i.pl.) administration of LC 9018 was effective in inhibiting pulmonary metastasis after s.c. inoculation of 3LL tumors into C57BL/6 mice. The combination of i.pl. and intralesional or i.v. injections of LC 9018 also markedly inhibited pulmonary metastasis in 3LL-bearing mice. The i.pl. administration of LC 9018 into mice induced an increase in the number of thoracic exudate cells (TEC) and the cell population in the TEC was mainly polymorphonuclear leukocytes in the early stage, while macrophages were dominant in the late stage. In addition, in vitro cytolytic activity against 3LL cells and natural killer cell activity of TEC were augmented by the i.pl. administration of LC 9018. Furthermore, i.pl. administration of LC 9018 into the mice rendered their lung macrophages tumoricidal for 3LL cells in vitro. These results show that TEC induced by i.pl. administration of LC 9018 played a key role in the inhibtion of metastasis in 3LL-bearing mice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Azuma I, Yamawaki M, Yasumoto K, Yamamura Y (1978) Antitumor activity of Nocardia cell wall skeleton preparation in transplantable tumors in syngeneic mice and patients with malignant pleurisy. Cancer Immunol Immunother 4:95
Gorelik E, Fogel M, Feldman M, Segal S (1979) Differences in resistance of metastatic tumor cells and cells from local tumor growth to cytotoxicity of natural killer cells. J Natl Cancer Inst 63:1397
Gorelik E, Segal S, Feldman M (1980) Control of lung metastasis progression in mice: Role of growth kinetics of 3LL Lewis lung carcinoma and host immune reactivity. J Natl Cancer Inst 65:1257
Hashimoto S, Nomoto K, Matsuzaki T, Yokokura T, Mutai M (1984) Oxygen radical production by peritoneal macrophages and Kupffer cells elicited with Lactobacillus casei. Infect Immun 44:61
Hashimoto S, Seyama Y, Yokokura T, Mutai M (1985) Cytotoxic factor production by Kupffer cells elicited with Lactobacillus casei and Corynebacterium parvum. Cancer Immunol Immunother 20:117
Herberman RB, Nunn ME, Holden HT, Lavrin DH (1975) Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells. Int J Cancer 16:230
Kato I, Kobayashi S, Yokokura T, Mutai M (1981) Antitumor activity of Lactobacillus casei in mice. Gann 72:517
Kato I, Yokokura T, Mutai M (1983) Macrophage activation of Lactobacillus casei in mice. Microbiol Immunol 27:611
Mantovani A, Sessa C, Peri G, Allavena P, Introna M, Polentarutti N, Mangioni C (1981) Intraperitoneal administration of Corynebacterium parvum in patients with ascitic ovarian tumors resistant to chemotherapy. Effects on cytotoxicity of tumor-associated macrophages and NK cells. Int J Cancer 27:437
Matsuzaki T, Yokokura T, Azuma I (1985) Antitumor activity of Lactobacillus casei on Lewis lung carcinoma and line-10 hepatoma in syngeneic mice and guinea pigs. Cancer Immunol Immunother 20:18
Matsuzaki T, Yokokura T, Azuma I (1987) Antimetastatic effect of Lactobacillus casei YIT9018 (LC 9018) on a highly metastatic variant of B16 melanoma in C57BL/6 mice. Cancer Immunol Immunother 24:99
McKneally MF, Maver C, Kausel H, Alley RD (1976) Regional immunotherapy with intrapleural BCG for lung cancer. J Thorac Cardiovasc Surg 72:333
Nomoto K, Miake S, Hashimoto S, Yokokura T, Mutai M, Yoshikai Y, Nomoto K (1985) Augmentation of host resistance to Listeria monocytogenes infection by Lactobacillus casei. J Clin Lab Immunol 17:91
Uchida A, Micksche M (1981) Natural killer cells in carcinomatous pleural effusions. Cancer Immunol Immunother 11:131
Uchida A, Micksche M (1983) Intrapleural administration of OK432 in cancer patients: Activation of NK cells and reduction of suppressor cells. Int J Cancer 31:1
Uchida A, Micksche M, Hoshino T (1984) Intrapleural administration of OK432 in cancer patients: Augmentation of autologous tumor killing activity of tumor-associated large granular lymphocytes. Cancer Immunol Immunother 18:5
Yamamura Y, Yasumoto K, Ogura T, Azuma I (1981) Nocardia rubra-cell wall skeleton in the therapy of animal and human cancer. In: Hersh EM et al. (eds) Augmentation agents in cancer therapy. Raven Press, New York, p 71
Yasumoto K, Manabe H, Ohta M, Nomoto K, Azuma I, Yamamura Y (1978) Immunotherapy of lung cancer and carcinomatous pleuritis. Gann Monogr Cancer Res 21:129
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matsuzaki, T., Yokokura, T. Inhibition of tumor metastasis of lewis lung carcinoma in C57BL/6 mice by intrapleural administration of Lactobacillus casei . Cancer Immunol Immunother 25, 100–104 (1987). https://doi.org/10.1007/BF00199948
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00199948