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Iron oxide impregnated filter paper (Pi test): a review of its development and methodological research

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Abstract

Iron oxide impregnated filter paper (FeO paper) has been used to study the availability of phosphorus (P) to plants and algae, P desorption kinetics and P dynamics in the field. Since its initial development a number of differences in the method of preparation of the paper and its application have been implemented. The purpose of this article is to (i) critically review studies on the various aspects of both preparation and use of the FeO paper and (ii) to suggest a standardized procedure.

Type of filter paper, FeO impregnation of the filter paper, neutralization of FeCl3, washing and drying of the impregnated paper, all have a significant effect on the amount of P sorbed. It is suggested that the method for neutralization of FeCl3 is checked on the release of acid when FeO paper is used, because the acid can release P compounds that would otherwise not desorb. Washing after neutralization is essential to remove loose FeO particles, which otherwise will cause incomplete P recovery. Modifications of the desorption procedure itself also affect the amount of P sorbed onto the paper. The ionic strength of the shaking solution, surface area of the FeO paper and the number and position of the paper in the shaking solution, and shaking strength and time all may introduce variations in the amount of P on the FeO paper. Differences in the dissolution of the Fe compounds on the filter paper and the subsequent determination of P in the solute may contribute to variations in P recovery. It is illustrated that the existing variation in the preparation and use of the FeO paper will cause differences in the amount of P extracted from soils, impeding the comparison of different studies.

Finally, we recommend a standardized procedure for the preparation and use of the FeO paper. Such a procedure will allow comparison between studies and may reduce or circumvent possible artefacts.

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References

  • AmerF, BouldinDR, BlackCA and DukeFR (1955) Characterization of soil phosphorus by anion exchange resin adsorption and P32-equilibration. Plant Soil 6: 391–408

    Google Scholar 

  • AndraskiBJ, MuellerDH and DanielTC (1985) Phosphorus losses in runoff as affected by tillage. Soil Sci Soc Am J 49: 1523–1527

    Google Scholar 

  • BarrowNJ (1978) The description of phospate adsorption curves. Soil Sci 29: 447–462

    Google Scholar 

  • BarrowNJ and ShawTC (1975) The slow reactions between soil and anions: 5. Effects of period of prior contact on the desorption of phosphate from soils. Soil Sci 119: 311–320.

    Google Scholar 

  • BarrowNJ and ShawTC (1979) Effects of ionic strength and nature of the cation on desorption of phosphate from soil. J Soil Sci 30: 53–65

    Google Scholar 

  • BramleyRGV and RoeSP (1993) Preparation of iron oxide- impregnated filter paper for use in the Pi test for soil phosphorus. Plant Soil 151: 143–146

    Google Scholar 

  • Buselli EM (1994) Evaluation of several high-affinity, high capacity, sinks for multielement release from soils, mine spoils, and sediments. PhD Thesis, Utah State University, Logan, Utah

  • ChaoTT and ZhouL (1983) Extraction techniques for selective dissolution of amorphous iron oxides from soils and sediments. Soil Sci Soc Am J 47: 225–232

    Google Scholar 

  • DorichRA, NelsonDW and SommersLE (1985) Estimating algal available phosphorus in suspended sediments by chemical extractions. J Environ Qual 14: 400–405

    Google Scholar 

  • Fokkink LGJ (1983) Desorption of phosphorus from soil: release by uptake (in Dutch). MSc Thesis, Wageningen Agricultural University

  • HsuPS and RichCI (1960) Aluminum fixation in a synthetic cation exchanger. Soil Sci Soc Am Proc 24: 21–25

    Google Scholar 

  • HuettlPJ, WendtRC and CoreyRB (1979) Prediction of algalavailable phosphorus in runoff suspensions. J Environ Qual 8: 130–132

    Google Scholar 

  • KuoS and JellumEJ (1994) The effect of soil phosphorus buffering capacity on phosphorus extraction by iron oxide-coated paper strips in some acid soils. Soil Sci 158: 124–131

    Google Scholar 

  • LahannRW (1976) Surface charge variation in aging ferric hydroxide. Clays Clay Miner 24: 320–326

    Google Scholar 

  • LewisDG (1992) Transformations induced in ferrihydrite by ovendrying. Z Pflanzenernähr Bodenkd 155: 461–466

    Google Scholar 

  • LinTH, HoSB and HoungKH (1991) The use of iron oxide-impregnated filter paper for the extraction of available phosphorus from Taiwan soils. Plant Soil 133: 219–226

    Google Scholar 

  • LookmanR, FreeseD, MerckexR, VlassakK and VanRiemsdijkWH (1995) Long-term kinetics of phosphate release from soil. Environ Sci Technol 29: 1569–1575

    Google Scholar 

  • MenonRG (1992) The Pi test for evaluating bioavailability of phosphorus. In: HoddinottKB and O'ShayTA (eds) Application of Agricultural Analysis in Environmental Studies, ASTM STP 1162, pp 58–67, Philadelphia, Amer Soc for Testing and Materials

    Google Scholar 

  • MenonRG, HammondLL and SissinghHA (1989a) Determination of plant-available phosphorus by the iron hydroxideimpregnated filter paper (Pi) soil test. Soil Sci Soc Am J 53: 110–115

    Google Scholar 

  • MenonRG, ChienSH and HammondLL (1989b) Comparison of Bray I and Pi tests for evaluating plant-available phosphorus from soils treated with different partially acidulated phosphate rocks. Plant Soil 114: 211–216

    Google Scholar 

  • Menon RG, Chien SH and Hammond LL (1989c) The Pi Soil Phosphorus Test: A New Approach to Testing for Soil Phosphorus. Reference Manual R-7 Muscle Shoals, Alabama International Fertilizer Development Center

  • MenonRG, ChienSH, HammondLL and HenaoJ (1989d) Modified techniques for preparing paper strips for the new Pi soil test for phosphorus. Fert Res 19: 85–91

    Google Scholar 

  • MenonRG, ChienSH and HammondLL (1990) Development and evaluation of the Pi soil test for plant-available phosphorus. Comm Soil Sci Pl Anal 21: 1131–1150

    Google Scholar 

  • MuellerDH, WendtRC and DanielTC (1984) Phosphorus losses as affected by tillage and manure application. Soil Sci Soc Am J 48: 901–905

    Google Scholar 

  • Myers RG, Pierzynski GM and Thien SJ (1994) Enhancements to the FeO-sink analysis for soil phosphorus. Agronomy Abstr. Wisconsin, USA, American Society Agronomy p 318

  • MyersRG, PierzynskiGM and ThienSJ (1995) Improving the iron oxide sink method for extracting soil phosphorus. Soil Sci Soc Am J 59: 853–857

    Google Scholar 

  • PerrottKW and WiseRG (1993) An evaluation of some aspects of the iron oxide-impregnated filter paper (Pi) test for available soil phosphorus with New Zealand soils. NZ J Agric Res 36: 157–162

    Google Scholar 

  • PierzynskiGM, MyersRG and ThienSJ (1994) Soil tests for phosphorus bioavailability. Trans 15th Int Congress Soil Sci, Acapulco, Mexico, 1994, vol 5b, pp 119–120

    Google Scholar 

  • RobargeWP and CoreyRB (1979) Adsorption of phosphate by hydroxy-aluminum species on a cation exchange resin. Soil Sci Soc Am J 43: 481–487

    Google Scholar 

  • RobinsonJS and SharpleyAN (1994) Organic phosphorus effects on sink characteristics of iron-oxide-impregnated filter paper. Soil Sci Soc Am J 58: 758–761

    Google Scholar 

  • RobinsonJS, SharpleyAN and SmithSJ (1994) Development of a method to determine bioavailable phosphorus loss in agricultural runoff. Agric Ecosyst Environ 47: 287–297

    Article  Google Scholar 

  • SchwertmannU (1988) Occurrence and formation of iron oxides in various pedoenvironments. In: StuckiJW, GoodmanBA & SchwertmannU (eds), Iron in Soils and Clay Minerals, pp 267–308, Dordrecht, Holland, Reidel Publ Comp

    Google Scholar 

  • SchwertmannU and CornellRM (1991) Iron oxides in the laboratory. Weinheim, VCH

    Google Scholar 

  • SharpleyAN (1993) An innovative approach to estimate bioavailable phosphorus in agricultural runoff using iron oxide impregnated paper. J Environ Qual 22: 597–601

    Google Scholar 

  • SharpleyAN, IndiatiR, CiavattaC, RossiN and SequiP (1994) Interlaboratory comparison of iron oxide-impregnated paper to estimate bioavailable phosphorus. J Environ Qual 23: 14–18

    Google Scholar 

  • SharpleyAN, RobinsonJS and SmithSJ (1995) Bioavailable phosphorus dynamics in agricultural soils and effects on water quality. Geoderma 67: 1–15

    Article  Google Scholar 

  • SissinghHA (1971) Analytical technique of the Pw method, used for the assessment of the phosphate status of arable soils in the Netherlands. Plant Soil 34: 483–486

    Google Scholar 

  • SissinghHA (1983) Estimation of plant-available phosphates in tropical soils. A new analytical technique. Haren, Netherlands, Inst Soil Fertility Research

    Google Scholar 

  • TrainaSJ, SpositoG, HesterbergD and KafkakiU (1986) Effects of pH and organic acids on orthophosphate solubility in an acidic, montmorillonitic soil. Soil Sci Soc Am J 50: 45–52

    Google Scholar 

  • Van derZeeSEATM, FokkinkLGJ and VanRiemsdijkWH (1987) A new technique for assessment of reversibly adsorbed phosphate. Soil Sci Soc Am J 51: 599–604

    Google Scholar 

  • Yli-HallaM (1989) Reversibly adsorbed P in mineral soils of Finland. Comm Soil Sci Pl Anal 20: 695–709

    Google Scholar 

  • WendtRC and CoreyRB (1980) Phosphorus variations in surface runoff from agricultural lands as a function of land use. J Environ Qual 9: 130–136

    Google Scholar 

  • WhelanBR, WittwerK, RoeSP, BarrowNJ and BramleyRGV (1994) An air brush and water to clean the iron oxide strips in the Pi soil test for phosphorus. Trans 15th Int Congress Soil Sci, Acapulco, Mexico, 1994, vol 5b pp 166–167

    Google Scholar 

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Authors and Affiliations

  1. DLO Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 129, 9750 AC, Haren, The Netherlands

    W. J. Chardon

  2. Research and Development Division, International Fertilizer Development Center (IFDC), P.O. Box 2040, 35662, Muscle Shoals, Alabama, USA

    R. G. Menon & S. H. Chien

Authors
  1. W. J. Chardon
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  2. R. G. Menon
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  3. S. H. Chien
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Chardon, W.J., Menon, R.G. & Chien, S.H. Iron oxide impregnated filter paper (Pi test): a review of its development and methodological research. Nutr Cycl Agroecosyst 46, 41–51 (1996). https://doi.org/10.1007/BF00210223

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  • DOI: https://doi.org/10.1007/BF00210223

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