Abstract
Hairy root cultures of Centranthus ruber DC. were established by infection of sterile plantlets with Agrobacterium rhizogenes, strain R1601. The transformed roots were grown in 12 different, hormone-free liquid media, and valtrate, isovaltrate, 7-desisovaleroyl-7-acetylvaltrate, 7-homovaltrate, didrovaltrate and isovaleroxyhydroxydidrovaltrate were quantified by high performance liquid chromatography. The highest overall valepotriate content (3.0% dry wt) was observed in half-strength Gamborg B5 medium supplemented with 3% sucrose. This concentration is very similar to that found in the roots of parent plants grown in the field. The use of N,N-dimethylmorpholinium iodide, a plant bioregulator, was very detrimental to the hairy root growth and to the valepotriate production. The hairy roots cultured in half strength Gamborg B5 liquid medium supplemented with 3% sucrose for 45 days produced over 31 mg/g dry wt valepotriates.
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Abbreviations
- MS:
-
Murashige and Skoog medium (Murashige and Skoog 1962)
- B5:
-
Gamborg B5 medium (Gamborg 1970)
- WP:
-
McCown's woody plant medium (Lloyd and McCown 1980)
- H:
-
Heller's medium (Heller 1953)
- 1/4 B5-7:
-
quarter strength B5 + 7% sucrose
- DMI:
-
N,N-dimethylmorpholmium iodide
- VAL:
-
valtrate
- IVAL:
-
isovaltrate
- DIA-VAL:
-
7-desisovaleroyl-7-acetylvaltrate
- HVAL:
-
7-homovaltrate
- DI:
-
didrovaltrate
- IVHD:
-
isovaleroxyhydroxydidrovaltrate (Fig. 1)
- NMR:
-
nuclear magnetic resonance spectroscopy
- HPLC:
-
high performance liquid chromatography
- Ac:
-
acetyl
- IV:
-
isovaleryl
- IV-IV:
-
α-isovaleryloxy-isovaleryl
- MV:
-
β-methyl-valeryl
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Gränicher, F., Christen, P. & Kapétanidis, I. Production of valepotriates by hairy root cultures of Centranthus ruber DC. Plant Cell Reports 14, 294–298 (1995). https://doi.org/10.1007/BF00232031
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DOI: https://doi.org/10.1007/BF00232031