Abstract
Both Alcaligenes eutrophus JMP 134 and its plasmid-free derivative Alcaligenes eutrophus JMP 222 utilize 2,6-dinitrophenol as sole source of carbon, energy, and nitrogen. In the presence of ammonia resting cells of these strains release two mol of nitrite per mol of 2,6-dinitrophenol. Alcaligenes eutrophus JMP 222-α1D, a mutant of strain JMP 222 obtained by transposon (Tn5) mutagenesis, is able to use 2,6-dinitrophenol as nitrogen source but not as source of carbon and energy. Resting cells of this mutant liberate only one mol of nitrite per mol of 2,6-dinitrophenol. A single metabolite was detected by high-pressure liquid chromatography and identified as 2-hydroxy-5-nitropenta-2,4-dienoic acid from the mass spectrum, the 1H-, and 13C-NMR spectra. Strain JMP 222-α1S, a spontaneous mutant of strain JMP 222-α1D, accumulates 4-nitropyrogallol which was identified as the initial metabolite of 2,6-dinitrophenol degradation.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- 2,6-DNP:
-
2,6-dinitrophenol
- HNMA:
-
2-hydroxy-5-nitromuconic acid
- HNPA:
-
2-hydroxy-5-nitropenta-2,4-dienoic acid
- NB:
-
nutrient broth
- NMR:
-
nuclear magnetic resonance
- NPG:
-
4-nitropyrogallol
- O.D.:
-
optical density
- tR :
-
retention time
- UV/Vis:
-
ultraviolet/visible
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Ecker, S., Widmann, T., Lenke, H. et al. Catabolism of 2,6-dinitrophenol by Alcaligenes eutrophus JMP 134 and JMP 222. Arch. Microbiol. 158, 149–154 (1992). https://doi.org/10.1007/BF00245219
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DOI: https://doi.org/10.1007/BF00245219