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Production of l-glutamate by immobilized protoplasts

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Summary

Protoplasts of Brevibacterium flavum cultured in a medium containing 50 μg·l-1 of biotin were prepared with lysozyme and immobilized in matrices of agar-acetylcellulose filters. The immobilized protoplasts were applied to l-glutamate production from glucose and urea in a batch system. The productivity of l-glutamate by the immobilized protoplasts was 2.5 times higher than that by immobilized whole cells under optimal conditions. Maximal productivity initially reached 1.5 mg·ml-1. The immobilized protoplasts of B. flavum could be used six times for l-glutamate production with retention of about 70% of the initial productivity.

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Author notes

  1. Hideaki Matsuoka

    Present address: Department of Industrial Chemistry, Tokyo University of Agriculture and Technology, 184, Koganei, Tokyo, Japan

  2. Shuichi Suzuki

    Present address: Faculty of engineering, The Saitama Institute of Technology, Okabe-cho, Ohsato-gun, 369-02, Saitama, Japan

Authors and Affiliations

  1. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, 227, Yokohama, Japan

    Isao Karube, Masahiro Kawarai, Hideaki Matsuoka & Shuichi Suzuki

Authors
  1. Isao Karube
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  2. Masahiro Kawarai
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  3. Hideaki Matsuoka
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  4. Shuichi Suzuki
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Karube, I., Kawarai, M., Matsuoka, H. et al. Production of l-glutamate by immobilized protoplasts. Appl Microbiol Biotechnol 21, 270–272 (1985). https://doi.org/10.1007/BF00252702

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  • DOI: https://doi.org/10.1007/BF00252702

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