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Immunological determination of hydroxylated 6α-fluor-16α-methyl deoxycorticosterone derivatives in fungal steroid transformation

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Summary

Antisera and monoclonal antibodies were raised against the 9α and 11β hydroxylated derivatives of 6α-fluor-16α-methyl deoxycorticosterone via 3-0-carboxy-methyloxim-bovine serum albumin immunogenic conjugates. The properties of the antibodies and several immunoassay systems were evaluated including an enzyme immunoassay with nitrocellulose as the solid phase and an insoluble, colored reaction product allowing visual evaluation of assays. The performance of the techniques was evaluated for the industrial important fungus, Curvularia lunata.

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Abbreviations

AP:

alkaline phosphatase

BCIP:

5-bromo-4-chloro-3-indolyl phosphate

BSA:

bovine serum albumin

DMF:

dimethyl formamide

EDC:

1-ethyl-3-(3-dimethylaminopropyl)carbodiimide

ELISA:

enzyme linked immunosorbent assay

FMDOC:

6α-fluor-16α-methyl deoxycorticosterone

MAB:

monoclonal antibodies

NC:

nitrocellulose

RIA:

radioimmunoassay

TBS:

TRIS buffered saline

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Pflanzenphysiologie, Universität Osnabrück, Postfach 4469, D-4500, Osnabrück, Federal Republic of Germany

    E. W. Weiler & J. Eberle

  2. Mikrobiologische Entwicklung, Schering AG, Postfach 650311, D-1000, Berlin-Charlottenburg

    M. Droste, H. J. Halfman & A. Weber

Authors
  1. E. W. Weiler
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  2. M. Droste
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  3. J. Eberle
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  4. H. J. Halfman
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  5. A. Weber
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Dedicated to Professor Dr. H. J. Rehm on the occasion of his 60th birthday

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Weiler, E.W., Droste, M., Eberle, J. et al. Immunological determination of hydroxylated 6α-fluor-16α-methyl deoxycorticosterone derivatives in fungal steroid transformation. Appl Microbiol Biotechnol 27, 252–259 (1987). https://doi.org/10.1007/BF00252927

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  • DOI: https://doi.org/10.1007/BF00252927

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