Summary
The kinetics of production of transducing particles for different bacterial markers were followed by premature lysis of Salmonella typhimurium cells infected with P22 phages. They were compared for cells infected with wild type phage or with HT-mutants which show increased transduction frequencies. Measuring the sedimentation velocity of bacterial DNA of cells infected with wild type or HT-phages, it was shown that: a) there is no cutting of DNA at random; b) original fragments necessary for packaging host DNA into transducing particles cannot be smaller than 10 phage-genome lengths; c) cutting of transducing fragments leads immediately to the right length; d) there is no loss of precipitable DNA due to phage infection.
Similar content being viewed by others
References
Botstein, D.: Synthesis and maturation of phage P22 DNA. I. Idendification of intermediates. J. molec. Biol. 34, 621–641 (1968)
Botstein, D., Waddell, C. H., King, J.: Mechanism of head assembly and DNA encapsulation in Salmonella phage P22. I. Genes, protiens, structures and DNA maturation. J. molec. Biol. 80, 669–695 (1973)
Chelala, C. A., Margolin, P.: Effects of deletions on cotransduction linkage in Salmonella typhimurium: Evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments. Molec. gen. Genet. 131, 97–112 (1974)
Harriman, P.: Appearance of transducing activity in P1-infected Escherichia coli. Virology 45, 324–325 (1971)
Raj, A. S., Raj, A. Y., Schmieger, H.: Phage genes involved in the formation of generalized transducing particles in Salmonella phage P22. Molec. gen. Genet. 135, 175–184 (1974)
Rhoades, M., MacHattie, L. A., Thomas, C. A., Jr.: The P22 bacteriophage DNA molecule. I. The mature form. J. molec. Biol. 37, 21–40 (1968)
Schmieger, H.: Die molekulare Struktur transduzierender Partikel beim Salmonella-Phagen P22. I. Dichtegradienten-Untersuchungen an intakten Phagen. Molec. gen. Genet. 102, 336–347 (1968)
Schmieger, H.: The molecular structure of the transducing particles of Salmonella phage P22. II. Density gradient analysis of DNA. Molec. gen. Genet. 109, 323–337 (1970)
Schmieger, H.: The fate of the bacterial chromosome in P22-infected cells of Salmonella typhimurium. Molec. gen. Genet. 110, 238–244 (1971)
Schmieger, H.: Phage P22-mutants with increased or decreased transduction abilities. Molec. gen. Genet. 119, 75–88 (1972)
Strätling, W., Krause, E., Knippers, R.: Fast sedimenting deoxyribonucleic acid in bacteriophage T7-infected cells. Virology 51, 109–119 (1973)
Streisinger, G., Emrich, J., Stahl, M. M.: Chromosome structure in phage T4. III. Terminal redundancy and length determination. Proc. nat. Acad. Sc. (Wash.) 57, 292–295 (1967)
Tye, B., Huberman, J. A., Botstein, D.: Non-random circular permutation of phage P22 DNA. J. molec. Biol. 85, 501–532 (1974)
Zinder, N.: Bacterial transduction. J. cell. comp. Physiol. 45, 23–49 (1955)
Author information
Authors and Affiliations
Additional information
Communicated by W. Maas
Rights and permissions
About this article
Cite this article
Schmieger, H., Buch, U. Appearance of transducing particles and the fate of host DNA after infection of Salmonella typhimurium with P22-mutants with increased transducing ability (HT-mutants). Molec. gen. Genet. 140, 111–122 (1975). https://doi.org/10.1007/BF00329779
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00329779