Abstract
Pseudomonas cepacia MBA4 able to utilize monobromoacetic acid as a sole source of carbon and energy was isolated from soil by enrichment culture. In batch culture the ability to utilize the substrate was conferred by a single halidohydrolase-type dehalogenase which demonstrated a high activity towards the enrichment substrate. The purified enzyme, designated as dehalogenase IVa by activity-stain polyacrylamide gel electrophoresis, had a relative molecular weight of 45,000 and was comprised of two electrophoretically identical subunits with relative molecular weights of 23,000. Dehalogenase IVa demonstrated isomer specificity, being active towards the L-isomer of 2-monochloropropionic acid only. The significance of activity-stain polyacrylamide gel electrophoresis in characterizing dehalogenases and their ubiquitous distribution among bacterial genera are discussed.
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Abbreviations
- MCA:
-
Monochloroacetic acid
- DCA:
-
dichloroacetic acid
- MBA:
-
monobromoacetic acid
- 2MPCA:
-
2-monochloropropionic acid
- 2MBPA:
-
2-monobromopropionic acid
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Tsang, J.S.H., Sallis, P.J., Bull, A.T. et al. A monobromoacetate dehalogenase from Pseudomonas cepacia MBA4. Arch. Microbiol. 150, 441–446 (1988). https://doi.org/10.1007/BF00422284
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DOI: https://doi.org/10.1007/BF00422284