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Immunochemical detection of ochratoxin A in black Aspergillus strains

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Abstract

One hundred and fifty-seven strains belonging to Aspergillus section Nigri were tested for ochratoxin A production using three different methods: a relatively new immunochemical method based on an enzyme-linked immunosorbent assay (ELISA), thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The monoclonal antibody-based ELISA technique was successfully used to screen for low levels of ochratoxin A in the black Aspergilli without concentrating the culture filtrates. The results were confirmed by TLC and HPLC analysis and chemical derivatization. These latter methods required concentrated filtrates. Ochratoxin A was detected in the culture filtrates of five of the 12 A. carbonarius strains, none of the 45 A.japonicus strains and three of the 100 isolates in the A. niger aggregate (A. foetidus, A. awamori and A. niger).

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Abbreviations

ELISA:

enzyme-linked immunosorbent assay

HPLC:

high-performance liquid chromatography

OA:

ochratoxin A

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Animal Health and Food Control Station, P.O. Box 446, H-6701, Szeged, Hungary

    József Téren

  2. Department of Microbiology, Attila József University, P.O. Box 533, H-6701, Szeged, Hungary

    János Varga, Zsuzsanna Hamari, Edit Rinyu & Ferenc Kevei

Authors
  1. József Téren
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  2. János Varga
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  3. Zsuzsanna Hamari
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  4. Edit Rinyu
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  5. Ferenc Kevei
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Correspondence to János Varga.

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Téren, J., Varga, J., Hamari, Z. et al. Immunochemical detection of ochratoxin A in black Aspergillus strains. Mycopathologia 134, 171–176 (1996). https://doi.org/10.1007/BF00436726

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  • DOI: https://doi.org/10.1007/BF00436726

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