Summary
The autometallographic procedure represents a new technique that can substitute for the normal methods of physical development (PD). The physical developer (a solution of reducing substance, silver salt and protection colloid) is replaced by a photographic emulsion and chemical developer. Accumulations of gold, silver, metal sulphides and metal selenides can be amplified by the present technique.
Tissue sections placed on glass slides are covered by a silver bromide containing emulsion, dried and exposed to a chemical developer. After development the emulsion is either removed or cleared and the sections are counterstained and embedded.
The autometallographic procedure can also be applied to ultrathin sections.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brunk U, Brun A, Skjöld G (1968) Histochemical demonstration of heavy metals with sulfide-silver method. A methodological study. Acta Histochem 31:345–357
Danscher G (1981a) Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electron microscopy. Histochemistry 71:1–16
Danscher G (1981b) Localization of gold in biological tissue. A photochemical method for light and electron microscopy. Histochemistry 71:81–88
Danscher G (1981c) Light and electron microscopic localization of silver in biological tissue. Histochemistry 71:177–186
Danscher G (1982) Exogenous selenium in the brain. A histochemical technique for light and electron microscopical localization of catalytic selenium bonds. Histochemistry 76:281–293
Danscher G (1984) A silver method for counterstaining plastic embedded tissue. Stain Technol (in press)
Danscher G, Møller-Madsen B (1984) Silver amplification of mercury sulphides and selenides. A histochemical method for light and electron microscopic localization of mercury in tissue. J Histochem Cytochem (in press)
Danscher G, Nørgaard JOR (1983) Light microscopic visualization of colloidal gold on resin-embedded tissue. J Histochem Cytochem 31:1394–1398
Danscher G, Nørgaard JOR (1984) Ultrastructural autometallography. A method for silver amplification of catalytic metals. J Histochem Cytochem (in press)
Gallyas F (1971) A principle for silver staining of tissue elements by physical development. Acta Morphol Acad Sci Hung 19:57–71
Gallyas F (1979) Light insensitive physical developers. Stain Technol 54:173–176
Gallyas F (1980) Silver staining of photoplasmic astrocytes by physical development. Acta Morphol Acad Sci Hung 29:169–176
Gallyas F, Lénárd L, Lázár GY (1978) Improvement of cobalt-transport in axons by complexing agents. Neurosci Lett 9:213–216
James TH (1939) The reduction of silver ions by hydroquinone. J Am Chem Soc 61:648–658
James TH (1977) The theory of the photographic process. Macmillan: New York, Chapt 13
Liesegang RE (1911) Die Kolloidchemie der histologischen Silber-Färbungen. Kolloid Beihefte 3:1–46
Merker B (1983) Silver staining of cell bodies by means of physical development. J Neurosci Methods 9:235–242
Pearson AA, O'Neil SL (1946) A silver gelatin method for staining nerve fibers. Anat Rec 95:297–301
Roberts WJ (1935) A new procedure for the detection of gold in animal tissue. Proc R Acad (Amsterdam) 38:540–544
Timm F (1958) Zur Histochemie der Schwermetalle, das Sulfid-Silber-Verfahren. Dtsch Z Ges Gerichtl Med 46:706–711
Umeda M, Saito K, Saito M (1969) Cytotoxic effect of inorganic, phenyl and alkyl mercuric compounds on hela cells. Jpn J Exp Med 39:17–58
Weil A, Davenport H (1930) Eine Methode zur Silberimprägnierung von Gliomen. Z Neurol Psychiatr 126:796–802
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Danscher, G. Autometallography. Histochemistry 81, 331–335 (1984). https://doi.org/10.1007/BF00514327
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00514327