Abstract
The subcellular location of aspartate aminotransferase isozymes (EC 2.6.1.1) in the genusCapsella(Brassicaceae) was studied. The diploid speciesC. grandiflora andC. rubella have three AAT isozymes, including one located in the plastids. Each locus is duplicated in the tetraploidCapsella bursa-pastoris. Variation at the plastid-coding locus exceeded that at the other loci.C. bursa-pastoris had some unique alleles not detected in the diploid species. Segregation in open-pollinated families revealed thatCapsella grandiflora was outcrossing, whereasC. rubella was highly inbred, with most populations homozygous or uniform at all three loci. Inheritance in the tetraploid colonizerC. bursa-pastoris is disomic. This species was also predominantly selfing with outcrossing rates between 2% and 10%.
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Financial support by the German Research Foundation DFG is gratefully acknowledged.
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Hurka, H., Freundner, S., Brown, A.H.D. et al. Aspartate aminotransferase isozymes in the genusCapsella (Brassicaceae): Subcellular location, gene duplication, and polymorphism. Biochem Genet 27, 77–90 (1989). https://doi.org/10.1007/BF00563019
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DOI: https://doi.org/10.1007/BF00563019