Abstract
Strips of intact and chemically skinned (Triton X-100) taenia coli were mounted for isometric and quick-release experiments at 23°C. Active force increased in repeated high-K+ induced contractures in the intact muscle. Stable maximal force was 313±24 mN/mm2 (n=6). The skinned preparations activated by Ca2+, at 2 mM Mg2+, 3.2 mM MgATP and ionic strength 0.085 M, gave half maximal force atpCa=5.62±0.4 and a maximal force (63±8 mN/mm2) atpCa=4.5 (20–25 of the control K+-responses prior to skinning but about 60% of the first K+-response). Force-velocity relations were obtained from intact muscles and from the same muscles chemically skinned and activated at optimal Ca2+. Maximal shortening velocity (V max) was unaltered in the skinned preparation compared to the intact muscle (0.138±0.011 vs 0.140±0.006 L/s) indicating similar kinetics of actomyosin interaction. In the intact muscle a decrease inV max was found when the Ca2+ concentration was reduced. Calmodulin (1μM) increased Ca2+ sensitivity (by about 0.6 log units) of the skinned preparation but at optimal Ca2+ caused no alteration in isometric force orV max
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References
Åberg AKG, Axelsson J (1965) Some mechanical aspects of an intestinal smooth muscle. Acta Physiol Scand 64: 15–27
Adelstein RS, Eisenberg E (1980) Regulation and kinetics of the actinmyosin-ATP interaction. Ann Rev Biochem 49: 921–956
Barany M (1967) ATPase activity of myosin correlated with speed of muscle shortening. J Gen Physiol 50: 197–218
Barron JT, Barany M, Barany K (1979) Phosphorylation of the 20,000-dalton light chain of myosin of intact arterial smooth muscle in rest and in contraction. J Biol Chem 254: 4954–4956
Barron JT, Barany M, Barany K, Storti RV (1980) Reversible phosphorylation and dephosphorylation of the 20,000-dalton light chain of myosin during the contraction-relaxation-contraction cycle of arterial smooth muscle. J Biol Chem 255: 6238–6244
Burnstock G, Campbell G, Rand MJ (1966) The inhibitory innervation of the taenia of the guinea-pig caecum. J Physiol (Lond)182: 504–526
Cassidy PS, Kerrick WGL, Hoar PE, Malencik DA (1981) Exogenous calmodulin increases Ca2+ sensitivity of isometric tension activation and myosin phosphorylation in skinned smooth muscle. Pflügers Arch 392: 115–120
Dabrowska R, Sherry JMF, Aromatorio DK, Hartshorne DJ (1978) Modulator protein as a component of the myosin light chain kinase from chicken gizzard. Biochemistry USA 17: 253–258
Dillon PF (1980) Calcium dependence of force and velocity in vascular smooth muscle. Fed Proc 39: 1733
Dillon PF, Aksoy MO, Driska SP, Murphy RA (1981) Myosin phosphorylation and the cross-bridge cycle in arterial smooth muscle. Science 211: 495–497
Driska SP, Aksoy MO, Murphy RA (1981) Myosin light chain phosphorylation associated with contraction in arterial smooth muscle. Am J Physiol 240: C222-C233
Endo M, Kitazawa T, Yagi S, Iino M, Kakuta Y (1977) Some properties of chemically skinned smooth muscle fibers. In: Casteels R, Godfraind T, Rüegg JC (eds) Excitation-contraction coupling in smooth muscle. North-Holland Publishing Co., Amsterdam, pp 199–209
Fabiato A, Fabiato F (1979) Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 75: 463–505
Fletcher R, Powell JD (1963) A rapidly convergent descent method for minimization. Computer J 6: 163–168
Gabella G (1976) The force generated by a visceral smooth muscle. J Physiol (Lond) 263: 199–213
Gabella G (1981) Structure of smooth muscles. In: Bülbring E, Brading AF, Jones AW, Tomita T (eds) Smooth muscle. Edward Arnold, London, pp 1–46
Godt RE, Maughan DW (1977) Swelling of skinned muscle fibers of the frog. Experimental observations. Biophys J 19: 103–116
Godt RE, Maughan DW (1981) Influence of osmotic compression on calcium activation and tension in skinned muscle fibers of the rabbit. Pflügers Arch 391: 334–337
Gordon AR (1978) Contraction of detergent-treated smooth muscle. Proc Natl Acad Sci USA 75: 3527–3530
Hellstrand P, Johansson B (1975) The force-velocity relation in phasic contractions of venous smooth muscle. Acta Physiol Scand 93: 157–166
Hellstrand P, Johansson B (1979) Analysis of the length response to a force step in smooth muscle from rabbit urinary bladder. Acta Physiol Scand 106: 221–238
Hellstrand P, Johansson B, Ringberg A (1972) Influence of extracellular calcium on isometric force and velocity of shortening in depolarized venous smooth muscle. Acta Physiol Scand 84: 528–537
Hoar PE, Kerrick WGL, Cassidy PS (1979) Chicken gizzard: Relation between calcium-activated phosphorylation and contraction. Science 204: 503–506
Iino M (1981) Tension responses of chemically skinned fibre bundles of the guinea-pig taenia caeci under varied ionic environments. J Physiol (Lond) 320: 449–467
Johansson B, Hellstrand P, Uvelius B (1978) Responses of smooth muscle to quick load change studied at high time resolution. Blood Vessels 15: 65–82
Jones AW, Somlyo AP, Somlyo AV (1973) Potassium accumulation in smooth muscle and associated ultrastructural changes. J Physiol (Lond) 232: 247–273
Lowy J, Mulvany MJ (1973) Mechanical properties of guinea pig taenia coli muscles. Acta Physiol Scand 88: 123–136
Marston SB, Trevett RM, Walters M (1980) Calcium ion-regulated thin filaments from vascular smooth muscle. Biochem J 185: 355–365
Mashima H, Handa M (1969) The force-velocity relation and the dynamic constants of the guinea-pig taenia coli. J Physiol Soc Jpn 31: 565–566
Mikawa T, Toyo-oka T, Nonomura Y, Ebashi S (1977) Essential factor of gizzard troponin fraction. J Biochem 81: 273–275
Parker RB, Waud DR (1971) Pharmacological estimation of drugreceptor dissociation constants. Statistical evaluation. I. Agonists. J Pharmacol Exp Ther 177: 1–12
Peiper U, Ehl M, Johnson U, Laven R (1976) Force velocity relations in vascular smooth muscle: The influence of pH, pCa and noradrenaline. Pflügers Arch 364: 135–141
Peterson JW (1980) Vanadate ion inhibits actomyosin interaction in chemically skinned vascular smooth muscle. Biochem Biophys Res Commun 95: 1846–1853
Peterson JW (1982) Simple model of smooth muscle myosin phosphorylation and dephosphorylation as rate-limiting mechanism. Biophys J 37: 453–459
Saida K, Nonomura Y (1978) Characteristics of Ca2+-and Mg2+-induced tension development in chemically skinned smooth muscle fibers. J Gen Physiol 72: 1–14
Schneider M, Sparrow M, Rüegg JC (1981) Inorganic phosphate promotes relaxation of chemically skinned smooth muscle of guinea pig taenia coli. Experientia 37: 980–983
Sjölin L, Hellstrand P, Clementz B (1978) An apparatus for mechanical experiments on isolated smooth muscle. Acta Physiol Scand 102: 32A-33A
Sparrow MP, Mrwa U, Hofmann F, Rüegg JC (1981) Calmodulin is essential for smooth muscle contraction. FEBS Lett 125: 141–145
Spedding M (1981) Comparison of “Ca++-antagonists” and trifluoperazine in skinned smooth muscle fibres. Br J Pharmacol 75: 25P
Uvelius B (1979) Shortening velocity, active force and homogeneity of contraction during electrically evoked twitches in smooth muscle from rabbit urinary bladder. Acta Physiol Scand 106: 481–486
Uvelius B, Hellstrand P (1980) Effects of phasic and tonic activation on contraction dynamics in smooth muscle. Acta Physiol Scand 109: 399–406
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Apreliminary report of some of the results presented here was given at the Scandinavian Physiology Society Meeting in Århus, November 1981. Arner A, Hellstrand P (1982) Acta Physiol Scand (Abstract) 114: 38 A.
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Arner, A. Mechanical characteristics of chemically skinned guinea-pig taenia coli. Pflugers Arch. 395, 277–284 (1982). https://doi.org/10.1007/BF00580790
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DOI: https://doi.org/10.1007/BF00580790