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Atomic force microscopy of plant chromosomes

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Abstract

Atomic force microscopy has been used to image plant chromosomes from standard preparations without staining or coating. This has enabled the collection of high-resolution three-dimensional data on surface structure. The technique has been further applied to the imaging of C-banded chromosomes revealing structural changes resulting from the banding treatment. The bands were observed as localized areas of high relief.

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References

  • Bustamante C, Keller D, Yang G (1993) Scanning force microscopy of nucleic acids and nucleoprotein assemblies.Curr Opin Struct Biol 3: 363–372.

    Google Scholar 

  • De Grooth BG, Putman CJA (1992) High-resolution imaging of chromosome-related structures by atomic force microscopy.Microscopy 168: 239–247.

    Google Scholar 

  • Fritzsche W, Schaper A, Jovin TM (1994) Probing chromatin with the scanning force microscopeChromosoma 103: 231–236.

    Google Scholar 

  • Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum).Genome 34: 830–839.

    Google Scholar 

  • Harrison CJ, Britch M, Allen TD, Harris R (1981) Scanning electron microscopy of the G-banded human karyotype.Exp Cell Res 134: 141–153.

    Google Scholar 

  • Harrison CJ, Allen TD, Harris R (1983) Scanning electron microscopy of variations in human metaphase chromosome structure revealed by Giemsa banding.Cytogenet Cell Genet 35: 21–27.

    Google Scholar 

  • Harrison CJ, Jack EM, Allen TD, Harris R (1985) Light and scanning electron microscopy of the same human metaphase chromosomes.Cell Sci 77: 143–153.

    Google Scholar 

  • Jack EM, Harrison CJ, Allen TA, Harris R (1985) The structural basis for C-banding: A scanning electron microscopy study.Chromosoma 91: 363–368.

    Google Scholar 

  • McMaster TJ, Hickish T, Min T, Cunningham D, Miles MJ (1994) Application of scanning force microscope to chromosome analysis.Cancer Genet Cytogenetics 76: 93–95.

    Google Scholar 

  • Rasch P, Wiedemann U, Wienberg J, Heckl WM (1993) Analysis of banded human chromosomes andin situ hybridization patterns by scanning force microscopy.Proc Natl Acad Sci USA 90: 2509–2511.

    Google Scholar 

  • Sanchez-Sweatman OH, de Harven EP Dubé ID (1993) Human chromosome evaluation of processing techniques for scanning electron microscopy.Scanning Microscopy 7: 97–106.

    Google Scholar 

  • Schaper A, Pietrasanta LI, Jovin TM (1993) Scanning force microscopy of circular and linear DNA spread on mica with a quaternary ammonium salt.Nucleic Acids Res 21: 6004–6009.

    Google Scholar 

  • Sumner AT, Ross A (1989) Factors affecting preparation of chromosomes for scanning electron microscopy using osmium impregnation.Scanning Microscopy 3: 87–99.

    Google Scholar 

  • Thomson NH, Miles MJ, Ring SG, Shewry PR, Tatham AS (1994) Real-time imaging of enzymic degradation of starch granules by atomic force microscopy.J Vacuum Sci Technol 12: 1565–1568.

    Google Scholar 

  • Wanner G, Formanek H, Martin R, Herrman RG (1991) High resolution scanning electron microscopy of plant chromosomesChromosoma 100: 103–109.

    Google Scholar 

  • Winfield M, Davey M, Karp A (1993) A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats.Heredity 70: 187–194.

    Google Scholar 

  • Yang J, Tamm LK, Somlyo AP, Shao Z (1993) Promises and problems of biological atomic force microscopy.J Microscopy 171: 183–198

    Google Scholar 

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Correspondence to T. J. McMaster.

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Winfield, M., McMaster, T.J., Karp, A. et al. Atomic force microscopy of plant chromosomes. Chromosome Res 3, 128–131 (1995). https://doi.org/10.1007/BF00710674

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  • DOI: https://doi.org/10.1007/BF00710674

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