Abstract
The use of15N as a tracer in soil/plant research is examined. The limitations of the so-called Ndff approach are discussed to show the need to consider not just the fate of the added label but also the path that was followed and the rate of the transformation. The development of15N isotope dilution techniques to determine gross rates of nitrogen transformation in soil is reviewed with some indications as to the further development of the approach.
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References
Barraclough D (1991a) The use of mean pool abundances to interpret nitrogen-15 tracer experiments. I. Theory. Plant and Soil 131: 89–96
Barraclough D (1991b) The use of mean pool abundances to interpret nitrogen-15 tracer experiments. II. Application. Plant and Soil 131: 97–105
Barraclough D, Geens EL, Davies GP & Maggs JM (1985) Fate of fertilizer nitrogen. III. The use of single and double labelled15N ammonium nitrate to study nitrogen uptake by ryegrass. J Soil Sci 36: 593–603
Barrie A (1991) New methodologies in stable isotope analysis. In: Stable Isotopes in Plant Nutrition, Soil Fertility and Environmental Studies. International Atomic Energy Agency, Vienna Austria,
Bjarnason S (1988) Calculation of gross nitrogen immobilization and mineralization in soil. J Soil Sci 39: 393–406
Bremner J (1965) Inorganic forms of nitrogen. In: Black CA (ed) Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties. Agronomy 9, pp 1179–1237. Am Soc Agron, Madison, Wisconsin, USA
Brookes PD, Stark JM McInteer BB & Preston T (1989) Diffusion method to prepare soil extracts for automated nitrogen-15 analysis. Soil Sci Soc Am J 53: 1707–1711
Chalk PM, Victoria RL, Muraoka T & Piccolo MC (1990) Effect of a nitrification inhibitor on immobilization and mineralization of soil and fertilizer nitrogen. Soil Biol Biochem 22: 533–538
Davidson EA, Hart SC Shanks CA & Firestone MK (1991) Measuring gross nitrogen mineralization, immobilization, and nitrification by15N isotopic pool dilution in intact soil cores. J Soil Sci 42: 335–349
Kirkham D & Bartholomew WV (1954) Equations for following nutrient transformations in soil, utilizing tracer data. Soil Sci Soc Am Proc 18:33–34
Kirkham D & Bartholomew WV (1955) Equations for followin nutrient transformations in soil utilizing tracer data. II. Soil Sci Soc Am ProcI9: 189–192
Myrold DD & Tiedje JM (1986) Simultaneous estimation of several nitrogen cycle rates using15N. Theory and application. Soil Biol Biochem 18: 559–568
Nishio T, Kanamori T & Fujimoto T (1985) Nitrogen tranformations in an aerobic soil as determined by a15NH4 dilution technique. Soil Biol Biochem 17: 149–154
Pilbeam CJ, Mahapatra BS & Wood M (1993) Soil matric potential effects on gross rates of nitrogen mineralization in an Orthic Ferralsol from Kenya. Soil Biol Biochem 25: 1409–1414
Powlson DS & Barraclough D (1992) Mineralization and assimilitation in soil-plant systems. In: Knowles R & Blackburn TH (eds) Nitrogen Isotope Techniques. pp 209–242. Academic Press, London UK