Abstract
A strain ofThiobacillus ferrooxidans MAL-4-1 was adapted to grow at higher concentrations of copper by repeated subculturing in the presence of increasing levels of added cupric ions in 9K medium. The strains adapted to copper were found to be more efficient in bioleaching of copper from concentrates. When copper tolerant strains were back cultured repeatedly in 9K medium without cupric ions, the initially developed metal tolerance was observed to be lost. This indicates that the copper tolerance developed is stress-dependent and not a permanent trait of the adapted strain.
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References
Brahmaprakash GP, Devasia P, Jagadish KS, Natarajan KA & Ramananda Rao G (1988) Development ofThiobacillus ferrooxidans ATCC 19859 strain tolerant to copper and zinc. Bull. Mat. Sci. 10: 461–465
Brierley CL (1978) Bacterial leaching. Crit. Rev. Microbiol. 6: 207–262
Natarajan KA (1985) Microbe-mineral interactions of relevance in the hydrometallurgy of complex sulphides. In: Progress in Metallurgical Research-Fundamental and Applied Aspects. Tata McGraw Hill, New Delhi, pp. 105–112
Natarajan KA & Iwasaki I (1983) Role of galvanic interactions in the bioleaching of Duluth gabbro Copper-nickel sulfides. Separation Science & Technology 18: 1095–1111
Natarajan KA, Iwasaki I & Reid KJ (1983) Some aspects of microbe-mineral interactions of interest to Duluth gabbro Copper-nickel sulfides. In: Rossi G & Torma AE (Eds.) Recent Progress in Biohydrometallurgy. Associazione Mineraria Sarda, Italy, pp. 169–183
Silverman MP & Lundgren DG (1959) Studies on the chemoautotrophic iron bacteriumFerrobacillus ferrooxidans I. An improved medium and a harvesting procedure for securing high yields. J. Bacteriol. 77: 642–647
Tuovinen OH, Niemela SI & Gyllenberg HG (1971) Tolerance ofThiobacillus ferrooxidans to some metals. Antonie van Leeuwenhoek 37: 489–496
Yates JR, Cunningham RR & Holmes DS (1988) IST2: A new insertion sequence inThiobacillus ferrooxidans. Proc. Natl., Acad Sci. USA 85: 7284–7287
Yates JR & Holmes DS (1987) Two families of repeated DNA sequences inThiobacillus ferrooxidans. J. Bacteriol. 169: 1861–1870
Vishniac WW (1974)Thiobacillus, pp. 456–461. In: Buchanan RE & Gibbons ME (Eds.) Bergey's manual of determinative bacteriology, 8th ed. The Williams & Wilkins Co., Baltimore.
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Natarajan, K.A., Sudeesha, K. & Ramananda Rao, G. Stability of copper tolerance inThiobacillus ferrooxidans . Antonie van Leeuwenhoek 66, 303–306 (1994). https://doi.org/10.1007/BF00882764
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DOI: https://doi.org/10.1007/BF00882764