Abstract
The stoichiometric oxidation of uranous-to uranyl-uranium byThiobacllus ferrooxidans is demonstrated. Fixation of14CO2 and the effect of inhibitors demonstrate that energy is conserved during the oxidation and used for energy-dependent “reverse electron flow” and carbon dioxide fixation.
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Abbreviations
- HOQNO:
-
2-heptyl-4-hydroxyquinoline-N-oxide
- 8-HQ:
-
8-hydroxyquinoline
- TTFA:
-
thenoyltrifluoroacetone
References
Aleem MIH, Lees H, Nicholas DJD (1963) Adenosine triphosphate-dependent reduction of nicotinamide adenine dinucleotide by ferrocytochromec in chemoautotrophic bacteria. Nature (London) 200:759–761
Beck JV (1969)Thiobacillus ferrooxidans and its relation to the solubilization of ores of copper and iron. In: Perlman D (ed) Fermentation advances. Academic Press, New York, pp 747–771
Cobley JG, Haddock BA (1975) The respiratory chain ofThiobacillus ferrooxidans: the reduction of cytochromes by Fe2+ and the preliminary characterization of rusticyanin a novel ‘blue’ copper protein. FEBS Lett 60:29–33
Cox JC, Boxer DH (1978) The purification and some properties of rusticyanin, a blue copper protein in iron(II) oxidation fromThiobacillus ferro-oxidans. Biochem J 174:497–502
DiSpirito AA, Tuovinen OH (1981) Oxygen uptake coupled with uranous sulfate oxidation byThiobacillus ferrooxidans andT. acidophilus. Geomicrobiol J 2:275–291
DiSpirito AA, Tuovinen OH (1982) Kinetics of uranous ion and ferrous iron oxidation byThiobacillus ferrooxidans. Arch Microbiol 133: 33–37
Gale NL, Beck JV (1967) Evidence for the Calvin cycle and hexose monophosphate pathway inThiobacillus ferrooxidans. J Bacteriol 94:1052–1059
Ingledew WJ, Cox JC, Halling PJ (1977) A proposed mechanism for energy conservation during Fe2+ oxidation byThiobacillus ferro-oxidans: chemiosmotic coupling to net H+ influx. FEMS Microbiol Lett 2:193–197
Ivarson KC (1980) Enhancement of uranous-ion oxidation byThiobacillus ferrooxidans. Curr Microbiol 3:253–254
King MT, Drews G (1975) The respiratory electron transport system of heterotrophically-grownRhodopseudomonas palustris. Arch Microbiol 102:219–231
Lewis AJ, Miller JDA (1977) Stannous and cuprous ion oxidation byThiobacillus ferrooxidans. Can J Microbiol 23:319–324
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275
Martin PAW, Dugan PR, Tuovinen OH (1981) Plasmid DNA in acidophilic, chemolithotrophic thiobacilli. Can J Microbiol 27:850–853
Nielsen AM, Beck JV (1972) Chalcocite oxidation and coupled carbon dioxide fixation byThiobacillus ferrooxidans. Science 175:1124–1126
Soljanto P, Tuovinen OH (1980) A microcalorimetric study of U(IV)-oxidation byThiobacillus ferrooxidans and ferric-iron. In: Trudinger PA, Walter MR, Ralph BJ (eds) Biogeochemistry of ancient and modern environments. Springer, New York, pp 469–475
Tuovinen OH, Kelly DP (1973) Studies on the growth ofThiobacillus ferrooxidans. I. Use of membrane filters and ferrous iron agar to determine viable numbers, and comparison with14CO2-fixation and iron oxidation as measures of growth. Arch Mikrobiol 88:285–298
Tuovinen OH, Kelly DP (1974a) Studies on the growth ofThiobacillus ferrooxidans. III. Influence of uranium, other metal ions and 2:4-dinitrophenol on ferrous iron oxidation and carbon dioxide fixation by cell suspensions. Arch Microbiol 95:165–180
Tuovinen OH, Kelly DP (1974b) Use of micro-organisms for the recovery of metals. Int Metall Rev 19:21–31
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DiSpirito, A.A., Tuovinen, O.H. Uranous ion oxidation and carbon dioxide fixation byThiobacillus ferrooxidans . Arch. Microbiol. 133, 28–32 (1982). https://doi.org/10.1007/BF00943765
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DOI: https://doi.org/10.1007/BF00943765