Abstract
Kinetic constants for the oxidation of uranous and ferrous ions byThiobacillus ferrooxidans were estimated. The kinetics indicate a direct biological mechanism for uranium oxidation. The complex interrelations of ferric, uranyl and uranous ion inhibition are considered.
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Barbič FF (1977) Effects of different compounds of metals and of their mixtures on the growth and survival ofThiobacillus ferrooxidans. Z All Mikrobiol 17:277–281
DiSpirito AA, Silver M, Voss, L, Tuovinen OH (1982) Flagella and pili of iron-oxidizing thiobacilli isolated from a uranium mine in northern Ontario, Canada. Appl Environ Microbiol 43:1196–1200
DiSpirito AA, Tuovinen OH (1981) Oxygen uptake coupled with uranous sulfate oxidation byThiobacillus ferrooxidans andT. acidophilus. Geomicrobiol J 2:275–291
DiSpirito AA, Tuovinen OH (1982) Uranous ion oxidation and carbon dioxide fixation byThiobacillus ferrooxidans. Arch Microbiol 133: 28–32
Ebner HG, Schwartz W (1974) Geomikrobiologische Untersuchungen. XII. Verhalten von Mikroorganismen auf uranhaltigen Gesteinen. Z Allg Mikrobiol 14:93–102
Fisher JR (1966) Bacterial leaching of Elliot Lake uranium ore. Can Min Metall Bull 59:588–592
Harrison VF, Gow WA, Hughson MR (1966) I. Factors influencing the application of bacterial leaching to a Canadian uranium ore. J Metals 18:1189–1194
Ivarson KC (1980) Enhancement of uranous-ion oxidation byThiobacillus ferrooxidans. Curr Microbiol 3:253–254
Kelly DP, Jones CA (1978) Factors affecting metabolism and ferrous iron oxidation in suspensions and batch cultures ofThiobacillus ferrooxidans: relevance to ferric iron leach solution regeneration. In: Murr LE, Torma AE, Brierley JA (eds) Metallurgical application of bacterial leaching and releated microbiological phenomena. Academic Press, New York, pp 19–44
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275
Miller RP, Napier E, Wells RA, Audsley A, Daborn GR (1963) Natural leaching of uranium ores. Trans Instn Min Metall 72:217–254
Roy P, Mishra AK (1981) Iron oxidation not coupled to growth inThiobacillus ferrooxidans in the presence of toxic metals. J Appl Bacteriol 51:387–392
Soljanto P, Tuovinen OH (1980) A microcalorimetric study of U(IV)-oxidation byThiobacillus ferrooxidans and ferric-iron. In: Trudinger PA, Walter MR, Ralph BJ (eds) Biogeochemistry of ancient and modern environments. Springer, New York, pp 469–475
Tomizuka N, Yagisawa M (1978) Optimum conditions for leaching of uranium and oxidation of lead sulfide withThiobacillus ferrooxidans and recovery of metals from bacterial leaching solution with sulfate-reducing bacteria. In: Murr LE, Torma AE, Brierley JA (eds) Metallurgical applications of bacterial leaching and related microbiological phenomena. Academic Press, New York, pp 321–344
Tomizuka N, Yagisawa M, Someya J, Takahara Y (1976) Continuous leaching of uranium byThiobacillus ferrooxidans. Agr Biol Chem 40:1019–1025
Tuovinen OH, Kelly DP (1973) Studies on the growth ofThiobacillus ferrooxidans. I. Use of membrane filters and ferrous iron agar to determine viable numbers, and comparison with14CO2-fixation and iron oxidation as measures of growth. Arch Mikrobiol 88:285–298
Tuovinen OH, Kelly DP (1974a) Studies on the growth ofThiobacillus ferrooxidans. II. Toxicity of uranium to growing cultures and tolerance conferred by mutation, other metal cations and EDTA. Arch Microbiol 95:153–164
Tuovinen OH, Kelly DP (1974b) Use of micro-organisms for the recovery of metals. Int Metall Rev 19:21–31
Tuovinen OH, Silver M, Martin PAW, Dugan PR (1981) The Agnew Lake uranium mine leach liquors: chemical examinations, bacterial enumeration, and composition of plasmid DNA of iron-oxidizing thiobacilli. In: Proceedings of the International Conference on Use of Microorganisms in Hydrometallurgy, Hungarian Academy of Sciences, Pécs, pp 59–69
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DiSpirito, A.A., Tuovinen, O.H. Kinetics of uranous ion and ferrous iron oxidation byThiobacillus ferrooxidans . Arch. Microbiol. 133, 33–37 (1982). https://doi.org/10.1007/BF00943766
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DOI: https://doi.org/10.1007/BF00943766