Skip to main content
SpringerLink
Log in

Subtilisin-catalyzed transesterification in supercritical carbon dioxide

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Subtilisin Carlsberg was found to catalyze transesterification between N-acetyl-L-phenylalanine chloroethyl ester and ethanol in supercritical carbon dioxide. The effects of different temperatures and carbon dioxide/ethanol ratios on the reaction rate were investigated. A comparative study showed that enzymatic transesterification is faster in supercritical carbon dioxide than in anhydrous organic solvents.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Carrea, G., Riva, S., Secundo, F. and Danieli, B. (1989), J. Chem. Soc. Perkin Trans. I, 1057–1061.

    Google Scholar 

  • Dordick, J.S. (1989), Enzyme Microb. Thecnol. 11, 194–211.

    Google Scholar 

  • Hammond, D.A., Karel, M, Klibanov, A.M. and Krukonis, V.J. (1985), Appl. Biochem. Biotechnol. 11, 393–400.

    Google Scholar 

  • Khmelnitsky, Y.L., Levashov, A.V., Klyachko, N.L. and Martinek, K. (1988), Enzyme Microb. Technol. 10, 710–724.

    Google Scholar 

  • Klibanov, A.M. (1989), Trends Biochem. Sci. 14, 141–144.

    Google Scholar 

  • Nakamura, K., Min Chi, Y. and Yano, T. in “Proceedings of the International Symposium on Supercritical Fluids”. pp 925–931, 1988, Nice.

  • Randolph, T.W., Blanch, H.W., Prausnitz, J.M. and Wilke, C.R. (1985), Biotechnol. Lett. 7, 325–328.

    Google Scholar 

  • Randolph, T.W., Clark, D.S., Blanch, H.W. and Prausnitz, J.N. (1988), Science 387–390.

  • Riva, S., Chopineau, J., Kieboom, A.P.G. and Klibanov, A.M. (1988), J. Am. Chem. Soc. 110, 584–589.

    Google Scholar 

  • Schneider, G.M., Stahl, E. and Wilke, G., eds., Extraction with Supercritical Gases, Verlag Chemie, Weinheim, 1980.

    Google Scholar 

  • Smith, R.M., ed., Supercritical Fluid Chromatography, The Royal Society of Chemistry, London, 1988.

    Google Scholar 

  • Van Eijs, A.M.M., De Jong, J.P.L., Doddema, H.J. and Lindeboom, D.R. in “Proceedings of the International Symposium on Supercritical Fluids” pp 933–942, 1988, Nice.

  • Zaks, A. and Kilbanov, A.M. (1988), J. Biol. Chem. 263, 3194–3201.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Chimica degli Ormoni, C.N.R., Via Mario Bianco 9, 20131, Milano, Italy

    Pasta P., Carrea G. & Riva S.

  2. Jasco s.r.l., Via Confalonieri 25, Cremella (Como), Italy

    Mazzola G.

Authors
  1. Pasta P.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mazzola G.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carrea G.
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Riva S.
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pasta, P., Mazzola, G., Carrea, G. et al. Subtilisin-catalyzed transesterification in supercritical carbon dioxide. Biotechnol Lett 11, 643–648 (1989). https://doi.org/10.1007/BF01025275

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01025275

Keywords

Search

Navigation