Summary
Acrylamide was enzymatically produced from acrylonitrile (AN) in a two-stage packed bed reactor with immobilizedBrevibacterium sp. CH2 cells. Continuous production became possible because of higher tolerance of CH2 nitrile hydratase with respect to 6% AN than either the CH1 enzyme or theCorynebacterium enzyme of Nitto Chemical Industry which rapidly deactivated in a solution of 3% AN. The maximum volumetric productivity of 93.2 g/(L.h) was obtained when the reactor was fed with 6% AN at 4 °C, which compares that of 8.3 g/(L.h) with CH1 cells.
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Lee, C.Y., Chang, H.N. Continuous production of acrylamide using immobilizedBrevibacterium sp. CH2 in a two-stage packed bed reactor. Biotechnol Lett 12, 23–28 (1990). https://doi.org/10.1007/BF01028487
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DOI: https://doi.org/10.1007/BF01028487