Summary
Lipase fromCandida cylindracea was coupled with polyethylene glycol(PEG). In contrast to the previously used lipase fromPseudomonas fluorescens, the modified lipase catalyzed the ester synthesis in benzene at 25°C from short-chain alcohols and α- or β-substituted carboxylic acid. Using the modified lipase, following esters were synthesized; pentyl α-methylpentanate, methyl benzoate, and methyl retinoate.
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Kodera, Y., Takahashi, K., Nishimura, H. et al. Ester synthesis from α-substituted carboxylic acid catalyzed by polyethylene glycol-modified lipase fromCandida cylindracea in benzene. Biotechnol Lett 8, 881–884 (1986). https://doi.org/10.1007/BF01078652
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DOI: https://doi.org/10.1007/BF01078652