Summary
Cyclosporin A was produced in semi-synthetic and synthetic media byTolypocladium inflatum in suspension culture. L-valine increased the specific production of cyclosporin A by 62% in semi-synthetic media and by 4 times in synthetic media, compared to an unsupplemented control culture. D-valine had no stimulatory effect on the production. L-leucine was also a strong enhancer of drug production in synthetic media. Experiments with different times of addition of L-valine indicated that the amino acid may need to be present in exponential growth phase for optimal production. 4g/l was the optimum concentration of exogenous L-valine. In contrast, exogenous L-methionine and sarcosine tended to diminish the production of cyclosporin A.
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Lee, J., Agathos, S.N. Effect of amino acids on the production of cyclosporin a byTolypocladium inflatum . Biotechnol Lett 11, 77–82 (1989). https://doi.org/10.1007/BF01192178
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DOI: https://doi.org/10.1007/BF01192178