Abstract
Styrene/2-hydroxyethyl methacrylate polymer microspheres consisting of various polymer compositions were produced by emulsifier-free seeded emulsion polymerization technique. Using these microspheres, which should have hydrophilic/hydrophobic heterogeneous surface, the effects of surface hydrophilicity on the main, fundamental requirements for an immunomicrosphere — high colloidal stability, sensitive immunologic agglutinability and insensitive non-specific agglutinability — were studied in detail. There was a region of the surface hydrophilicity that satisfied the three requirements simultaneously.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Matsumoto T, Okubo M, Imai T (1975) Kobunshi Ronbunshu 32:229
Matsumoto T, Okubo M, Onoe S (1976) Kobunshi Ronbunshu 33:565
Singer JM, Plotz CM (1956) Am J Med 21:888
Robbins JL, Hill GA, Carle BN, Carlquist JH, Marcus C (1962) Proc Soc Exp Biol Med 109:321
Grinbnau TCJ, Leuvering JHW, Hell van H (1986) J Chromatogr Biomedical Appl 376:175
So R (1978) Nippon Houigakushi 30:152
Matsuzawa S (1982) Nippon Houigakushi 36:826
Okubo M, Uno M, Yamamoto Y, Kamei S, Matsumoto T (1987) Kobunshi Ronbunshu 44:123
Kamei S, Okubo M, Matsumoto T (1985) Nippon Kagaku Kaishi 1985:1307
Kamei S, Okubo M, Matsuda T, Matsumoto T (1986) Coll & Polym Sci 264:743
Kamei S, Okubo M, Matsumoto T (1986) J Polym Sci Polym Chem Ed 24:3109
Okubo M, Uno M, Yamamoto Y, Kamei S, Matsumoto T (1987) Kobunshi Ronbunshu 44:825
Matsuhashi S, Seinai H, Usui M (eds) (1978) Guide of Experiments of Immunology, Academic press, p 100
Hoy KL (1979) J Coating Technol 51:27
Okubo M, Nakamura Y, Asakura M, Tange T, Yamashita S, Matsumoto T (1982) Kobunshi Ronbunshu 39:345
Okubo M, Yamada A, Matsumoto T (1981) J Polym Sci Polym Chem Ed 19:1
Maron SH, Elder ME, Moore C (1954) J Coll Sci 9:104
Vijayendran BR (1980) In: Fitch M (ed) Polymer Colloid II, Plenum Press, p 209
MacRitchie F (1972) J Coll Interf Sci 38:484
Klein F, Valkenburg HA, Zwet TL van, Lafeber GM (1966) Immunology 10:87
Author information
Authors and Affiliations
Additional information
Part CII of the series “Studies on Suspension and Emulsion”.
Rights and permissions
About this article
Cite this article
Okubo, M., Yamamoto, Y., Uno, M. et al. Immunoactivity of polymer microspheres with their hydrophilic/hydrophobic heterogeneous surface sensitized with an antibody. Colloid & Polymer Sci 265, 1061–1066 (1987). https://doi.org/10.1007/BF01417463
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01417463