Abstract
The mechanism of adherence ofStaphylococcus epidermidisto commercially available catheters was studied in vitro in a quantitative assay employing3H-labelled bacteria. It was found that adherence to Teflon catheters was significantly related to the degree of hydrophobicity of the strains. When hydrophobic groups were removed fromStaphylococcus epidermidisby pepsin treatment, adhesion was almost completely abolished. Preincubation of catheters in human serum also caused a 80–90 % reduction of adherence. Preincubation ofStaphylococcus epidermidisin serum similarly decreased adhesion. This effect of serum was mainly due to albumin, while IgG and fibronectin were less effective. Culture ofStaphylococcus epidermidisin subinhibitory concentrations (0.5 MIC) of cephalothin, clindamycin and vancomycin resulted in a 30–80 % reduction in adhesion.
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Christensen, G. D., Bisno, A. L., Parisi, J. T., McLaughlin, B., Hester, M. G., Luther, R. W.: Nosocomial septicemia due to multiply antibiotic-resistantStaphylococcus epidermidis. Annals of Internal Medicine 1982, 96: 1–10.
Battisti, O., Mitchison, R., Davies, P.A.: Changing blood culture isolates in a referral neonatal intensive care unit. Archives of Disease in Childhood 1981, 5: 775–778.
Munson, D. P., Thompson, T. R., Johnson, D. E., Rhame, F. S., Van Drunen, N., Kennedy, D.: Coagulasenegative staphylococcal septicemia: experience in a newborn intensive care unit. Journal of Pediatrics 1982, 101: 602–605.
Fleer, A., Senders, R. C., Visser, M. R., Bijlmer, R. P., Gerards, L. J., Kraaijeveld, C. A., Verhoef, J.: Septicemia due to coagulase-negative staphylococci in a neonatal intensive care unit: clinical and bacteriological features and contaminated parenteral fluids as a source of sepsis. Pediatric Infectious Disease 1983, 2: 426–431.
Archer, G. L.:Staphylococcus epidermidis and other coagulase-negative staphylococci. In: Mandell, G. L., Douglas, R. G., Bennett, J. E. (ed.): Principles and practice of infectious diseases. Wiley Medical Publications, New York, 1984, p. 1117–1123.
Peters, G., Locci, R., Pulverer, G.: Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. Journal of Infectious Diseases 1982, 146: 479–482.
Hogt, A. H., Dankert, J., De Vries, J. A., Feijen. J.: Adhesion of coagulase-negative staphylococci to biomaterials. Journal of General Microbiology 1983, 129: 2959–2968.
Marrie, T. J., Noble, M. A., Costerton, J. W.: Examination of the morphology of bacteria adhering to peritoneal dialysis catheters by scanning and transmission electron microscopy. Journal of Clinical Microbiology 1983, 18: 1388–1398.
Marrie, T. J., Costerton, J. W.: Scanning and transmission electron microscopy of in situ bacterial colonization of intravenous and intraarterial catheters. Journal of Clinical Microbiology 1984, 19: 687–693.
Franson, T. R., Sheth, N. K., Rose, H. D., Sohnle, P. G.: Quantitative adherence in vitro of coagulase-negative staphylococci to intravascular catheters: inhibition with D-mannosamine. Journal of Infectious Diseases 1984, 149: 116.
Ludwicka, A., Jansen, B., Wadström, T., Pulverer, G.: Attachment of staphylococci to various synthetic polymers. Zentralblatt für Bakteriologie und Hygiene 1984, A 256: 479–489.
Christensen, G. D., Simpson, W. A., Younger, J. J., Baddour, L. M., Barrett, F. F., Melton, D. M., Beachey, E. H.: Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of Clinical Microbiology 1985, 22: 996–1006.
Hogt, A. H., Dankert, J., Hulstaert, C. E., Feijen, J.: Cell surface characteristics of coagulase-negative staphylococci and their adherence to fluorinated poly(ethylenepropylene). Infection and Immunity 1986, 51: 294–301.
Ashkenazi, S., Mirelman, D.; Adherence of bacteria to pediatric intravenous catheters and needles and its relation to phlebitis in animals. Pediatric Research 1984, 18: 1361–1366.
Kloos,W. W., Schleifer, K. H.: Simplified scheme for routine identification of human staphylococcus species. Journal of Clinical Microbiology 1975, 1: 82–88.
Rosenberg, M., Gutnick. D., Rosenberg, E.: Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiology Letters 1980, 9: 29–33.
Klotz, S. A., Drutz, D. J., Zajic, J. E.: Factors governing adherence of Candida species to plastic surfaces. Infection and Immunity 1985, 50: 97–101.
Christensen, G. D., Simpson, W. A., Bisno, A. L., Beachey, E. H.: Adherence of slime-producing strains ofStaphylococcus epidermidis to smooth surfaces. Infection and Immunity 1982, 37: 318–326.
Grinnell, F., Feld, M. K.: Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium. Journal of Biological Chemistry 1982, 257: 4888–4893.
Vercellotti, G. M., Lussenhop, D., Peterson, P. K., Furcht, L. T., McCarthy, J. B., Jacob, H. J., Moldow, C. F.: Bacterial adherence to fibronectin and endothelial cells: a possible mechanism for bacterial tissue tropism. Journal of Laboratory and Clinical Medicine 1984, 103: 34–43.
Vaudaux, P. E., Waldvogel, F. A., Morgenthaler, J. J., Nydegger, U. E.: Adsorption of fibronectin onto polymethylmethacrylate and promotion ofStaphylococcus aureus adherence. Infection and Immunity 1984, 45: 768–774.
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Pascual, A., Fleer, A., Westerdaal, N.A.C. et al. Modulation of adherence of coagulase-negative staphylococci to teflon catheters in vitro. Eur. J, Clin. Microbiol. 5, 518–522 (1986). https://doi.org/10.1007/BF02017694
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DOI: https://doi.org/10.1007/BF02017694