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The human leukemia cell line, THP-1: A multifacetted model for the study of monocyte-macrophage differentiation

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Summary

THP-1 is a human monocytic leukemia cell line. After treatment with phorbol esters, THP-1 cells differentiate into macrophage-like cells which mimic native monocyte-derived macrophages in several respects. Compared to other human myeloid cell lines, such as HL-60, U937, KG-1, or HEL cell lines, differentiated THP-1 cells behave more like native monocyte-derived macrophages. Because of these characteristics, the THP-1 cell line provides a valuable model for studying the mechanisms involved in macrophage differentiation, and for exploring the regulation of macrophage-specific genes as they relate to physiological functions displayed by these cells.

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Abbreviations

apo:

apolipoprotein

CFU-GM:

colony forming unit for the granulocyte-macrophage

CSF:

colony stimulating factor

FcR:

IgG Fc receptor

HMG-CoA reductase:

3-hydroxy 3-methylglutaryl Coenzyme A reductase

γ-IFN:

interferon

IL:

interleukin

LDL:

low density lipoprotein

mAb:

monoclonal antibody

PKC:

protein kinase C

PMA:

phorbol 12-myristate 13-acetate

TNF:

tumor necrosis factor-α

Literatur

  1. Adams, D. O., and Hamilton, T. A., The cell biology of macrophage activation. A., Rev. Immunol.2 (1984) 283–318.

    Google Scholar 

  2. Altieri, D. C., and Edgington, T. S., Sequential receptor cascade for coagulation proteins on monocytes. Constitutive biosynthesis and functional prothrombinase activity of membrane form of factor V/Va, J. biol. Chem.264 (1989) 2969–2972.

    Google Scholar 

  3. Anderson, C. L., and Looney, R. J., Human leucocyte IgG Fc receptors, Immunol. Today7 (1986) 264–266.

    Google Scholar 

  4. Angel, P., Allegretto, E. A., Okino, S. T., Hattori, K., Boyle, W. J., Hunter, T., and Karin, M., Oncogene jun encodes a sequence specific trans-activator similar to AP-1. Nature332 (1988) 166–171.

    Google Scholar 

  5. Arend, W. P., Ammons, J. T., and Kotzin, B. L., Lipopolysaccharide and interleukin1 inhibit interferon-gamma-induced Fc receptor expression on human monocytes. J. Immunol.139 (1987) 1873–1879.

    Google Scholar 

  6. Arenzana-Seisdedos, F., Mogensen, S. C., Vuillier, F., Fiers, W., and Virelizier, J. L., Autocrine secretion of tumor necrosis factor under the influence of interferon-gamma amplifies HLA-DR gene induction in human monocytes. Proc. natl Acad. Sci. USA85 (1988) 6087–6091.

    Google Scholar 

  7. Auwerx, J., Chait, A., and Deeb, S. S., Transcriptional regulation of the LDL-receptor and HMG-CoA reductase genes by protein kinase C and a putative negative regolatory protein. Proc. natl Acad. Sci. USA86 (1989) 1133–1137.

    Google Scholar 

  8. Auwerx, J., Chait, A., Wolfbauer, G., and Deeb, S., Involvement of second messengers in regulation of the low-density lipoprotein receptor gene. Molec. cell. Biol.9 (1989) 2298–2302.

    Google Scholar 

  9. Auwerx, J., Deeb, S., Brunzell, J. D., Peng, R., and Chait, A., Transcriptional activation of the lipoprotein lipase and apolipoprotein E genes accompanies differentiation in some human macrophage-like cell lines. Biochemistry27 (1988) 2651–2655.

    Google Scholar 

  10. Auwerx, J., Deeb, S., Brunzell, J. D., Wolfbauer, G., and Chait, A., Lipoprotein lipase gene expression in THP-1 cells. Biochemistry28 (1989) 4563–4567.

    Google Scholar 

  11. Auwerx, J., Staels, B., and Sassone-Corsi, P., Coupled and uncoupled induction of fos and jun transcription by different second messengers in cells of hematopoietic origin. Nucl. Acids Res.18 (1990) 221–228.

    Google Scholar 

  12. Auwerx, J., Staels, B., Van Vaeck, F., Verhoeven, G., and Ceuppens, J., IgG Fc receptor expression during macrophage differentiation of the monocytic leukemia cell line, THP-1. (1990) submitted.

  13. Basu, S. K., Brown, M. S., Ho, Y. K., Havel, R. J., and Goldstein, J. L., Mouse macrophages synthesize and secrete a protein resembling apolipoprotein E. Proc. natl Acad. Sci. USA78 (1981) 7545–7549.

    Google Scholar 

  14. Baumgartner, I., Scheiner, O., Holzinger, G., Boltz-Nitulescu, G., Klech, H., Lassmann, H., Rumpold, H., Forster, O., and Kraft, D., Expression of the VEP 13 antigen (CD16) on native human alveolar macrophages and cultured blood monocytes. Immunobiology177 (1988) 317–335.

    Google Scholar 

  15. Biddison, W. E., Rao, P. E., Talle, M. A., Goldstein, G., and Shaw, S., Possible involvement of the OKT4 molecule in T cell recognition of class II HLA antigens. J. exp. Med.156 (1982) 1065–1076.

    Google Scholar 

  16. Bishop, J. M., Viral oncogenes, Cell42 (1985) 23–38.

    Google Scholar 

  17. Bohmann, D., Bos, T. J., Admon, A., Nishimura, T., Vogt, P. K. and Tijan, R., Human protooncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1 Science238 (1987) 1386–1392.

    Google Scholar 

  18. Bravo, R., Neuberg, M., Burckhardt, J., Almendral, J., Wallich, R., and Muller, R., Involvement of common and cell type-specific pathways in c-fos gene control: stable induction by cAMP in macrophages. Cell48 (1987) 251–260.

    Google Scholar 

  19. Brelvi, Z., and Studzinski, G., Inhibition of DNA synthesis by an inducer of differentiation of leukemic cells, 1α, 25 dihydroxyvitamin D3, precedes down regulation of the c-myc gene. J. Cell Physiol.128 (1986) 171–179.

    Google Scholar 

  20. Brown, M. S., and Goldstein, J. L., A receptor-mediated pathway for cholesterol homeostasis. Science232 (1986), 34–47.

    Google Scholar 

  21. Brown, M. S., and Goldstein, J. L., Lipoprotein metabolism in the macrophage: implications for cholesterol deposition in atherosclerosis. A. Rev. Biochem.52 (1983) 223–261.

    Google Scholar 

  22. Brown, M. S., Goldstein, J. L., Krieger, M., Ho, Y. K., and Anderson, R. G. W., Reversible accumulation of cholesteryl esters in macrophages incubated with acetylated lipoproteins. J. Cell Biol.82 (1979) 597–613.

    Google Scholar 

  23. Byrne, P. V., Human myeloid cells possessing high affinity receptors for granulocyte-macrophage colony stimulating factor. Leuk. Res.13 (1989) 117–126.

    Google Scholar 

  24. Calabretta, B., Dissociation of c-fos induction from macrophage differentiation in human myeloid leukemia cell lines. Molec. cell. Biol.7 (1987) 769–774.

    Google Scholar 

  25. Ceuppens, J. L., Baroja, M. L., Van Vaeck, F., and Anderson, C. L., Defect in the membrane expression of high affinity 72-kD Fc gamma receptors on phagocytic cells in four healthy subjects. J. clin. Invest.82 (1988) 571–578.

    Google Scholar 

  26. Chait, A., Iverius, P. H., and Brunzell, J. D., Lipoprotein lipase secretion by human monocyte-derived macrophages. J. clin. Invest.69 (1982) 490–493.

    Google Scholar 

  27. Chiu, R., Boyle, W. J., Meek, J., Smeal, T., Hunter, T., and Karin, M., The c-fos protein interacts with c-jun/AP-1 to stimulate transcription of AP-1 responsive genes. Cell54 (1988) 541–552.

    Google Scholar 

  28. Clarkson, S. B., and Ory, P. A., CD16; developmentally regulated IgG Fc receptors on cultured human monocytes. J. exp. Med.167 (1988) 408–420.

    Google Scholar 

  29. Collins, S. J., The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression. Blood70 (1987) 1233–1244.

    Google Scholar 

  30. Dawson, P. A., Hofmann, S. L., van de Westhuyzen, D. R., Sudhoff, T. C., Brown, M. S., and Goldstein, J. L., Sterol-dependent repression of low density lipoprotein receptor promotor mediated by 16-base pair sequence adjacent to binding site for transcription factor Sp1. J. biol. Chem.263 (1988) 3372–3379.

    Google Scholar 

  31. Dinarello, C. A., Biology of interleukin 1. FASEB J.2 (1988) 108–115.

    Google Scholar 

  32. Faltynek, C. R., Finch, L. R., Miller, P., and Overton, W. R., Treatment with recombinant IFN-gamma decreases cell surface CD4 levels on peripheral blood monocytes and on myelomonocyte cell lines. J. Immunol.142 (1989) 500–508.

    Google Scholar 

  33. Fenton, M. J., Clark, B. D., Collins, K. L., Webb, A. C., Rich, A. C., and Auron, P. E., Transcriptional regulation of the human prointerleukin 1 beta gene. J. Immunol.138 (1987) 3972–3979.

    Google Scholar 

  34. Fenton, M. J., Vermeulen, M. W., Clark, B. D., Webb, A. C., and Auron, P. E., Human pro-IL-1 beta gene expression in monocytic cells is regulated by two distinct pathways. J. Immunol.140 (1988) 2267–2273.

    Google Scholar 

  35. Fleit, H. B., Wright, S. D., Durie, C. J., Valinsky, J. E., and Unkeless, J. C., Ontogeny of Fc receptors and complement receptor (CR3) during human myeloid differentiation. J. clin. Invest.73 (1984) 516–525.

    Google Scholar 

  36. Gaffney, E. V., Dell'Aquila, M. L., Lingenfelter, S. E., Huffnagle, G. B., and Wiest, D. L., Characterization of a colony-stimulating factor produced by the human monocytic leukemia cell line, THP-1. J. Leukoc. Biol.39 (1986) 409–421.

    Google Scholar 

  37. Gaffney, E. V., Lingenfelter, S. E., Koch, G. A., Lisi, P. J., Chu, C. W., and Tsai, S. C., Regulation by interferon gamma of function in the acute monocytic leukemia cell line, THP-1. J. Leukoc. Biol.43 (1988) 248–255.

    Google Scholar 

  38. Gee, C., Griffin, J., Sastre, L., Miller, L., Springer, T., Peivnica-Worms, H., and Roberts, T., Differentiation of myeloid cells is accompanied by increased levels of pp60c-arc protein and kinase activity. Proc. natl Acad. Sci. USA83 (1986) 5131–5135.

    Google Scholar 

  39. Giminez-Gallego, G., Rodkey, J., Benet, C., Rios-Candelore, M., DiSalvo, J., and Thomas, K., Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies. Science230 (1985) 1385–1388.

    Google Scholar 

  40. Gonda, T.J., and Metcalf, D., Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukemia. Nature310 (1984), 249–251.

    Google Scholar 

  41. Gondo, H., Kudo, J., White, J. W., Barr, C., Selvanayagam, P., and Saunders, G. I., Differential expression of the human thymosin-beta 4 gene in lymphocytes, macrophages, and granulocytes. J. Immunol.139 (1987) 3840–3848.

    Google Scholar 

  42. Gowda, S., Koler, R., and Bagby, G., Regulation of c-myc expression during growth and differentiation of normal leukemic human myeloid progenitor cells. J. clin. Invest.77 (1986) 271–276.

    Google Scholar 

  43. Hara, H., Tanishita, H., Yokayana, S., Tajima, S., and Yamamoto, A., Induction of acetylated low density lipoprotein receptor and suppression of low density lipoprotein receptor on the cells of a human monocytic leukemia cell line. Biochem. biophys. Res. Commun.146 (1987) 802–808.

    Google Scholar 

  44. Hsu, S. M., and Hsu, P. L., Aberrant expression of T cell and B cell markers in myelocyte/monocyte/histiocyte-derived lymphoma and leukemia cells. Is the infrequent expression of T/B cell markers sufficient to establish a lymphoid origin for Hodgkin's Reed-Sternberg cells? Am. J. Path.134 (1989) 203–212.

    Google Scholar 

  45. Ignotz, R. A., Heino, J., and Massague, J., Regulation of cell adhesion receptors by transforming growth factor-beta. Regulation of vitronectin receptor and LFA-1. J. biol. Chem.264 (1989) 389–392.

    Google Scholar 

  46. Johnston, R. B., Monocytes and macrophages. N. Engl. J. Med.318 (1988) 747–751.

    Google Scholar 

  47. Khoo, J. C., Mahoney, E. M., and Witztum, J. L., Secretion of lipoprotein lipase by macrophages in culture. J. biol. Chem.256 (1981) 7105–7108.

    Google Scholar 

  48. Kinet, J. P., Antibody-cell interactions: Fc receptors. Cell57 (1989) 351–354.

    Google Scholar 

  49. Kodama, T., Freeman, M., Rohrer, L., Zabrecky, J., Matsudaira, P., and Krieger, M., Type I macrophage scavenger receptor containsα-helical and collagen-like coiled coils. Nature343 (1990) 531–535.

    Google Scholar 

  50. Kodama, T., Reddy, P., Kishimoto, C., and Krieger, M., Purification and characterization of a bovine acetyl low density lipoprotein receptor. Proc. natl Acad. Sci. USA85 (1988) 9238–9242.

    Google Scholar 

  51. Koeffler, P., Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood62 (1983) 709–721.

    Google Scholar 

  52. Kostura, M. J., Tocci, M. J., Limjucco, G., Chin, J., Cameron, P., Hillman, A. G., Chartrain, N. A., and Schmidt, J. A., Identification of a monocyte specific pre-interleukin 1 beta convertase activity. Proc. natl. Acad. Sci. USA86 (1989) 5227–5231.

    Google Scholar 

  53. Kouzarides, T., and Ziff, E., The role of the leucine zipper in the fos-jun interaction. Nature336 (1988) 646–651.

    Google Scholar 

  54. Kovacs, E. J., Oppenheim, J. J., Carter, D. B., and Young, H. A., Enhanced interleukin-1 production by human monocytic cell lines following treatment with 5-azacytidine. J. Leukoc. Biol.41 (1987) 40–46.

    Google Scholar 

  55. Krakauer, T., Biochemical characterization of interleukin 1 from a human monocytic cell line. J. Leukoc. Biol.37 (1985) 511–518.

    Google Scholar 

  56. Kurata, H., Akiyama, H., Taniyama, T., and Marunouchi, T., Dose-dependent regulation of macrophage differentiation by mos mRNA in a human monocytic cell line. EMBO J.8 (1989) 457–463.

    Google Scholar 

  57. Lamph, W. W., Wamsley, P., Sassone-Corsi, P., and Verma, I., Induction of proto-oncogene jun/AP-1 by serum and TPA. Nature334 (1988) 629–631.

    Google Scholar 

  58. Landschulz, W. H., Johnson, P. F., and McKnight, S. L., The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite. Science243 (1989) 1681–1688.

    Google Scholar 

  59. Lee, J., Mehta, K., Blick, M. B., Gutterman, J. U., and Lopez-Berstein, G., Expression of c-fos, c-myb, and c-myc in human monocytes: correlation with monocytic differentiation. Blood69 (1987) 1542–1545.

    Google Scholar 

  60. Liesveld, J. L., Abboud, C. N., Looney, R. J., Ryan, D. H., and Brennan, J. K., Expression of IgG Fc receptors in myeloid leukemic cell lines. Effect of colony-stimulating factors and cytokines. J. Immunol.140 (1988) 1527–1533.

    Google Scholar 

  61. Lubbert, M., and Koeffler, H. P., Myeloid cell lines: tools for studying differentiation of normal and abnormal hematopoietic cells. Blood. Rev2 (1988) 121–133.

    Google Scholar 

  62. Maddon, P. J., Dalgleish, A. G., McDougal, J. S., Clapham, P. R., Weiss, R. A., and Axel, R., The T4 gene encodes the AIDS virus receptor and is expressed in the immune system of the brain. Cell47 (1986) 333–348.

    Google Scholar 

  63. Maeda, N., Hamasato, S., Miyazawa, H., Takata, M., Yamamoto, H., and Fujimoto, S., Augmentation of human cytotoxic T lymphocytes against autologous tumor by a factor released from human monocytic leukemia cell line. Jap. J. Cancer Res.80 (1989) 537–545.

    Google Scholar 

  64. Mahley, R. W., Apolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science240 (1988) 622–630.

    Google Scholar 

  65. Matsushima, K., Copeland, T. D., Onozaki, K., and Oppenheim, J. J., Purification and biochemical characteristics of two distinct human interleukins from the human myelomonocytic THP-1 cell line. Biochemistry25 (1986) 3424–3429.

    Google Scholar 

  66. Matsushima, K., Larsen, C. G., Dubois, G. C., and Oppenheim, J. J., Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J. exp. Med.169 (1989) 1485–1490.

    Google Scholar 

  67. Menju, M., Tajima, S., and Yamamoto, A., Expression of the apolipoprotein E gene in a human macrophage-like cell line, THP-1. J. Biochem.106 (1989) 505–510.

    Google Scholar 

  68. Metcalf, D., Hematopoietic growth factors. Lancet1 (1989) 825–827.

    Google Scholar 

  69. Metchnikoff, E., Immunity to Infectious Disease. Cambridge University Press. Cambridge/London/New York 1905.

    Google Scholar 

  70. Mitchell, R., Henning-Chubb, C., Huberman, E., and Verma, I., c-fos expression is neither sufficient nor obligatory for differentiation of monomyelocytes to macrophages. Cell45 (1986) 497–504.

    Google Scholar 

  71. Mitchell, R. L., Zokas, L., Schreiber, R. D., and Verma, I., Rapid induction of the expression of the proto-oncogene fos during human monocytic differentiation. Cell40 (1985) 209–217.

    Google Scholar 

  72. Mitchinson, M. J., and Ball, R. Y., Macrophages and atherogenesis. Lance2 (1987) 146–149.

    Google Scholar 

  73. Molina, J. M., Scadden, D. T., Byrn, R., Dinarello, C. A., and Groopman, J. E., Production of tumor necrosis factor alpha and interleukin 1 beta by monocytic cells infected with human immunodeficiency virus. J. clin. Invest.84 (1989) 733–737.

    Google Scholar 

  74. Muller, R., Curran, T., Muller, D., and Guilbert, L., Rapid induction of the expression of proto-oncogene fos during human monocytic differentiation. Nature314 (1985) 546–548.

    Google Scholar 

  75. Muller, R., Muller, D., and Guilbert, D., Differential expression of c-fos in hematopoietic cells: correlation with differentiation of monomyelocytic cells in vitro. EMBO J.3 (1984) 1887–1890.

    Google Scholar 

  76. Munker, R., Norman, A. W., and Koeffler, H. P., Vitamin D compounds. Effect on clonal proliferation and differentiation of human myeloid cells. J. clin. Invest.78 (1986) 424–430.

    Google Scholar 

  77. Murata, M., Onomichi, K., Eto, Y., Shibai, H., and Muramatsu, M., Expression of erythroid differentiation factor (EDF) in chinese hamster ovary cells. Biochem. biophys. Res. Commun.151 (1988) 230–235.

    Google Scholar 

  78. Nathan, C., Secretory products of macrophages. J. clin. Invest.79 (1987) 319–326.

    Google Scholar 

  79. Olivecrona, T., and Bengtsson-Olivecrona, G., Lipoprotein lipase from milk-the model enzyme in lipoprotein lipase research, in: Lipoprotein Lipase, pp. 15–58. Ed. J. Borensztajr. Evener press, Chicago 1987.

    Google Scholar 

  80. Opdenakker, G., Cabeza-Arvelaiz, Y., and Van Damme, J., Interaction of interferon with other cytokines. Experientia45 (1989) 513–520.

    Google Scholar 

  81. Osborne, T. F., Goldstein, J. L., and Brown, M. S., 5′ End of HMG CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcription. Cell42 (1985) 203–212.

    Google Scholar 

  82. Portillo, G., Turner, M., Chantry, D., and Feldmann, M., Effect of cytokines on HLA-DR and IL-1 production by a monocytic tumour THP-1. Immunology66 (1989) 170–175.

    Google Scholar 

  83. Ragsdale, C. G., and Arend, W. P., Loss of Fc receptor activity after culture of human monocytes on surface-bound immune complexes. J. exp. Med.151 (1980) 32–44.

    Google Scholar 

  84. Rauscher, F. J., Cohen, D. R., Curran, T., Bos, T. J., Vogt, P. K., Bohman, D., Tijan, R., and Franza, B. R., Fos-associated protein p39 is the product of the jun proto-oncogene. Science240 (1988) 1010–1016.

    Google Scholar 

  85. Reitsma, P., Rothberg, P., Astrin, S., Trial, J., Bar-Shavit, Z., Hall, S., Teitelbaum, S., and Kahn, A., Regulation of myc gene expression in HL-60 leukemia cells by a vitamin D metabolite. Nature306 (1983) 492–494.

    Google Scholar 

  86. Rohrer, L., Freeman, M., Kodama, T., Penman, M., and Krieger, M., Coiled-coil fibrous domains mediate ligand binding by macrophage scavenger receptor type II. Nature343 (1990) 570–572.

    Google Scholar 

  87. Ross, R., Atherosclerosis: a problem of the biology of the arterial wall cells and their interactions with blood components. Arteriosclerosis1 (1981) 293–311.

    Google Scholar 

  88. Ryseck, R. P., Hirai, S. I., Yaniv, M., and Bravo, R., Transcriptional activation of c-jun during G0/G1 transition in mouse fibroblasts. Nature334 (1988) 535–537.

    Google Scholar 

  89. Sariban, E., and Kufe, D., Expression of the platelet-derived growth factor 1 and 2 genes in human myeloid cell lines and monocytes. Cancer Res.48 (1988) 4498–4502.

    Google Scholar 

  90. Sariban, E., Mitchell, T., and Kufe, D., Expression of the c-fms protooncogene during human monocytic differentiation. Nature316 (1985) 64–66.

    Google Scholar 

  91. Sassone-Corsi, P., Lamph, W. W., Kamps, M., and Verma, I., Fos-associated cellular p39 is related to nuclear transcription factor AP-1. Cell54 (1988) 553–560.

    Google Scholar 

  92. Schmidt, J.A., and Abdulla, E., Down-regulation of IL-1 beta biosynthesis by inducers of the heat-shock response. J. Immunol.141 (1988) 2027–2034.

    Google Scholar 

  93. Schwartz, S. M., and Reidy, M., Common mechanisms of proliferation of smooth muscle in atherosclerosis and hypertension. Hum. Path.18 (1987) 240–247.

    Google Scholar 

  94. Sherman, M. L., Stone, R. M., Datta, R., Bernstein, S. H., and Kufe, D. W., Transcriptional and post-transcriptional regulation of c-jun expression during monocytic differentiation of human myeloid leukemic cells. J. biol. Chem.265 (1990) 3320–3323.

    Google Scholar 

  95. Sherr, C. J., Rettenmier, C. W., Sacca, R., Roussel, M. F., Look, A. T., and Stanley, E. R., The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1. Cell41 (1985) 665–676.

    Google Scholar 

  96. Sieff, C. A., Hematopoietic growth factors. J. clin. Invest.79 (1987) 1549–1557.

    Google Scholar 

  97. Smithgall, T. E., Yu, G., and Glazer, R. I., Identification of the differentiation-associated p93 tyrosine kinase of HL-60 leukemia cells as the product of the human c-fes locus and its expression in myelomonocytic cells. J. biol. Chem.263 (1988) 15050–15055.

    Google Scholar 

  98. Sudhof, T. C., Russell, D. W., Brown, M. S., and Goldstein, J. L., 42 bp element from LDL receptor gene confers end-product repression by sterols when inserted into viral TK promotor. Cell48 (1987) 1061–1069.

    Google Scholar 

  99. Tajima, S., Hayashi, R., Tsuchiya, S., Miyake, Y., and Yamamoto, A. Cells of human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase. Biochem. biophys. phys. Res. Commun.126 (1985) 526–531.

    Google Scholar 

  100. Tsuchiya, S., Yamabe, M., Yamaguchi, Y., Kobayashi, Y., Konno, T., and Tada, K., Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer26 (1980) 171–176.

    Google Scholar 

  101. Turner, M., Chantry, D., and Feldmann, M., Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. Biochem. biophys. Res. Commun.156 (1988) 830–839.

    Google Scholar 

  102. Unanue, E. R., and Allen, P. M., The basis for the immunoregulatory role of macrophages and other accessory cells. Science236 (1987) 551–557.

    Google Scholar 

  103. Unkeless, J. C., Function and heterogeneity of human Fc receptors for immunoglobulin G. J. clin. Invest.83 (1989) 355–361.

    Google Scholar 

  104. Van de Water, L., Aronson, D., and Braman, V., Alteration of fibronectin receptors in phorbolester treated human promonocytic leukemia cells. Cancer Res.48 (1988) 5730–5737.

    Google Scholar 

  105. van Furth, R., Cohn, Z. A., Hirsch, J. G., Humphrey, J. H., Spector, W. G., and Langevoort, H. L., The mononuclear phagocyte system: a new classification of macrophages, monocytes, and their precursor cells. Bull. WHO46 (1972) 845–852.

    Google Scholar 

  106. van Furth, R., Mononuclear phagocytes in immunity, infection, and pathology. Oxford Blackwell Scientific, Oxford 1975.

    Google Scholar 

  107. Verma, I. M., and Sassone-Corsi, P., Proto-Oncogene fos: complex but versatile regulation. Cell51 (1987) 513–514.

    Google Scholar 

  108. Via, D. P., Dresel, H. A., Cheng, S.-I., and Gotto, A. M., Murine macrophage tumors are a source of a 260-Dalton acetyl-low density lipoprotein receptor. J. biol. Chem.260 (1985) 7379–7386.

    Google Scholar 

  109. Via, D. P., Pons, L., Dennison, D. K., Fanslow, A. E., and Bernini F., Induction of acetyl-LDL receptor activity by phorbol ester in human monocyte cell line, THP-1. J. Lipid Res.30 (1989) 1515–1524.

    Google Scholar 

  110. Wang-Iverson, P., Gibson, J. C., and Brown, W. V., Plasma apolipoprotein E secretion by human monocyte derived macrophages. Biochim. biophys. Acta834 (1985) 256–262.

    Google Scholar 

  111. Westin, E., Wong-Staal, F., Gelmann, E., Dalla-Favera, R., Papas, T., Lautenberger, J., Allesandra, E., Reddy, E., Tronick, S., Aaronson, S., and Gallo, R., Expression of cellular homologues of retroviral onc genes in human hematopoietic cells. Proc. natl Acad. Sci. USA79 (1982) 2490–2495.

    Google Scholar 

  112. Yoshimasa, T., Sibley, D. R., Bouvier, M., Lefkowitz, R. J., and Caron, M. G., Cross-talk between cellular signalling pathways suggested by phorbol-ester induced adenylate cyclase phosphorylation. Nature327 (1987) 67–70.

    Google Scholar 

  113. Yu, G., Smitgall, T. E., and Glazer, R. I., K562 cells transfected with the human c-fes gene acquire the ability to undergo myeloid differentiation. J. biol. Chem.264 (1989) 10276–10281.

    Google Scholar 

  114. Zannis, V. I., Cole, F. S., Jackson, C. L., Kurnit, D. M., and Karathanasis, S. K., Distribution of apolipoprotein A-I, C-II, C-III, and E mRNA in human fetal tissues. Time-dependent induction of apolipoprotein E mRNA by cultures of human monocyte-macrophages. Biochemistry24 (1985) 4450–4455.

    Google Scholar 

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  1. Department of Developmental Biology, Laboratory of Experimental Medicine and Endocrinology, Campus Gasthuisberg, KU Leuven, B-3000, Leuven, Belgium

    J. Auwerx

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Auwerx, J. The human leukemia cell line, THP-1: A multifacetted model for the study of monocyte-macrophage differentiation. Experientia 47, 22–31 (1991). https://doi.org/10.1007/BF02041244

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  • DOI: https://doi.org/10.1007/BF02041244

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